Effects of dietary glutathione on growth performance, muscle quality and lipid metabolism of hybrid crucian carp (Carassius auratus cuvieri ♀ × Carassius auratus red var. ♂) fed a high-fat diet
A 60-day feeding trial was conducted to investigate the influence of glutathione (GSH) on growth performance, lipid metabolism and muscle quality of hybrid crucian carp (initial body weight: 15.03 ± 0.11 g) fed a high-fat diet (HFD). Five experimental diets were prepared in this study: the control d...
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KeAi Communications Co. Ltd.
2023-09-01
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| Series: | Reproduction and Breeding |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266707122300025X |
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| author | Zhongtian Tang Chunyan Li Gang Xu Qiang Zhao Zehong Wei Shaojun Liu |
| author_facet | Zhongtian Tang Chunyan Li Gang Xu Qiang Zhao Zehong Wei Shaojun Liu |
| author_sort | Zhongtian Tang |
| collection | DOAJ |
| description | A 60-day feeding trial was conducted to investigate the influence of glutathione (GSH) on growth performance, lipid metabolism and muscle quality of hybrid crucian carp (initial body weight: 15.03 ± 0.11 g) fed a high-fat diet (HFD). Five experimental diets were prepared in this study: the control diet (6.17% fat), HFD (12.37% fat), and HFD with 200 mg/kg, 400 mg/kg and 600 mg/kg GSH supplementation, respectively. The results indicated that feeding HFD to hybrid crucian carp remarkably reduced weight gain rate (WGR), specific growth rate (SGR), total antioxidant capacity (T-AOC) and total superoxide dismutase (T-SOD) activities of serum and liver, glutathione peroxidase (GSH-Px) and glutathione reductase (GR) activities of liver, but significantly increased malondialdehyde (MDA) content of liver compared to the control group. After adding 200–600 mg/kg GSH to HFD, the WGR, SGR, as well as serum and liver T-AOC activity of hybrid crucian carp increased significantly. Moreover, the supplementation of 400 mg/kg GSH significantly increased the activities of T-SOD, catalase (CAT) of serum and liver, as well as the GSH-Px, glutathione-S-transferase (GST) and GR of liver, but decreased MDA content of serum and liver compared to the HFD. The HFD significantly increased muscle crude lipid, polyunsaturated fatty acids (PUFA) contents and fiber diameter, but decreased chewiness, gumminess, hardness and fiber density compared to the control group. The addition of 400–600 mg/kg GSH significantly increased muscle chewiness, gumminess, hardness, and decreased muscle fiber diameter of HFD-fed hybrid crucian carp. Meanwhile, the addition of 200–600 mg/kg GSH significantly reduced the mono-unsaturated fatty acids (MUFA) content and increased the PUFA content of muscle compared to the HFD. The HFD significantly up-regulated the expression of lipid synthesis genes (dgat2 and srebp1) and down-regulated the expression of lipolysis genes (lpl, hsl and acox1) of liver compared to the control group. The addition of 400–600 mg/kg GSH significantly decreased the expression of lipid synthesis genes (dgat2 and srebp1) and increased the expression of lipolysis genes (hsl, lpl, acox1 and pparα) of HFD-fed hybrid crucian carp. This study demonstrated that GSH effectively ameliorated the decline of growth performance and muscle quality of hybrid crucian carp caused by HFD, and reduced the risk of liver lipid deposition of hybrid crucian carp by regulating the expression of genes related to liver lipid metabolism. |
| first_indexed | 2024-03-12T15:30:48Z |
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| language | English |
| last_indexed | 2024-03-12T15:30:48Z |
| publishDate | 2023-09-01 |
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| series | Reproduction and Breeding |
| spelling | doaj.art-b3cae6c58d80446b9836a3932bd9620d2023-08-10T04:35:03ZengKeAi Communications Co. Ltd.Reproduction and Breeding2667-07122023-09-01338998Effects of dietary glutathione on growth performance, muscle quality and lipid metabolism of hybrid crucian carp (Carassius auratus cuvieri ♀ × Carassius auratus red var. ♂) fed a high-fat dietZhongtian Tang0Chunyan Li1Gang Xu2Qiang Zhao3Zehong Wei4Shaojun Liu5State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, 410081, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, 410081, ChinaHunan Flag Bio-Tech Co. Ltd., Changsha, 410100, ChinaHunan Flag Bio-Tech Co. Ltd., Changsha, 410100, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, 410081, China; Corresponding author. State Key Laboratory of Developmental Biology of Freshwater Fish College of Life Sciences, Hunan Normal University 36 