Oligosaccharide feed supplementation reduces plasma insulin in geldings with Equine Metabolic Syndrome
BackgroundThe prevalence of Equine Metabolic Syndrome and insulin dysregulation is increasing in the horse population. Insulin dysregulation is a risk factor for laminitis, which has significant welfare consequences for the horse. Current treatment strategies for Equine Metabolic Syndrome have varia...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Microbiomes |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/frmbi.2023.1194705/full |
| _version_ | 1797756459061084160 |
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| author | Alexandra von Münchow Alexandra von Münchow Sarah Torp Yttergren Rasmus Riemer Jakobsen Nanna Luthersson Axel Kornerup Hansen Frederikke Lindenberg |
| author_facet | Alexandra von Münchow Alexandra von Münchow Sarah Torp Yttergren Rasmus Riemer Jakobsen Nanna Luthersson Axel Kornerup Hansen Frederikke Lindenberg |
| author_sort | Alexandra von Münchow |
| collection | DOAJ |
| description | BackgroundThe prevalence of Equine Metabolic Syndrome and insulin dysregulation is increasing in the horse population. Insulin dysregulation is a risk factor for laminitis, which has significant welfare consequences for the horse. Current treatment strategies for Equine Metabolic Syndrome have variable effects on insulin sensitivity. Findings suggest that gut microbiota plays an important role in gastrointestinal barrier protection and metabolic diseases. Dietary supplementation with oligosaccharides has been shown to increase the production of short-chain fatty acids and induce changes in the gut microbiota, with an increase in insulin sensitivity.ObjectivesWe hypothesized that dietary oligosaccharide supplementation would ameliorate insulin dysregulation in horses with Equine Metabolic Syndrome.Study designFifteen horses were included in a cross-over study including two feed supplementations periods with oligosaccharide supplementation or calcium carbonate. Before and after each period plasma insulin, endotoxin, serum amyloid A, and blood glucose concentrations were measured during an oral sugar test and fecal samples were collected.ResultsOligosaccharide supplementation significantly reduced insulin in geldings (p = 0.02). Overall, none of the dominating bacterias changed significantly in relative Δ-abundance. In the gut microbiota of mares, the Akkermansia genus and Clostridiaceae family were significantly more abundant. Within-sample bacterial diversity of horses with insulin concentrations > 60 mIU/L was significantly greater when compared to horses < 60 mIU/L. Horses with insulin concentrations > 60 mIU/L and horses with previous laminitis had a significantly greater beta diversity.Main limitationsCut-off values for oligosaccharide feed supplementation/placebo dosing instead of dosing by g/kg body weight; owner compliance; single blood sample in oral sugar test; inter-horse variationConclusionsThis study demonstrated that oligosaccharide supplementation could improve insulin dysregulation in geldings with Equine Metabolic Syndrome suggesting that sex susceptibility might be a factor to consider. Additionally, a tendency of reduction of obesity-associated bacteria was observed. Furthermore, the diversity of the gut microbiota was significantly dependent on laminitis status and insulin concentrations. However, more studies on the effects of oligosaccharide supplementation on insulin dysregulation are needed to improve the dietary management of horses with Equine Metabolic Syndrome. |
| first_indexed | 2024-03-12T18:01:44Z |
| format | Article |
| id | doaj.art-b3b030a4a44e49f5b0479482cb7e5aa2 |
| institution | Directory Open Access Journal |
| issn | 2813-4338 |
| language | English |
| last_indexed | 2024-03-12T18:01:44Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiomes |
