Protocol for Immuno-Enrichment of FLAG-Tagged Protein Complexes
Summary: This protocol describes immunoprecipitation of proteins associated with FLAG-tagged recombinant proteins followed by mass spectrometry-based proteomics to identify the associated interactome components. FLAG epitope was chosen, because existing high-affinity monoclonal antibodies allow for...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2020-09-01
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Series: | STAR Protocols |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166720300708 |