Multistage Extraction of Star Anise and Black Pepper Derivatives for Antibacterial, Antioxidant, and Anticancer Activity
Recently, natural resources have attracted considerable interest for their applications in food security and human health problems. Traditional natural spices, such as star anise and black pepper, played important roles in the pharmaceutical and food industries due to their strong pharmacological ac...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-05-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2021.660138/full |
| _version_ | 1818921574015696896 |
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| author | Helin Li Helin Li Xiaoyu Wu Xin Li Xiaobing Cao Yanjun Li Huaru Cao Yongzhi Men |
| author_facet | Helin Li Helin Li Xiaoyu Wu Xin Li Xiaobing Cao Yanjun Li Huaru Cao Yongzhi Men |
| author_sort | Helin Li |
| collection | DOAJ |
| description | Recently, natural resources have attracted considerable interest for their applications in food security and human health problems. Traditional natural spices, such as star anise and black pepper, played important roles in the pharmaceutical and food industries due to their strong pharmacological activity, antioxidant potential and rare complications. In order to achieve biomasses from the natural product with multiple bioactivities, we developed the multistage extraction method to extract and separate various bioactive compounds from these natural plants. Our work demonstrated that various bioactive-rich extractives were achieved using steam distilled- or oxidative-extraction methods with high extraction yields and purity. Furthermore, the extractives in each step can be used not only as bioactive compounds, but also as a resource to further prepare different derivatives during the next extractive step, providing biomass-saving to a great extent. The extractives obtained with high yields and purities (>82%) were identified by 1H NMR, 13C NMR, FTIR, UV-vis, fluorescence spectroscopy, and high-performance liquid chromatography (HPLC). Moreover, these biomasses display potent antibacterial activities against some types of microorganisms such as S.aureus, S.pyogenes, E.coli, and S.typhi with a lowest MIC of 400 μg/ml for the development of antibacterial agents, significant antioxidant activity as the natural antioxidant for enhancing food shelf-life, and excellent anticancer activity that induces significant cancer cell apoptosis. This work showed the different multistage extracts from natural products, which enable them to be applied in the fields of the pharmaceutical industry and the food industry. |
| first_indexed | 2024-12-20T01:39:48Z |
| format | Article |
| id | doaj.art-b3bc78384fb241ea8d5d4729b30373c7 |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-12-20T01:39:48Z |
| publishDate | 2021-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-b3bc78384fb241ea8d5d4729b30373c72022-12-21T19:57:55ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462021-05-01910.3389/fchem.2021.660138660138Multistage Extraction of Star Anise and Black Pepper Derivatives for Antibacterial, Antioxidant, and Anticancer ActivityHelin Li0Helin Li1Xiaoyu Wu2Xin Li3Xiaobing Cao4Yanjun Li5Huaru Cao6Yongzhi Men7Zhejiang Provincial Key Laboratory of Chemical Utilization of Forestry Biomass, Zhejiang A & F University, Hangzhou, ChinaInstitute of Technical and Macromolecular Chemistry, RWTH Aachen University, Aachen, GermanyDepartment of Surgical Oncology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, ChinaInstitute of Technical and Macromolecular Chemistry, RWTH Aachen University, Aachen, GermanyCo-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, ChinaCo-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, ChinaZhejiang Provincial Key Laboratory of Chemical Utilization of Forestry Biomass, Zhejiang A & F University, Hangzhou, ChinaShanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaRecently, natural resources have attracted considerable interest for their applications in food security and human health problems. Traditional natural spices, such as star anise and black pepper, played important roles in the pharmaceutical and food industries due to their strong pharmacological activity, antioxidant potential and rare complications. In order to achieve biomasses from the natural product with multiple bioactivities, we developed the multistage extraction method to extract and separate various bioactive compounds from these natural plants. Our work demonstrated that various bioactive-rich extractives were achieved using steam distilled- or oxidative-extraction methods with high extraction yields and purity. Furthermore, the extractives in each step can be used not only as bioactive compounds, but also as a resource to further prepare different derivatives during the next extractive step, providing biomass-saving to a great extent. The extractives obtained with high yields and purities (>82%) were identified by 1H NMR, 13C NMR, FTIR, UV-vis, fluorescence spectroscopy, and high-performance liquid chromatography (HPLC). Moreover, these biomasses display potent antibacterial activities against some types of microorganisms such as S.aureus, S.pyogenes, E.coli, and S.typhi with a lowest MIC of 400 μg/ml for the development of antibacterial agents, significant antioxidant activity as the natural antioxidant for enhancing food shelf-life, and excellent anticancer activity that induces significant cancer cell apoptosis. This work showed the different multistage extracts from natural products, which enable them to be applied in the fields of the pharmaceutical industry and the food industry.https://www.frontiersin.org/articles/10.3389/fchem.2021.660138/fullnatural productmultistage extractionantibacterialantioxidantanticancer |
| spellingShingle | Helin Li Helin Li Xiaoyu Wu Xin Li Xiaobing Cao Yanjun Li Huaru Cao Yongzhi Men Multistage Extraction of Star Anise and Black Pepper Derivatives for Antibacterial, Antioxidant, and Anticancer Activity Frontiers in Chemistry natural product multistage extraction antibacterial antioxidant anticancer |
| title | Multistage Extraction of Star Anise and Black Pepper Derivatives for Antibacterial, Antioxidant, and Anticancer Activity |
| title_full | Multistage Extraction of Star Anise and Black Pepper Derivatives for Antibacterial, Antioxidant, and Anticancer Activity |
| title_fullStr | Multistage Extraction of Star Anise and Black Pepper Derivatives for Antibacterial, Antioxidant, and Anticancer Activity |
| title_full_unstemmed | Multistage Extraction of Star Anise and Black Pepper Derivatives for Antibacterial, Antioxidant, and Anticancer Activity |
| title_short | Multistage Extraction of Star Anise and Black Pepper Derivatives for Antibacterial, Antioxidant, and Anticancer Activity |
| title_sort | multistage extraction of star anise and black pepper derivatives for antibacterial antioxidant and anticancer activity |
| topic | natural product multistage extraction antibacterial antioxidant anticancer |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2021.660138/full |
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