Trapping, characterization and reactions of biocolloids in a salinity gradient
The properties of soft matter nanoparticles like exosomes are interesting for drug delivery and diagnostics applications. However, the simultaneous characterization of multiple properties, e.g., size and zeta potential, can only be done serially and is highly sensitive to the purification prior to c...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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EDP Sciences
2022-01-01
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| Series: | EPJ Web of Conferences |
| Online Access: | https://www.epj-conferences.org/articles/epjconf/pdf/2022/10/epjconf_eosam2022_12005.pdf |
| _version_ | 1797990526134255616 |
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| author | Rasmussen Martin K. Pedersen Jonas N. Marie Rodolphe |
| author_facet | Rasmussen Martin K. Pedersen Jonas N. Marie Rodolphe |
| author_sort | Rasmussen Martin K. |
| collection | DOAJ |
| description | The properties of soft matter nanoparticles like exosomes are interesting for drug delivery and diagnostics applications. However, the simultaneous characterization of multiple properties, e.g., size and zeta potential, can only be done serially and is highly sensitive to the purification prior to characterization. Here we show how a salt gradient established in a nanofluidic channel induces opposing transport of particles and liquid that trap the particles. Particles are thus accumulated in the trap. We show how optical microscopy images of the particle positions in the salinity gradient provide a measurement of the size and surface charge. We demonstrate the method on a sample of exosomes and on individual particles. Finally, we show how biomolecular reactions at the surface of the nanoparticle can be detected from the optical microscopy analysis of the particles’ trapping position. |
| first_indexed | 2024-04-11T08:37:54Z |
| format | Article |
| id | doaj.art-b3cc9e83748049009a292af7c2ed7a52 |
| institution | Directory Open Access Journal |
| issn | 2100-014X |
| language | English |
| last_indexed | 2024-04-11T08:37:54Z |
| publishDate | 2022-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | EPJ Web of Conferences |
| spelling | doaj.art-b3cc9e83748049009a292af7c2ed7a522022-12-22T04:34:17ZengEDP SciencesEPJ Web of Conferences2100-014X2022-01-012661200510.1051/epjconf/202226612005epjconf_eosam2022_12005Trapping, characterization and reactions of biocolloids in a salinity gradientRasmussen Martin K.0Pedersen Jonas N.1Marie Rodolphe2Department of Health Technology, Technical University of DenmarkDepartment of Health Technology, Technical University of DenmarkDepartment of Health Technology, Technical University of DenmarkThe properties of soft matter nanoparticles like exosomes are interesting for drug delivery and diagnostics applications. However, the simultaneous characterization of multiple properties, e.g., size and zeta potential, can only be done serially and is highly sensitive to the purification prior to characterization. Here we show how a salt gradient established in a nanofluidic channel induces opposing transport of particles and liquid that trap the particles. Particles are thus accumulated in the trap. We show how optical microscopy images of the particle positions in the salinity gradient provide a measurement of the size and surface charge. We demonstrate the method on a sample of exosomes and on individual particles. Finally, we show how biomolecular reactions at the surface of the nanoparticle can be detected from the optical microscopy analysis of the particles’ trapping position.https://www.epj-conferences.org/articles/epjconf/pdf/2022/10/epjconf_eosam2022_12005.pdf |
| spellingShingle | Rasmussen Martin K. Pedersen Jonas N. Marie Rodolphe Trapping, characterization and reactions of biocolloids in a salinity gradient EPJ Web of Conferences |
| title | Trapping, characterization and reactions of biocolloids in a salinity gradient |
| title_full | Trapping, characterization and reactions of biocolloids in a salinity gradient |
| title_fullStr | Trapping, characterization and reactions of biocolloids in a salinity gradient |
| title_full_unstemmed | Trapping, characterization and reactions of biocolloids in a salinity gradient |
| title_short | Trapping, characterization and reactions of biocolloids in a salinity gradient |
| title_sort | trapping characterization and reactions of biocolloids in a salinity gradient |
| url | https://www.epj-conferences.org/articles/epjconf/pdf/2022/10/epjconf_eosam2022_12005.pdf |
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