Optimizing the Boosting Schedule of Subunit Vaccines Consisting of BCG and “Non-BCG” Antigens to Induce Long-Term Immune Memory
Boosting Bacillus Calmette-Guérin (BCG) with subunit vaccine is expected to induce long-term protection against tuberculosis (TB). However, it is urgently needed to optimize the boosting schedule of subunit vaccines, which consists of antigens from or not from BCG, to induce long-term immune memory....
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-04-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.862726/full |
| _version_ | 1819060337139253248 |
|---|---|
| author | Wei Lv Pu He Yanlin Ma Daquan Tan Fei Li Tao Xie Jiangyuan Han Juan Wang Youjun Mi Youjun Mi Hongxia Niu Bingdong Zhu Bingdong Zhu |
| author_facet | Wei Lv Pu He Yanlin Ma Daquan Tan Fei Li Tao Xie Jiangyuan Han Juan Wang Youjun Mi Youjun Mi Hongxia Niu Bingdong Zhu Bingdong Zhu |
| author_sort | Wei Lv |
| collection | DOAJ |
| description | Boosting Bacillus Calmette-Guérin (BCG) with subunit vaccine is expected to induce long-term protection against tuberculosis (TB). However, it is urgently needed to optimize the boosting schedule of subunit vaccines, which consists of antigens from or not from BCG, to induce long-term immune memory. To address it two subunit vaccines, Mtb10.4-HspX (MH) consisting of BCG antigens and ESAT6-CFP10 (EC) consisting of antigens from the region of difference (RD) of Mycobacterium tuberculosis (M. tuberculosis), were applied to immunize BCG-primed C57BL/6 mice twice or thrice with different intervals, respectively. The long-term antigen-specific immune responses and protective efficacy against M. tuberculosis H37Ra were determined. The results showed that following BCG priming, MH boosting twice at 12-24 weeks or EC immunizations thrice at 12-16-24 weeks enhanced the number and function of long-lived memory T cells with improved protection against H37Ra, while MH boosting thrice at 12-16-24 weeks or twice at 8-14 weeks and EC immunizations twice at 12-24 weeks or thrice at 8-10-14 weeks didn’t induce long-term immunity. It suggests that following BCG priming, both BCG antigens MH boosting twice and “non-BCG” antigens EC immunizations thrice at suitable intervals induce long-lived memory T cell-mediated immunity. |
| first_indexed | 2024-12-21T14:25:23Z |
| format | Article |
| id | doaj.art-b3b71931348443169aa562438c4c34a0 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-21T14:25:23Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-b3b71931348443169aa562438c4c34a02022-12-21T19:00:40ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-04-011310.3389/fimmu.2022.862726862726Optimizing the Boosting Schedule of Subunit Vaccines Consisting of BCG and “Non-BCG” Antigens to Induce Long-Term Immune MemoryWei Lv0Pu He1Yanlin Ma2Daquan Tan3Fei Li4Tao Xie5Jiangyuan Han6Juan Wang7Youjun Mi8Youjun Mi9Hongxia Niu10Bingdong Zhu11Bingdong Zhu12Gansu Provincial Key Laboratory of Evidence Based Medicine and Clinical Translation and Lanzhou Center for Tuberculosis Research, Institute of Pathogen Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, ChinaGansu Provincial Key Laboratory of Evidence Based Medicine and Clinical Translation and Lanzhou Center for Tuberculosis Research, Institute of Pathogen Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, ChinaGansu Provincial Key Laboratory of Evidence Based Medicine and Clinical Translation and Lanzhou Center for Tuberculosis Research, Institute of Pathogen Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, ChinaGansu Provincial Key Laboratory of Evidence Based Medicine and Clinical Translation and Lanzhou Center for Tuberculosis Research, Institute of Pathogen Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, ChinaGansu Provincial Key Laboratory of Evidence Based Medicine and Clinical Translation and Lanzhou Center for Tuberculosis Research, Institute of Pathogen Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, ChinaGansu Provincial Key Laboratory of Evidence Based Medicine and Clinical Translation and Lanzhou Center for Tuberculosis Research, Institute of Pathogen Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, ChinaGansu Provincial Key Laboratory of Evidence Based Medicine and Clinical Translation and Lanzhou Center for Tuberculosis Research, Institute of Pathogen Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, ChinaGansu Provincial Key Laboratory of Evidence Based Medicine and Clinical Translation and Lanzhou Center for Tuberculosis Research, Institute of Pathogen Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, ChinaGansu Provincial Key Laboratory of Evidence Based Medicine and Clinical Translation and Lanzhou Center for Tuberculosis Research, Institute of Pathogen Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, ChinaInstitute of Pathophysiology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, ChinaGansu Provincial