Lushan Road, Changsha, 410081, PR China.State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, 410081, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, PR ChinaA 60-day feeding trial was conducted to investigate the influence of glutathione (GSH) on growth performance, lipid metabolism and muscle quality of hybrid crucian carp (initial body weight: 15.03 ± 0.11 g) fed a high-fat diet (HFD). Five experimental diets were prepared in this study: the control diet (6.17% fat), HFD (12.37% fat), and HFD with 200 mg/kg, 400 mg/kg and 600 mg/kg GSH supplementation, respectively. The results indicated that feeding HFD to hybrid crucian carp remarkably reduced weight gain rate (WGR), specific growth rate (SGR), total antioxidant capacity (T-AOC) and total superoxide dismutase (T-SOD) activities of serum and liver, glutathione peroxidase (GSH-Px) and glutathione reductase (GR) activities of liver, but significantly increased malondialdehyde (MDA) content of liver compared to the control group. After adding 200–600 mg/kg GSH to HFD, the WGR, SGR, as well as serum and liver T-AOC activity of hybrid crucian carp increased significantly. Moreover, the supplementation of 400 mg/kg GSH significantly increased the activities of T-SOD, catalase (CAT) of serum and liver, as well as the GSH-Px, glutathione-S-transferase (GST) and GR of liver, but decreased MDA content of serum and liver compared to the HFD. The HFD significantly increased muscle crude lipid, polyunsaturated fatty acids (PUFA) contents and fiber diameter, but decreased chewiness, gumminess, hardness and fiber density compared to the control group. The addition of 400–600 mg/kg GSH significantly increased muscle chewiness, gumminess, hardness, and decreased muscle fiber diameter of HFD-fed hybrid crucian carp. Meanwhile, the addition of 200–600 mg/kg GSH significantly reduced the mono-unsaturated fatty acids (MUFA) content and increased the PUFA content of muscle compared to the HFD. The HFD significantly up-regulated the expression of lipid synthesis genes (dgat2 and srebp1) and down-regulated the expression of lipolysis genes (lpl, hsl and acox1) of liver compared to the control group. The addition of 400–600 mg/kg GSH significantly decreased the expression of lipid synthesis genes (dgat2 and srebp1) and increased the expression of lipolysis genes (hsl, lpl, acox1 and pparα) of HFD-fed hybrid crucian carp. This study demonstrated that GSH effectively ameliorated the decline of growth performance and muscle quality of hybrid crucian carp caused by HFD, and reduced the risk of liver lipid deposition of hybrid crucian carp by regulating the expression of genes related to liver lipid metabolism.http://www.sciencedirect.com/science/article/pii/S266707122300025XGlutathioneHybrid crucian carpGrowth performanceMuscle qualityLipid metabolism |
| spellingShingle | Zhongtian Tang Chunyan Li Gang Xu Qiang Zhao Zehong Wei Shaojun Liu Effects of dietary glutathione on growth performance, muscle quality and lipid metabolism of hybrid crucian carp (Carassius auratus cuvieri ♀ × Carassius auratus red var. ♂) fed a high-fat diet Reproduction and Breeding Glutathione Hybrid crucian carp Growth performance Muscle quality Lipid metabolism |
| title | Effects of dietary glutathione on growth performance, muscle quality and lipid metabolism of hybrid crucian carp (Carassius auratus cuvieri ♀ × Carassius auratus red var. ♂) fed a high-fat diet |
| title_full | Effects of dietary glutathione on growth performance, muscle quality and lipid metabolism of hybrid crucian carp (Carassius auratus cuvieri ♀ × Carassius auratus red var. ♂) fed a high-fat diet |
| title_fullStr | Effects of dietary glutathione on growth performance, muscle quality and lipid metabolism of hybrid crucian carp (Carassius auratus cuvieri ♀ × Carassius auratus red var. ♂) fed a high-fat diet |
| title_full_unstemmed | Effects of dietary glutathione on growth performance, muscle quality and lipid metabolism of hybrid crucian carp (Carassius auratus cuvieri ♀ × Carassius auratus red var. ♂) fed a high-fat diet |
| title_short | Effects of dietary glutathione on growth performance, muscle quality and lipid metabolism of hybrid crucian carp (Carassius auratus cuvieri ♀ × Carassius auratus red var. ♂) fed a high-fat diet |
| title_sort | effects of dietary glutathione on growth performance muscle quality and lipid metabolism of hybrid crucian carp carassius auratus cuvieri ♀ carassius auratus red var ♂ fed a high fat diet |
| topic | Glutathione Hybrid crucian carp Growth performance Muscle quality Lipid metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S266707122300025X |
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