<i>Fusarium</i> Wilt Invasion Results in a Strong Impact on Strawberry Microbiomes
Plant-endophytic microbes affect plant growth, development, nutrition, and resistance to pathogens. However, how endophytic microbial communities change in different strawberry plant compartments after <i>Fusarium</i> pathogen infection has remained elusive. In this study, 16S and intern...
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MDPI AG
2023-12-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/24/4153 |
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| author | Hongjun Yang Xu Zhang Xiaohong Qiu Jiajia Chen Yuanhua Wang Geng Zhang Sizhen Jia Xiangqi Shen Wenwu Ye Zhiming Yan |
| author_facet | Hongjun Yang Xu Zhang Xiaohong Qiu Jiajia Chen Yuanhua Wang Geng Zhang Sizhen Jia Xiangqi Shen Wenwu Ye Zhiming Yan |
| author_sort | Hongjun Yang |
| collection | DOAJ |
| description | Plant-endophytic microbes affect plant growth, development, nutrition, and resistance to pathogens. However, how endophytic microbial communities change in different strawberry plant compartments after <i>Fusarium</i> pathogen infection has remained elusive. In this study, 16S and internal transcribed spacer rRNA amplicon sequencing were used to systematically investigate changes in the bacterial and fungal diversity and composition in the endophytic compartments (roots, stems, and leaves) of healthy strawberries and strawberries with <i>Fusarium</i> wilt, respectively. The analysis of the diversity, structure, and composition of the bacterial and fungal communities revealed a strong effect of pathogen invasion on the endophytic communities. The bacterial and fungal community diversity was lower in the <i>Fusarium</i>-infected endophytic compartments than in the healthy samples. The relative abundance of certain bacterial and fungal genera also changed after <i>Fusarium</i> wilt infection. The relative abundance of the beneficial bacterial genera <i>Bacillus</i>, <i>Bradyrhizobium</i>, <i>Methylophilus</i>, <i>Sphingobium</i>, <i>Lactobacillus</i>, and <i>Streptomyces</i>, as well as fungal genera <i>Acremonium</i>, <i>Penicillium</i>, <i>Talaromyces</i>, and <i>Trichoderma</i>, were higher in the healthy samples than in the <i>Fusarium</i> wilt samples. The relative abundance of <i>Fusarium</i> in the infected samples was significantly higher than that in the healthy samples, consistent with the field observations and culture isolation results for strawberry wilt. Our findings provide a theoretical basis for the isolation, identification, and control of strawberry wilt disease. |
| first_indexed | 2024-03-08T18:35:05Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-08T18:35:05Z |
| publishDate | 2023-12-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-b3c6cb03aaf249cc88fb245097ac12182023-12-29T15:47:31ZengMDPI AGPlants2223-77472023-12-011224415310.3390/plants12244153<i>Fusarium</i> Wilt Invasion Results in a Strong Impact on Strawberry MicrobiomesHongjun Yang0Xu Zhang1Xiaohong Qiu2Jiajia Chen3Yuanhua Wang4Geng Zhang5Sizhen Jia6Xiangqi Shen7Wenwu Ye8Zhiming Yan9College of Agronomy and Horticulture, Jiangsu Vocational College of Agriculture and Forestry, Zhenjiang 212400, ChinaCollege of Agronomy and Horticulture, Jiangsu Vocational College of Agriculture and Forestry, Zhenjiang 212400, ChinaCollege of Agronomy and Horticulture, Jiangsu Vocational College of Agriculture and Forestry, Zhenjiang 212400, ChinaCollege of Landscape Architecture, Jiangsu Vocational College of Agriculture and Forestry, Zhenjiang 212400, ChinaCollege of Agronomy and Horticulture, Jiangsu Vocational College of Agriculture and Forestry, Zhenjiang 212400, ChinaCollege of Agronomy and Horticulture, Jiangsu Vocational College of Agriculture and Forestry, Zhenjiang 212400, ChinaCollege of Agronomy and Horticulture, Jiangsu Vocational College of Agriculture and Forestry, Zhenjiang 212400, ChinaCollege of Agronomy and Horticulture, Jiangsu Vocational College of Agriculture and Forestry, Zhenjiang 212400, ChinaKey Laboratory of Plant Immunity, Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Agronomy and Horticulture, Jiangsu Vocational College of Agriculture and Forestry, Zhenjiang 212400, ChinaPlant-endophytic microbes affect plant growth, development, nutrition, and resistance to pathogens. However, how endophytic microbial communities change in different strawberry plant compartments after <i>Fusarium</i> pathogen infection has remained elusive. In this study, 16S and internal transcribed spacer rRNA amplicon sequencing were used to systematically investigate changes in the bacterial and fungal diversity and composition in the endophytic compartments (roots, stems, and leaves) of healthy strawberries and strawberries with <i>Fusarium</i> wilt, respectively. The analysis of the diversity, structure, and composition of the bacterial and fungal communities revealed a strong effect of pathogen invasion on the endophytic communities. The bacterial and fungal community diversity was lower in the <i>Fusarium</i>-infected endophytic compartments than in the healthy samples. The relative abundance of certain bacterial and fungal genera also changed after <i>Fusarium</i> wilt infection. The relative abundance of the beneficial bacterial genera <i>Bacillus</i>, <i>Bradyrhizobium</i>, <i>Methylophilus</i>, <i>Sphingobium</i>, <i>Lactobacillus</i>, and <i>Streptomyces</i>, as well as fungal genera <i>Acremonium</i>, <i>Penicillium</i>, <i>Talaromyces</i>, and <i>Trichoderma</i>, were higher in the healthy samples than in the <i>Fusarium</i> wilt samples. The relative abundance of <i>Fusarium</i> in the infected samples was significantly higher than that in the healthy samples, consistent with the field observations and culture isolation results for strawberry wilt. Our findings provide a theoretical basis for the isolation, identification, and control of strawberry wilt disease.https://www.mdpi.com/2223-7747/12/24/4153microbiomestrawberry <i>Fusarium</i> wilt diseasefungal communitybacterial communityplant pathogen |
| spellingShingle | Hongjun Yang Xu Zhang Xiaohong Qiu Jiajia Chen Yuanhua Wang Geng Zhang Sizhen Jia Xiangqi Shen Wenwu Ye Zhiming Yan <i>Fusarium</i> Wilt Invasion Results in a Strong Impact on Strawberry Microbiomes Plants microbiome strawberry <i>Fusarium</i> wilt disease fungal community bacterial community plant pathogen |
| title | <i>Fusarium</i> Wilt Invasion Results in a Strong Impact on Strawberry Microbiomes |
| title_full | <i>Fusarium</i> Wilt Invasion Results in a Strong Impact on Strawberry Microbiomes |
| title_fullStr | <i>Fusarium</i> Wilt Invasion Results in a Strong Impact on Strawberry Microbiomes |
| title_full_unstemmed | <i>Fusarium</i> Wilt Invasion Results in a Strong Impact on Strawberry Microbiomes |
| title_short | <i>Fusarium</i> Wilt Invasion Results in a Strong Impact on Strawberry Microbiomes |
| title_sort | i fusarium i wilt invasion results in a strong impact on strawberry microbiomes |
| topic | microbiome strawberry <i>Fusarium</i> wilt disease fungal community bacterial community plant pathogen |
| url | https://www.mdpi.com/2223-7747/12/24/4153 |
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