Novel Fluorescence Probe toward Cu<sup>2+</sup> Based on Fluorescein Derivatives and Its Bioimaging in Cells
Copper is an important trace element that plays a crucial role in various physiological and biochemical processes in the body. The level of copper content is significantly related to many diseases, so it is very important to establish effective and sensitive methods for copper detection in vitro and...
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MDPI AG
2022-09-01
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| Series: | Biosensors |
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| author | Xin Leng Du Wang Zhaoxiang Mi Yuchen Zhang Bingqin Yang Fulin Chen |
| author_facet | Xin Leng Du Wang Zhaoxiang Mi Yuchen Zhang Bingqin Yang Fulin Chen |
| author_sort | Xin Leng |
| collection | DOAJ |
| description | Copper is an important trace element that plays a crucial role in various physiological and biochemical processes in the body. The level of copper content is significantly related to many diseases, so it is very important to establish effective and sensitive methods for copper detection in vitro and vivo. Copper-selective probes have attracted considerable interest in environmental testing and life-process research, but fewer investigations have focused on the luminescence mechanism and bioimaging for Cu<sup>2+</sup> detection. In the current study, a novel fluorescein-based A5 fluorescence probe is synthesized and characterized, and the bioimaging performance of the probe is also tested. We observed that the A5 displayed extraordinary selectivity and sensitivity properties to Cu<sup>2+</sup> in contrast to other cations in solution. The reaction between A5 and Cu<sup>2+</sup> could accelerate the ring-opening process, resulting in a new band at 525 nm during a larger pH range. A good linearity between the fluorescence intensity and concentrations of Cu<sup>2+</sup>, ranging from 0.1 to 1.5 equivalent, was observed, and the limit detection of A5 to Cu<sup>2+</sup> was 0.11 μM. In addition, the Job’s plot and mass spectrum showed that A5 complexed Cu<sup>2+</sup> in a 1:1 manner. The apparent color change in the A5–Cu<sup>2+</sup> complex under ultraviolet light at low molar concentrations revealed that A5 is a suitable probe for the detection of Cu<sup>2+</sup>. The biological test results show that the A5 probe has good biocompatibility and can be used for the cell imaging of Cu<sup>2+</sup>. |
| first_indexed | 2024-03-10T00:35:40Z |
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| id | doaj.art-b3bce673894644aaa87f2d077ac03e63 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T00:35:40Z |
| publishDate | 2022-09-01 |
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| series | Biosensors |
| spelling | doaj.art-b3bce673894644aaa87f2d077ac03e632023-11-23T15:18:14ZengMDPI AGBiosensors2079-63742022-09-0112973210.3390/bios12090732Novel Fluorescence Probe toward Cu<sup>2+</sup> Based on Fluorescein Derivatives and Its Bioimaging in CellsXin Leng0Du Wang1Zhaoxiang Mi2Yuchen Zhang3Bingqin Yang4Fulin Chen5College of Life Sciences, Northwest University, Xi’an 710069, ChinaCollege of Life Sciences, Northwest University, Xi’an 710069, ChinaCollege of Life Sciences, Northwest University, Xi’an 710069, ChinaCollege of Life Sciences, Northwest University, Xi’an 710069, ChinaCollege of Chemistry & Materials Science, Northwest University, Xi’an 710127, ChinaCollege of Life Sciences, Northwest University, Xi’an 710069, ChinaCopper is an important trace element that plays a crucial role in various physiological and biochemical processes in the body. The level of copper content is significantly related to many diseases, so it is very important to establish effective and sensitive methods for copper detection in vitro and vivo. Copper-selective probes have attracted considerable interest in environmental testing and life-process research, but fewer investigations have focused on the luminescence mechanism and bioimaging for Cu<sup>2+</sup> detection. In the current study, a novel fluorescein-based A5 fluorescence probe is synthesized and characterized, and the bioimaging performance of the probe is also tested. We observed that the A5 displayed extraordinary selectivity and sensitivity properties to Cu<sup>2+</sup> in contrast to other cations in solution. The reaction between A5 and Cu<sup>2+</sup> could accelerate the ring-opening process, resulting in a new band at 525 nm during a larger pH range. A good linearity between the fluorescence intensity and concentrations of Cu<sup>2+</sup>, ranging from 0.1 to 1.5 equivalent, was observed, and the limit detection of A5 to Cu<sup>2+</sup> was 0.11 μM. In addition, the Job’s plot and mass spectrum showed that A5 complexed Cu<sup>2+</sup> in a 1:1 manner. The apparent color change in the A5–Cu<sup>2+</sup> complex under ultraviolet light at low molar concentrations revealed that A5 is a suitable probe for the detection of Cu<sup>2+</sup>. The biological test results show that the A5 probe has good biocompatibility and can be used for the cell imaging of Cu<sup>2+</sup>.https://www.mdpi.com/2079-6374/12/9/732copper ionfluorescence probeluminescence mechanismdetection methodbioimaging |
| spellingShingle | Xin Leng Du Wang Zhaoxiang Mi Yuchen Zhang Bingqin Yang Fulin Chen Novel Fluorescence Probe toward Cu<sup>2+</sup> Based on Fluorescein Derivatives and Its Bioimaging in Cells Biosensors copper ion fluorescence probe luminescence mechanism detection method bioimaging |
| title | Novel Fluorescence Probe toward Cu<sup>2+</sup> Based on Fluorescein Derivatives and Its Bioimaging in Cells |
| title_full | Novel Fluorescence Probe toward Cu<sup>2+</sup> Based on Fluorescein Derivatives and Its Bioimaging in Cells |
| title_fullStr | Novel Fluorescence Probe toward Cu<sup>2+</sup> Based on Fluorescein Derivatives and Its Bioimaging in Cells |
| title_full_unstemmed | Novel Fluorescence Probe toward Cu<sup>2+</sup> Based on Fluorescein Derivatives and Its Bioimaging in Cells |
| title_short | Novel Fluorescence Probe toward Cu<sup>2+</sup> Based on Fluorescein Derivatives and Its Bioimaging in Cells |
| title_sort | novel fluorescence probe toward cu sup 2 sup based on fluorescein derivatives and its bioimaging in cells |
| topic | copper ion fluorescence probe luminescence mechanism detection method bioimaging |
| url | https://www.mdpi.com/2079-6374/12/9/732 |
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