Ultrastructural and Immunofluorescent Analysis of Lithium Effects on Autophagy in Hepatocellular Carcinoma Cells
Background: Hepatocellular carcinoma (HCC) is one of the most malignant cancers worldwide. The role of autophagy in the НСС development and progression is controversial and are still not well known. It has been shown that lithium induces autophagy and apoptosis, affects on proliferation and survival...
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| Format: | Article |
| Language: | English |
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West Asia Organization for Cancer Prevention
2018-11-01
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| Series: | Asian Pacific Journal of Cancer Biology |
| Subjects: | |
| Online Access: | http://www.waocp.com/journal/index.php/apjcb/article/view/240 |
| _version_ | 1797341167350710272 |
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| author | Iuliia Taskaeva Nataliya Bgatova |
| author_facet | Iuliia Taskaeva Nataliya Bgatova |
| author_sort | Iuliia Taskaeva |
| collection | DOAJ |
| description | Background: Hepatocellular carcinoma (HCC) is one of the most malignant cancers worldwide. The role of autophagy in the НСС development and progression is controversial and are still not well known. It has been shown that lithium induces autophagy and apoptosis, affects on proliferation and survival of various human malignancies. In this study we estimated lithium`s effects on autophagy in HCC in vivo.
Materials and methods: Subcellular components and autophagic vacuoles were analyzed by transmission electron microscopy. The number of cells with LC3 and LAMP1 punctate was analyzed by double-immunofluorescence staining. Data are presented as mean ± standard deviation (SD). Mann–Whitney nonparametric tests were used to assess statistically significant differences at P < 0.05.
Results: Lithium carbonate increased the numerical and volume density of autophagic vacuoles and autophagy marker LC3 beta. Therefore, lithium carbonate promotes enlarged autophagic vacuoles formation in HCC cells in vivo.
Conclusion: Extended and unresolved autophagy induces cancer cell death by stimulating autophagic cell death. Thus, lithium-mediated autophagy can be an attractive approach in HCC chemotherapy. |
| first_indexed | 2024-03-08T10:13:55Z |
| format | Article |
| id | doaj.art-b3c2c34d2035492bbfe7f4bb47ab6338 |
| institution | Directory Open Access Journal |
| issn | 2538-4635 |
| language | English |
| last_indexed | 2024-03-08T10:13:55Z |
| publishDate | 2018-11-01 |
| publisher | West Asia Organization for Cancer Prevention |
| record_format | Article |
| series | Asian Pacific Journal of Cancer Biology |
| spelling | doaj.art-b3c2c34d2035492bbfe7f4bb47ab63382024-01-29T05:58:58ZengWest Asia Organization for Cancer PreventionAsian Pacific Journal of Cancer Biology2538-46352018-11-0133838710.31557/apjcb.2018.3.3.83-87240Ultrastructural and Immunofluorescent Analysis of Lithium Effects on Autophagy in Hepatocellular Carcinoma CellsIuliia Taskaeva0Nataliya Bgatova1Researcher, laboratory of Ultrastructural research, Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences Timakova str., 2630060, Novosibirsk, RussiaDoctor of biological sciences, professor, Head of laboratory of Ultrastructural research, Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences Timakova str., 2630060, Novosibirsk, Russia.Background: Hepatocellular carcinoma (HCC) is one of the most malignant cancers worldwide. The role of autophagy in the НСС development and progression is controversial and are still not well known. It has been shown that lithium induces autophagy and apoptosis, affects on proliferation and survival of various human malignancies. In this study we estimated lithium`s effects on autophagy in HCC in vivo. Materials and methods: Subcellular components and autophagic vacuoles were analyzed by transmission electron microscopy. The number of cells with LC3 and LAMP1 punctate was analyzed by double-immunofluorescence staining. Data are presented as mean ± standard deviation (SD). Mann–Whitney nonparametric tests were used to assess statistically significant differences at P < 0.05. Results: Lithium carbonate increased the numerical and volume density of autophagic vacuoles and autophagy marker LC3 beta. Therefore, lithium carbonate promotes enlarged autophagic vacuoles formation in HCC cells in vivo. Conclusion: Extended and unresolved autophagy induces cancer cell death by stimulating autophagic cell death. Thus, lithium-mediated autophagy can be an attractive approach in HCC chemotherapy.http://www.waocp.com/journal/index.php/apjcb/article/view/240hepatocellular carcinoma- lithium carbonate- autophagy- electron microscopy- immunofluorescence |
| spellingShingle | Iuliia Taskaeva Nataliya Bgatova Ultrastructural and Immunofluorescent Analysis of Lithium Effects on Autophagy in Hepatocellular Carcinoma Cells Asian Pacific Journal of Cancer Biology hepatocellular carcinoma- lithium carbonate- autophagy- electron microscopy- immunofluorescence |
| title | Ultrastructural and Immunofluorescent Analysis of Lithium Effects on Autophagy in Hepatocellular Carcinoma Cells |
| title_full | Ultrastructural and Immunofluorescent Analysis of Lithium Effects on Autophagy in Hepatocellular Carcinoma Cells |
| title_fullStr | Ultrastructural and Immunofluorescent Analysis of Lithium Effects on Autophagy in Hepatocellular Carcinoma Cells |
| title_full_unstemmed | Ultrastructural and Immunofluorescent Analysis of Lithium Effects on Autophagy in Hepatocellular Carcinoma Cells |
| title_short | Ultrastructural and Immunofluorescent Analysis of Lithium Effects on Autophagy in Hepatocellular Carcinoma Cells |
| title_sort | ultrastructural and immunofluorescent analysis of lithium effects on autophagy in hepatocellular carcinoma cells |
| topic | hepatocellular carcinoma- lithium carbonate- autophagy- electron microscopy- immunofluorescence |
| url | http://www.waocp.com/journal/index.php/apjcb/article/view/240 |
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