Efficient vitrification of mouse embryos using the Kitasato Vitrification System as a novel vitrification device
Abstract Background Currently, the cryopreservation of embryos and oocytes is essential for assisted reproductive technology (ART) laboratories worldwide. This study aimed to evaluate the efficacy of the Kitasato Vitrification System (KVS) as a vitrification device for the cryopreservation of mouse...
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| Format: | Article |
| Language: | English |
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BMC
2017-04-01
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| Series: | Reproductive Biology and Endocrinology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12958-017-0249-2 |
| _version_ | 1811268077017366528 |
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| author | Kenji Momozawa Atsushi Matsuzawa Yukio Tokunaga Shiori Abe Yumi Koyanagi Miho Kurita Marina Nakano Takao Miyake |
| author_facet | Kenji Momozawa Atsushi Matsuzawa Yukio Tokunaga Shiori Abe Yumi Koyanagi Miho Kurita Marina Nakano Takao Miyake |
| author_sort | Kenji Momozawa |
| collection | DOAJ |
| description | Abstract Background Currently, the cryopreservation of embryos and oocytes is essential for assisted reproductive technology (ART) laboratories worldwide. This study aimed to evaluate the efficacy of the Kitasato Vitrification System (KVS) as a vitrification device for the cryopreservation of mouse embryos to determine whether this novel device can be adapted to the field of ART. Methods In Experiment 1, blastocysts were vitrified using the KVS. Vitrified blastocysts were warmed and subsequently cultured for 72 h. In Experiment 2, 2-cell-stage embryos were vitrified using the KVS, and vitrified embryos were warmed and subsequently cultured for 96 h. In Experiment 3, we evaluated the in vivo developmental potential of vitrified 2-cell-stage embryos using the KVS, and in Experiment 4, we evaluated the cooling and warming rates for these devices using a numerical simulation. Results In Experiment 1, there were no significant differences between the survival rates of the KVS and a control device. However, re-expanded (100%) and hatching (91.8%) rates were significantly higher for blastocysts vitrified using the KVS. In Experiment 2, there were no significant differences between the survival rates, or rates of development to the blastocyst stage, of vitrified and fresh embryos. In Experiment 3, after embryo transfer, 41% of the embryos developed into live offspring. In Experiment 4, the cooling and warming rates of the KVS were 683,000 and 612,000 °C/min, respectively, exceeding those of the control device. Conclusions Our study clearly demonstrates that the KVS is a novel vitrification device for the cryopreservation of mouse embryos at the blastocyst and 2-cell stage. |
| first_indexed | 2024-04-12T21:15:22Z |
| format | Article |
| id | doaj.art-b3ae4bb34eba4723a45670e0ffe065d8 |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-04-12T21:15:22Z |
| publishDate | 2017-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-b3ae4bb34eba4723a45670e0ffe065d82022-12-22T03:16:28ZengBMCReproductive Biology and Endocrinology1477-78272017-04-011511910.1186/s12958-017-0249-2Efficient vitrification of mouse embryos using the Kitasato Vitrification System as a novel vitrification deviceKenji Momozawa0Atsushi Matsuzawa1Yukio Tokunaga2Shiori Abe3Yumi Koyanagi4Miho Kurita5Marina Nakano6Takao Miyake7School of Veterinary Medicine, Kitasato UniversityKyoto R&D Laboratory, Mitsubishi Paper Mills LimitedKyoto R&D Laboratory, Mitsubishi Paper Mills LimitedMiyake Women’s ClinicMiyake Women’s ClinicMiyake Women’s ClinicMiyake Women’s ClinicMiyake Women’s ClinicAbstract Background Currently, the cryopreservation of embryos and oocytes is essential for assisted reproductive technology (ART) laboratories worldwide. This study aimed to evaluate the efficacy of the Kitasato Vitrification System (KVS) as a vitrification device for the cryopreservation of mouse embryos to determine whether this novel device can be adapted to the field of ART. Methods In Experiment 1, blastocysts were vitrified using the KVS. Vitrified blastocysts were warmed and subsequently cultured for 72 h. In Experiment 2, 2-cell-stage embryos were vitrified using the KVS, and vitrified embryos were warmed and subsequently cultured for 96 h. In Experiment 3, we evaluated the in vivo developmental potential of vitrified 2-cell-stage embryos using the KVS, and in Experiment 4, we evaluated the cooling and warming rates for these devices using a numerical simulation. Results In Experiment 1, there were no significant differences between the survival rates of the KVS and a control device. However, re-expanded (100%) and hatching (91.8%) rates were significantly higher for blastocysts vitrified using the KVS. In Experiment 2, there were no significant differences between the survival rates, or rates of development to the blastocyst stage, of vitrified and fresh embryos. In Experiment 3, after embryo transfer, 41% of the embryos developed into live offspring. In Experiment 4, the cooling and warming rates of the KVS were 683,000 and 612,000 °C/min, respectively, exceeding those of the control device. Conclusions Our study clearly demonstrates that the KVS is a novel vitrification device for the cryopreservation of mouse embryos at the blastocyst and 2-cell stage.http://link.springer.com/article/10.1186/s12958-017-0249-2VitrificationKVSEmbryosUltra-rapid coolingCryopreservation |
| spellingShingle | Kenji Momozawa Atsushi Matsuzawa Yukio Tokunaga Shiori Abe Yumi Koyanagi Miho Kurita Marina Nakano Takao Miyake Efficient vitrification of mouse embryos using the Kitasato Vitrification System as a novel vitrification device Reproductive Biology and Endocrinology Vitrification KVS Embryos Ultra-rapid cooling Cryopreservation |
| title | Efficient vitrification of mouse embryos using the Kitasato Vitrification System as a novel vitrification device |
| title_full | Efficient vitrification of mouse embryos using the Kitasato Vitrification System as a novel vitrification device |
| title_fullStr | Efficient vitrification of mouse embryos using the Kitasato Vitrification System as a novel vitrification device |
| title_full_unstemmed | Efficient vitrification of mouse embryos using the Kitasato Vitrification System as a novel vitrification device |
| title_short | Efficient vitrification of mouse embryos using the Kitasato Vitrification System as a novel vitrification device |
| title_sort | efficient vitrification of mouse embryos using the kitasato vitrification system as a novel vitrification device |
| topic | Vitrification KVS Embryos Ultra-rapid cooling Cryopreservation |
| url | http://link.springer.com/article/10.1186/s12958-017-0249-2 |
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