| spelling | doaj.art-b3b030a4a44e49f5b0479482cb7e5aa22023-08-02T10:06:23ZengFrontiers Media S.A.Frontiers in Microbiomes2813-43382023-08-01210.3389/frmbi.2023.11947051194705Oligosaccharide feed supplementation reduces plasma insulin in geldings with Equine Metabolic SyndromeAlexandra von Münchow0Alexandra von Münchow1Sarah Torp Yttergren2Rasmus Riemer Jakobsen3Nanna Luthersson4Axel Kornerup Hansen5Frederikke Lindenberg6Section of Experimental Animal Models, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, DenmarkHestedoktoren, Højgaard Sjælland ApS, Hvalsoe, DenmarkSection of Experimental Animal Models, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, DenmarkSection of Microbiology and Fermentation, Department of Food Sciences, Faculty of Science, University of Copenhagen, Copenhagen, DenmarkSection of Experimental Animal Models, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, DenmarkHestedoktoren, Højgaard Sjælland ApS, Hvalsoe, DenmarkBrogaarden ApS, Lynge, DenmarkBackgroundThe prevalence of Equine Metabolic Syndrome and insulin dysregulation is increasing in the horse population. Insulin dysregulation is a risk factor for laminitis, which has significant welfare consequences for the horse. Current treatment strategies for Equine Metabolic Syndrome have variable effects on insulin sensitivity. Findings suggest that gut microbiota plays an important role in gastrointestinal barrier protection and metabolic diseases. Dietary supplementation with oligosaccharides has been shown to increase the production of short-chain fatty acids and induce changes in the gut microbiota, with an increase in insulin sensitivity.ObjectivesWe hypothesized that dietary oligosaccharide supplementation would ameliorate insulin dysregulation in horses with Equine Metabolic Syndrome.Study designFifteen horses were included in a cross-over study including two feed supplementations periods with oligosaccharide supplementation or calcium carbonate. Before and after each period plasma insulin, endotoxin, serum amyloid A, and blood glucose concentrations were measured during an oral sugar test and fecal samples were collected.ResultsOligosaccharide supplementation significantly reduced insulin in geldings (p = 0.02). Overall, none of the dominating bacterias changed significantly in relative Δ-abundance. In the gut microbiota of mares, the Akkermansia genus and Clostridiaceae family were significantly more abundant. Within-sample bacterial diversity of horses with insulin concentrations > 60 mIU/L was significantly greater when compared to horses < 60 mIU/L. Horses with insulin concentrations > 60 mIU/L and horses with previous laminitis had a significantly greater beta diversity.Main limitationsCut-off values for oligosaccharide feed supplementation/placebo dosing instead of dosing by g/kg body weight; owner compliance; single blood sample in oral sugar test; inter-horse variationConclusionsThis study demonstrated that oligosaccharide supplementation could improve insulin dysregulation in geldings with Equine Metabolic Syndrome suggesting that sex susceptibility might be a factor to consider. Additionally, a tendency of reduction of obesity-associated bacteria was observed. Furthermore, the diversity of the gut microbiota was significantly dependent on laminitis status and insulin concentrations. However, more studies on the effects of oligosaccharide supplementation on insulin dysregulation are needed to improve the dietary management of horses with Equine Metabolic Syndrome.https://www.frontiersin.org/articles/10.3389/frmbi.2023.1194705/fullEquine Metabolic Syndromeprebioticsoligosaccharidefatty acidsvolatilegut microbiota |
| spellingShingle | Alexandra von Münchow Alexandra von Münchow Sarah Torp Yttergren Rasmus Riemer Jakobsen Nanna Luthersson Axel Kornerup Hansen Frederikke Lindenberg Oligosaccharide feed supplementation reduces plasma insulin in geldings with Equine Metabolic Syndrome Frontiers in Microbiomes Equine Metabolic Syndrome prebiotics oligosaccharide fatty acids volatile gut microbiota |
| title | Oligosaccharide feed supplementation reduces plasma insulin in geldings with Equine Metabolic Syndrome |
| title_full | Oligosaccharide feed supplementation reduces plasma insulin in geldings with Equine Metabolic Syndrome |
| title_fullStr | Oligosaccharide feed supplementation reduces plasma insulin in geldings with Equine Metabolic Syndrome |
| title_full_unstemmed | Oligosaccharide feed supplementation reduces plasma insulin in geldings with Equine Metabolic Syndrome |
| title_short | Oligosaccharide feed supplementation reduces plasma insulin in geldings with Equine Metabolic Syndrome |
| title_sort | oligosaccharide feed supplementation reduces plasma insulin in geldings with equine metabolic syndrome |
| topic | Equine Metabolic Syndrome prebiotics oligosaccharide fatty acids volatile gut microbiota |
| url | https://www.frontiersin.org/articles/10.3389/frmbi.2023.1194705/full |
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