Genetic Clarification of Auricularia heimuer Strains Bred and Cultivated in Korea Using the ITS and IGS1 rDNA Region Sequences
AbstractAuricularia is one of the broadly cultivated edible mushrooms in Korea. Most of the Korean Auricularia strains used for cultivation and breeding are known as A. auricula-judae. Recently, this species has been reported to belong to a species complex. Therefore, this study was carried out to g...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-03-01
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| Series: | Mycobiology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/12298093.2023.2182024 |
| _version_ | 1827957970072240128 |
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| author | Nitesh Pant HyeongJin Noh Won-Ho Lee Seong Hwan Kim |
| author_facet | Nitesh Pant HyeongJin Noh Won-Ho Lee Seong Hwan Kim |
| author_sort | Nitesh Pant |
| collection | DOAJ |
| description | AbstractAuricularia is one of the broadly cultivated edible mushrooms in Korea. Most of the Korean Auricularia strains used for cultivation and breeding are known as A. auricula-judae. Recently, this species has been reported to belong to a species complex. Therefore, this study was carried out to genetically clarify the bred and cultivated Korean A. auricula-judae strains. The internal transcribed spacer (ITS) and IGS1 rDNA region sequences were determined from 10 A. auricula-judae strains by PCR and sequencing. Variation in the nucleotide sequence and sequence length of the two rDNA regions were found among the seven A. auricula-judae strains. A maximum-likelihood (ML) phylogenetic tree based on the ITS sequences clearly placed all the 10 Korean A. auricula-judae strains in the A. heimuer clade of the A. auricula-judae complex. A. heimuer is diverged from A. auricula-judae. An ML phylogenetic tree based on the IGS1 sequences revealed the close relationship between Korean A. heimuer strains to Chinese A. heimuer strains. But each strain could be distinguishable by the IGS1 sequence. Furthermore, progeny strains in the seven Korean strains could be differentiated from their parental strains by the IGS1 sequence based phylogenetic tree. Our results are expected to be used to complement the distinction of domestic Auricularia cultivars. |
| first_indexed | 2024-04-09T15:29:22Z |
| format | Article |
| id | doaj.art-b3aec90ff2214ad19c1845f1d2f35d94 |
| institution | Directory Open Access Journal |
| issn | 1229-8093 2092-9323 |
| language | English |
| last_indexed | 2024-04-09T15:29:22Z |
| publishDate | 2023-03-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mycobiology |
| spelling | doaj.art-b3aec90ff2214ad19c1845f1d2f35d942023-04-28T10:21:59ZengTaylor & Francis GroupMycobiology1229-80932092-93232023-03-0151210911310.1080/12298093.2023.2182024Genetic Clarification of Auricularia heimuer Strains Bred and Cultivated in Korea Using the ITS and IGS1 rDNA Region SequencesNitesh Pant0HyeongJin Noh1Won-Ho Lee2Seong Hwan Kim3Department of Microbiology, Division of Biological Sciences, Dankook University, Cheonan, Republic of KoreaDepartment of Microbiology, Division of Biological Sciences, Dankook University, Cheonan, Republic of KoreaForest Mushroom Research Center, Yeoju, Republic of KoreaDepartment of Microbiology, Division of Biological Sciences, Dankook University, Cheonan, Republic of KoreaAbstractAuricularia is one of the broadly cultivated edible mushrooms in Korea. Most of the Korean Auricularia strains used for cultivation and breeding are known as A. auricula-judae. Recently, this species has been reported to belong to a species complex. Therefore, this study was carried out to genetically clarify the bred and cultivated Korean A. auricula-judae strains. The internal transcribed spacer (ITS) and IGS1 rDNA region sequences were determined from 10 A. auricula-judae strains by PCR and sequencing. Variation in the nucleotide sequence and sequence length of the two rDNA regions were found among the seven A. auricula-judae strains. A maximum-likelihood (ML) phylogenetic tree based on the ITS sequences clearly placed all the 10 Korean A. auricula-judae strains in the A. heimuer clade of the A. auricula-judae complex. A. heimuer is diverged from A. auricula-judae. An ML phylogenetic tree based on the IGS1 sequences revealed the close relationship between Korean A. heimuer strains to Chinese A. heimuer strains. But each strain could be distinguishable by the IGS1 sequence. Furthermore, progeny strains in the seven Korean strains could be differentiated from their parental strains by the IGS1 sequence based phylogenetic tree. Our results are expected to be used to complement the distinction of domestic Auricularia cultivars.https://www.tandfonline.com/doi/10.1080/12298093.2023.2182024Auricularia heimuerA. auricula-judae complexITSIGS1 |
| spellingShingle | Nitesh Pant HyeongJin Noh Won-Ho Lee Seong Hwan Kim Genetic Clarification of Auricularia heimuer Strains Bred and Cultivated in Korea Using the ITS and IGS1 rDNA Region Sequences Mycobiology Auricularia heimuer A. auricula-judae complex ITS IGS1 |
| title | Genetic Clarification of Auricularia heimuer Strains Bred and Cultivated in Korea Using the ITS and IGS1 rDNA Region Sequences |
| title_full | Genetic Clarification of Auricularia heimuer Strains Bred and Cultivated in Korea Using the ITS and IGS1 rDNA Region Sequences |
| title_fullStr | Genetic Clarification of Auricularia heimuer Strains Bred and Cultivated in Korea Using the ITS and IGS1 rDNA Region Sequences |
| title_full_unstemmed | Genetic Clarification of Auricularia heimuer Strains Bred and Cultivated in Korea Using the ITS and IGS1 rDNA Region Sequences |
| title_short | Genetic Clarification of Auricularia heimuer Strains Bred and Cultivated in Korea Using the ITS and IGS1 rDNA Region Sequences |
| title_sort | genetic clarification of auricularia heimuer strains bred and cultivated in korea using the its and igs1 rdna region sequences |
| topic | Auricularia heimuer A. auricula-judae complex ITS IGS1 |
| url | https://www.tandfonline.com/doi/10.1080/12298093.2023.2182024 |
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