Key Laboratory of Evidence Based Medicine and Clinical Translation and Lanzhou Center for Tuberculosis Research, Institute of Pathogen Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, ChinaGansu Provincial Key Laboratory of Evidence Based Medicine and Clinical Translation and Lanzhou Center for Tuberculosis Research, Institute of Pathogen Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, ChinaState Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University, Lanzhou, ChinaBoosting Bacillus Calmette-Guérin (BCG) with subunit vaccine is expected to induce long-term protection against tuberculosis (TB). However, it is urgently needed to optimize the boosting schedule of subunit vaccines, which consists of antigens from or not from BCG, to induce long-term immune memory. To address it two subunit vaccines, Mtb10.4-HspX (MH) consisting of BCG antigens and ESAT6-CFP10 (EC) consisting of antigens from the region of difference (RD) of Mycobacterium tuberculosis (M. tuberculosis), were applied to immunize BCG-primed C57BL/6 mice twice or thrice with different intervals, respectively. The long-term antigen-specific immune responses and protective efficacy against M. tuberculosis H37Ra were determined. The results showed that following BCG priming, MH boosting twice at 12-24 weeks or EC immunizations thrice at 12-16-24 weeks enhanced the number and function of long-lived memory T cells with improved protection against H37Ra, while MH boosting thrice at 12-16-24 weeks or twice at 8-14 weeks and EC immunizations twice at 12-24 weeks or thrice at 8-10-14 weeks didn’t induce long-term immunity. It suggests that following BCG priming, both BCG antigens MH boosting twice and “non-BCG” antigens EC immunizations thrice at suitable intervals induce long-lived memory T cell-mediated immunity.https://www.frontiersin.org/articles/10.3389/fimmu.2022.862726/fulltuberculosisBCGsubunit vaccineboost scheduleimmunization program |
| spellingShingle | Wei Lv Pu He Yanlin Ma Daquan Tan Fei Li Tao Xie Jiangyuan Han Juan Wang Youjun Mi Youjun Mi Hongxia Niu Bingdong Zhu Bingdong Zhu Optimizing the Boosting Schedule of Subunit Vaccines Consisting of BCG and “Non-BCG” Antigens to Induce Long-Term Immune Memory Frontiers in Immunology tuberculosis BCG subunit vaccine boost schedule immunization program |
| title | Optimizing the Boosting Schedule of Subunit Vaccines Consisting of BCG and “Non-BCG” Antigens to Induce Long-Term Immune Memory |
| title_full | Optimizing the Boosting Schedule of Subunit Vaccines Consisting of BCG and “Non-BCG” Antigens to Induce Long-Term Immune Memory |
| title_fullStr | Optimizing the Boosting Schedule of Subunit Vaccines Consisting of BCG and “Non-BCG” Antigens to Induce Long-Term Immune Memory |
| title_full_unstemmed | Optimizing the Boosting Schedule of Subunit Vaccines Consisting of BCG and “Non-BCG” Antigens to Induce Long-Term Immune Memory |
| title_short | Optimizing the Boosting Schedule of Subunit Vaccines Consisting of BCG and “Non-BCG” Antigens to Induce Long-Term Immune Memory |
| title_sort | optimizing the boosting schedule of subunit vaccines consisting of bcg and non bcg antigens to induce long term immune memory |
| topic | tuberculosis BCG subunit vaccine boost schedule immunization program |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.862726/full |
| work_keys_str_mv | AT weilv optimizingtheboostingscheduleofsubunitvaccinesconsistingofbcgandnonbcgantigenstoinducelongtermimmunememory AT puhe optimizingtheboostingscheduleofsubunitvaccinesconsistingofbcgandnonbcgantigenstoinducelongtermimmunememory AT yanlinma optimizingtheboostingscheduleofsubunitvaccinesconsistingofbcgandnonbcgantigenstoinducelongtermimmunememory AT daquantan optimizingtheboostingscheduleofsubunitvaccinesconsistingofbcgandnonbcgantigenstoinducelongtermimmunememory AT feili optimizingtheboostingscheduleofsubunitvaccinesconsistingofbcgandnonbcgantigenstoinducelongtermimmunememory AT taoxie optimizingtheboostingscheduleofsubunitvaccinesconsistingofbcgandnonbcgantigenstoinducelongtermimmunememory AT jiangyuanhan optimizingtheboostingscheduleofsubunitvaccinesconsistingofbcgandnonbcgantigenstoinducelongtermimmunememory AT juanwang optimizingtheboostingscheduleofsubunitvaccinesconsistingofbcgandnonbcgantigenstoinducelongtermimmunememory AT youjunmi optimizingtheboostingscheduleofsubunitvaccinesconsistingofbcgandnonbcgantigenstoinducelongtermimmunememory AT youjunmi optimizingtheboostingscheduleofsubunitvaccinesconsistingofbcgandnonbcgantigenstoinducelongtermimmunememory AT hongxianiu optimizingtheboostingscheduleofsubunitvaccinesconsistingofbcgandnonbcgantigenstoinducelongtermimmunememory AT bingdongzhu optimizingtheboostingscheduleofsubunitvaccinesconsistingofbcgandnonbcgantigenstoinducelongtermimmunememory AT bingdongzhu optimizingtheboostingscheduleofsubunitvaccinesconsistingofbcgandnonbcgantigenstoinducelongtermimmunememory |