Measurement of changes in urinary neopterin and total neopterin in body builders using SCX HPLC
Body building is a sport where ultrastructual damage to muscle fibres aids the development of dense muscle layers. Using a new strong cation exchange (SCX) based chromatography technique to measure neopterin and 7,8-dihydroneopterin, we investigated whether this muscle damage caused increased levels...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2014-07-01
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| Series: | Pteridines |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/pteridines-2014-0003 |
| _version_ | 1818729580216713216 |
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| author | Lindsay Angus Janmale Tejraj Draper Nick Gieseg Steven P. |
| author_facet | Lindsay Angus Janmale Tejraj Draper Nick Gieseg Steven P. |
| author_sort | Lindsay Angus |
| collection | DOAJ |
| description | Body building is a sport where ultrastructual damage to muscle fibres aids the development of dense muscle layers. Using a new strong cation exchange (SCX) based chromatography technique to measure neopterin and 7,8-dihydroneopterin, we investigated whether this muscle damage caused increased levels of inflammation. Urine samples were collected over eight consecutive mornings from 10 natural competitive body builders. Samples were analysed using SCX high performance liquid chromatography (HPLC) with urine volume corrected for creatinine and specific gravity (SG). The majority of subjects showed large changes in both neopterin and total neopterin (7,8-dihydroneopterin+neopterin) levels, though the mean data for the group showed no significant change over the week. There was no evidence of the high intensity resistance training causing an accumulation of inflammation as the values for all the body builders returned to close to the starting values after 2 days rest. The SCX analysis had an intra-specific viability of 3.04% and the inter-specific viability was 5.42%. Urine volume correction with SG was found to give the same values as using creatinine. Creatinine and specific gravity are both reliable methods for correcting for urine volume while SCX HPLC provides a new means of measuring urinary neopterin and total neopterin. |
| first_indexed | 2024-12-17T22:48:09Z |
| format | Article |
| id | doaj.art-b3c73d3765ba42a58ff6f87399e7c9da |
| institution | Directory Open Access Journal |
| issn | 0933-4807 2195-4720 |
| language | English |
| last_indexed | 2024-12-17T22:48:09Z |
| publishDate | 2014-07-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Pteridines |
| spelling | doaj.art-b3c73d3765ba42a58ff6f87399e7c9da2022-12-21T21:29:45ZengDe GruyterPteridines0933-48072195-47202014-07-01252536310.1515/pteridines-2014-0003Measurement of changes in urinary neopterin and total neopterin in body builders using SCX HPLCLindsay Angus0Janmale Tejraj1Draper Nick2Gieseg Steven P.3Free Radical Biochemistry Laboratory, School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch 8140, New ZealandFree Radical Biochemistry Laboratory, School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch 8140, New ZealandSchool of Sport and Physical Education, University of Canterbury, Private Bag 4800, Christchruch 8140, New ZealandFree Radical Biochemistry Laboratory, School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch 8140, New ZealandBody building is a sport where ultrastructual damage to muscle fibres aids the development of dense muscle layers. Using a new strong cation exchange (SCX) based chromatography technique to measure neopterin and 7,8-dihydroneopterin, we investigated whether this muscle damage caused increased levels of inflammation. Urine samples were collected over eight consecutive mornings from 10 natural competitive body builders. Samples were analysed using SCX high performance liquid chromatography (HPLC) with urine volume corrected for creatinine and specific gravity (SG). The majority of subjects showed large changes in both neopterin and total neopterin (7,8-dihydroneopterin+neopterin) levels, though the mean data for the group showed no significant change over the week. There was no evidence of the high intensity resistance training causing an accumulation of inflammation as the values for all the body builders returned to close to the starting values after 2 days rest. The SCX analysis had an intra-specific viability of 3.04% and the inter-specific viability was 5.42%. Urine volume correction with SG was found to give the same values as using creatinine. Creatinine and specific gravity are both reliable methods for correcting for urine volume while SCX HPLC provides a new means of measuring urinary neopterin and total neopterin.https://doi.org/10.1515/pteridines-2014-0003body buildingchromatographycreatinineneopterinspecific gravityurine |
| spellingShingle | Lindsay Angus Janmale Tejraj Draper Nick Gieseg Steven P. Measurement of changes in urinary neopterin and total neopterin in body builders using SCX HPLC Pteridines body building chromatography creatinine neopterin specific gravity urine |
| title | Measurement of changes in urinary neopterin and total neopterin in body builders using SCX HPLC |
| title_full | Measurement of changes in urinary neopterin and total neopterin in body builders using SCX HPLC |
| title_fullStr | Measurement of changes in urinary neopterin and total neopterin in body builders using SCX HPLC |
| title_full_unstemmed | Measurement of changes in urinary neopterin and total neopterin in body builders using SCX HPLC |
| title_short | Measurement of changes in urinary neopterin and total neopterin in body builders using SCX HPLC |
| title_sort | measurement of changes in urinary neopterin and total neopterin in body builders using scx hplc |
| topic | body building chromatography creatinine neopterin specific gravity urine |
| url | https://doi.org/10.1515/pteridines-2014-0003 |
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