| Summary: | The emergence of drug resistant microbes over recent decades represents one of the greatest threats to human health; the resilience of many of these organisms can be attributed to their ability to produce biofilms. Natural products have played a crucial role in drug discovery, with microbial natural products in particular proving a rich and diverse source of antimicrobial agents. During antimicrobial activity screening, the strain <i>Pseudomonas mosselii</i> P33 was found to inhibit the growth of multiple pathogens. Following chemical investigation of this strain, pseudopyronines A-C were isolated as the main active principles, with all three pseudopyronines showing outstanding activity against <i>Staphylococcus aureus</i>. The analogue pseudopyronine C, which has not been well-characterized previously, displayed sub-micromolar activity against <i>S. aureus</i>, <i>Staphylococcus epidermidis</i> and <i>Pseudomonas aeruginosa</i>. Moreover, the inhibitory abilities of the pseudopyronines against the biofilms of <i>S. aureus</i> were further studied. The results indicated all three pseudopyronines could directly reduce the growth of biofilm in both adhesion stage and maturation stage, displaying significant activity at micromolar concentrations.
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