Effects of the Antimicrobial Peptide Mastoparan X on the Performance, Permeability and Microbiota Populations of Broiler Chickens
Restrictions on antibiotics are driving the search for alternative feed additives to promote gastrointestinal health and development in broiler chicken production. Proteins including antimicrobial peptides can potentially be applied as alternatives to antibiotics and are one of the most promising al...
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MDPI AG
2022-12-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/24/3462 |
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| author | Chunling Zhu Yilin Bai Xiaojing Xia Man Zhang Xilong Wu Yundi Wu Yueyu Bai Shanqin Liu Gaiping Zhang Jianhe Hu Hanna Fotina Lei Wang Xueqin Zhao |
| author_facet | Chunling Zhu Yilin Bai Xiaojing Xia Man Zhang Xilong Wu Yundi Wu Yueyu Bai Shanqin Liu Gaiping Zhang Jianhe Hu Hanna Fotina Lei Wang Xueqin Zhao |
| author_sort | Chunling Zhu |
| collection | DOAJ |
| description | Restrictions on antibiotics are driving the search for alternative feed additives to promote gastrointestinal health and development in broiler chicken production. Proteins including antimicrobial peptides can potentially be applied as alternatives to antibiotics and are one of the most promising alternatives. We investigated whether the addition of MPX to the diet affects the production performance, immune function and the intestinal flora of the caecal contents of broiler chickens. One hundred one-day-old chickens were randomly divided into two groups: control (basal diet) and MPX (20 mg/kg) added to the basal diet. The results indicated that dietary supplementation with MPX improved the performance and immune organ index, decreased the feed conversion ratio, increased the villus length, maintained the normal intestinal morphology and reduced the <i>IL-6</i> and <i>LITNF</i> mRNA expression levels of inflammation-related genes. In addition, MPX increased the mRNA expression of the digestive enzymes <i>FABP2</i> and <i>SLC2A5/GLUT5</i> and the tight junction proteins <i>ZO-1</i>, <i>Claudin-1</i>, <i>Occludin</i>, <i>JAM-2</i> and <i>MUC2</i>, maintained the intestinal permeability and regulated the intestinal morphology. Moreover, MPX increased the <i>CAT</i>, <i>HMOX1</i> and <i>SOD1</i> mRNA expression levels of the antioxidant genes. Furthermore, a 16S rRNA microflora analysis indicated that the abundance of <i>Lactobacillus</i> and <i>Lactococcus</i> in the cecum was increased after addition of MPX at 14 d and 28 d. This study explored the feasibility of using antimicrobial peptides as novel feed additives for broiler chickens and provides a theoretical basis for their application in livestock. |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T17:25:12Z |
| publishDate | 2022-12-01 |
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| series | Animals |
| spelling | doaj.art-b3cf3217d0274781911537f83222f2cd2023-11-24T12:50:08ZengMDPI AGAnimals2076-26152022-12-011224346210.3390/ani12243462Effects of the Antimicrobial Peptide Mastoparan X on the Performance, Permeability and Microbiota Populations of Broiler ChickensChunling Zhu0Yilin Bai1Xiaojing Xia2Man Zhang3Xilong Wu4Yundi Wu5Yueyu Bai6Shanqin Liu7Gaiping Zhang8Jianhe Hu9Hanna Fotina10Lei Wang11Xueqin Zhao12College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, ChinaCollege of Fisheries Henan Normal University, Henan Normal University, Xinxiang 453003, ChinaState Key Laboratory of Marine Resource Utilization in the South China Sea, School of Biomedical Engineering, Hainan University, Haikou 570100, ChinaState Key Laboratory of Marine Resource Utilization in the South China Sea, School of Biomedical Engineering, Hainan University, Haikou 570100, ChinaCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, ChinaCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, ChinaCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, ChinaCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, ChinaFaculty of Veterinary Medicine, Sumy National Agrarian University, 40021 Sumy, UkraineCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, ChinaCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, ChinaRestrictions on antibiotics are driving the search for alternative feed additives to promote gastrointestinal health and development in broiler chicken production. Proteins including antimicrobial peptides can potentially be applied as alternatives to antibiotics and are one of the most promising alternatives. We investigated whether the addition of MPX to the diet affects the production performance, immune function and the intestinal flora of the caecal contents of broiler chickens. One hundred one-day-old chickens were randomly divided into two groups: control (basal diet) and MPX (20 mg/kg) added to the basal diet. The results indicated that dietary supplementation with MPX improved the performance and immune organ index, decreased the feed conversion ratio, increased the villus length, maintained the normal intestinal morphology and reduced the <i>IL-6</i> and <i>LITNF</i> mRNA expression levels of inflammation-related genes. In addition, MPX increased the mRNA expression of the digestive enzymes <i>FABP2</i> and <i>SLC2A5/GLUT5</i> and the tight junction proteins <i>ZO-1</i>, <i>Claudin-1</i>, <i>Occludin</i>, <i>JAM-2</i> and <i>MUC2</i>, maintained the intestinal permeability and regulated the intestinal morphology. Moreover, MPX increased the <i>CAT</i>, <i>HMOX1</i> and <i>SOD1</i> mRNA expression levels of the antioxidant genes. Furthermore, a 16S rRNA microflora analysis indicated that the abundance of <i>Lactobacillus</i> and <i>Lactococcus</i> in the cecum was increased after addition of MPX at 14 d and 28 d. This study explored the feasibility of using antimicrobial peptides as novel feed additives for broiler chickens and provides a theoretical basis for their application in livestock.https://www.mdpi.com/2076-2615/12/24/3462antimicrobial peptide mastoparan Xbroiler chickensperformanceintestinal healthimmunity |
| spellingShingle | Chunling Zhu Yilin Bai Xiaojing Xia Man Zhang Xilong Wu Yundi Wu Yueyu Bai Shanqin Liu Gaiping Zhang Jianhe Hu Hanna Fotina Lei Wang Xueqin Zhao Effects of the Antimicrobial Peptide Mastoparan X on the Performance, Permeability and Microbiota Populations of Broiler Chickens Animals antimicrobial peptide mastoparan X broiler chickens performance intestinal health immunity |
| title | Effects of the Antimicrobial Peptide Mastoparan X on the Performance, Permeability and Microbiota Populations of Broiler Chickens |
| title_full | Effects of the Antimicrobial Peptide Mastoparan X on the Performance, Permeability and Microbiota Populations of Broiler Chickens |
| title_fullStr | Effects of the Antimicrobial Peptide Mastoparan X on the Performance, Permeability and Microbiota Populations of Broiler Chickens |
| title_full_unstemmed | Effects of the Antimicrobial Peptide Mastoparan X on the Performance, Permeability and Microbiota Populations of Broiler Chickens |
| title_short | Effects of the Antimicrobial Peptide Mastoparan X on the Performance, Permeability and Microbiota Populations of Broiler Chickens |
| title_sort | effects of the antimicrobial peptide mastoparan x on the performance permeability and microbiota populations of broiler chickens |
| topic | antimicrobial peptide mastoparan X broiler chickens performance intestinal health immunity |
| url | https://www.mdpi.com/2076-2615/12/24/3462 |
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