Field performance of cryopreserved seed-derived tomato plants and post-thaw survival of viral-infected meristems
The effectiveness of different cryopreservation techniques of tomato meristems isolated from viral-infected plants ‘Irishka’ cultivar was determined. The pieces of stem were protected with dimethyl sulfoxide and propylene glycol and cooled in vapour phase of liquid nitrogen (–170 °C). For the vitrif...
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2022-12-01
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| Series: | Acta Agriculturae Slovenica |
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| Online Access: | https://journals.uni-lj.si/aas/article/view/13130 |
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| author | Nadiia SHEVCHENKO Tetiana MIROSHNICHENKO Anna MOZGOVSKA Nataliia BASHTAN Galyna KOVALENKO Tetiana IVCHENKO |
| author_facet | Nadiia SHEVCHENKO Tetiana MIROSHNICHENKO Anna MOZGOVSKA Nataliia BASHTAN Galyna KOVALENKO Tetiana IVCHENKO |
| author_sort | Nadiia SHEVCHENKO |
| collection | DOAJ |
| description | The effectiveness of different cryopreservation techniques of tomato meristems isolated from viral-infected plants ‘Irishka’ cultivar was determined. The pieces of stem were protected with dimethyl sulfoxide and propylene glycol and cooled in vapour phase of liquid nitrogen (–170 °C). For the vitrification and droplet-vitrification protocols, the meristems were treated with loading solution and dehydrated with different plant vitrification solutions (PVS1 modified, PVS2, 88 % PVS3, PVSN). The samples were placed to sterilized aluminum foil pieces, in 1.2 ml cryovials or in 50 µl aluminum pans for differential scanning calorimetry and were directly immersed into liquid nitrogen. Acсording to the dehydration technique, the meristems were dehydrated with sterile airflow for 120 min. The post-thaw survival rates of meristems (from 34.2 to 78.5 %) were observerved only for 50 µl aluminum pans and airflow dehydration. We determined the productivity of plants, obtaned from cryopreserved seeds (‘Seven’, ‘Potiron Ecarlate’ and ‘Druzhba’ cultivars). We observed increasing in total and marketable yields for the plants grown from the cryopreserved seeds for all the cultivars. Total number of diseased plants decreased by 33 % for ‘Seven’, for ‘Potiron Ecarlate’ it did by 6.7 %, for that of ‘Druzhba’ the total percentage of sick and healthy plants did not differ after seeds cryopreservation.
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| first_indexed | 2024-04-09T14:41:34Z |
| format | Article |
| id | doaj.art-b3d4c7db0d1545c1b730d3f0551fe5ce |
| institution | Directory Open Access Journal |
| issn | 1854-1941 |
| language | English |
| last_indexed | 2024-04-09T14:41:34Z |
| publishDate | 2022-12-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj.art-b3d4c7db0d1545c1b730d3f0551fe5ce2023-05-03T06:36:54ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412022-12-01118410.14720/aas.2022.118.4.182319522Field performance of cryopreserved seed-derived tomato plants and post-thaw survival of viral-infected meristemsNadiia SHEVCHENKO0Tetiana MIROSHNICHENKO1Anna MOZGOVSKA2Nataliia BASHTAN3Galyna KOVALENKO4Tetiana IVCHENKO5Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of UkraineInstitute of Vegetable and Melon Growing of the National Academy of Agrarian Sciences of UkraineInstitute of Vegetable and Melon Growing of the National Academy of Agrarian Sciences of UkraineInstitute of Vegetable and Melon Growing of the National Academy of Agrarian Sciences of UkraineInstitute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of UkraineInstitute of Vegetable and Melon Growing of the National Academy of Agrarian Sciences of UkraineThe effectiveness of different cryopreservation techniques of tomato meristems isolated from viral-infected plants ‘Irishka’ cultivar was determined. The pieces of stem were protected with dimethyl sulfoxide and propylene glycol and cooled in vapour phase of liquid nitrogen (–170 °C). For the vitrification and droplet-vitrification protocols, the meristems were treated with loading solution and dehydrated with different plant vitrification solutions (PVS1 modified, PVS2, 88 % PVS3, PVSN). The samples were placed to sterilized aluminum foil pieces, in 1.2 ml cryovials or in 50 µl aluminum pans for differential scanning calorimetry and were directly immersed into liquid nitrogen. Acсording to the dehydration technique, the meristems were dehydrated with sterile airflow for 120 min. The post-thaw survival rates of meristems (from 34.2 to 78.5 %) were observerved only for 50 µl aluminum pans and airflow dehydration. We determined the productivity of plants, obtaned from cryopreserved seeds (‘Seven’, ‘Potiron Ecarlate’ and ‘Druzhba’ cultivars). We observed increasing in total and marketable yields for the plants grown from the cryopreserved seeds for all the cultivars. Total number of diseased plants decreased by 33 % for ‘Seven’, for ‘Potiron Ecarlate’ it did by 6.7 %, for that of ‘Druzhba’ the total percentage of sick and healthy plants did not differ after seeds cryopreservation. https://journals.uni-lj.si/aas/article/view/13130seed cryopreservationdehydrationmeristem cryopreservationplant vitrification solutionSolanum lycopersicum L.yield |
| spellingShingle | Nadiia SHEVCHENKO Tetiana MIROSHNICHENKO Anna MOZGOVSKA Nataliia BASHTAN Galyna KOVALENKO Tetiana IVCHENKO Field performance of cryopreserved seed-derived tomato plants and post-thaw survival of viral-infected meristems Acta Agriculturae Slovenica seed cryopreservation dehydration meristem cryopreservation plant vitrification solution Solanum lycopersicum L. yield |
| title | Field performance of cryopreserved seed-derived tomato plants and post-thaw survival of viral-infected meristems |
| title_full | Field performance of cryopreserved seed-derived tomato plants and post-thaw survival of viral-infected meristems |
| title_fullStr | Field performance of cryopreserved seed-derived tomato plants and post-thaw survival of viral-infected meristems |
| title_full_unstemmed | Field performance of cryopreserved seed-derived tomato plants and post-thaw survival of viral-infected meristems |
| title_short | Field performance of cryopreserved seed-derived tomato plants and post-thaw survival of viral-infected meristems |
| title_sort | field performance of cryopreserved seed derived tomato plants and post thaw survival of viral infected meristems |
| topic | seed cryopreservation dehydration meristem cryopreservation plant vitrification solution Solanum lycopersicum L. yield |
| url | https://journals.uni-lj.si/aas/article/view/13130 |
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