Evaluation of Bio-Friendly Formulations from Siderophore-Producing Fluorescent <i>Pseudomonas</i> as Biocontrol Agents for the Management of Soil-Borne Fungi, <i>Fusarium oxysporum</i> and <i>Rhizoctonia solani</i>
Secretion of siderophores by <i>Pseudomonas aeruginosa</i> F2 and <i>P</i>. <i>fluorescens</i> JY3 was evaluated on chrome azurol S (CAS) agar plates and their inhibitory effect was inspected against <i>Fusarium oxysporum</i> and <i>Rhizoctonia s...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/13/7/1418 |
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| author | Gaber Attia Abo-Zaid Ahmed Salah Abdullah Nadia Abdel-Mohsen Soliman Ebaa Ebrahim El-Sharouny Abdulaziz A. Al-Askar Yiming Su Ahmed Abdelkhalek Soraya Abdel-Fattah Sabry |
| author_facet | Gaber Attia Abo-Zaid Ahmed Salah Abdullah Nadia Abdel-Mohsen Soliman Ebaa Ebrahim El-Sharouny Abdulaziz A. Al-Askar Yiming Su Ahmed Abdelkhalek Soraya Abdel-Fattah Sabry |
| author_sort | Gaber Attia Abo-Zaid |
| collection | DOAJ |
| description | Secretion of siderophores by <i>Pseudomonas aeruginosa</i> F2 and <i>P</i>. <i>fluorescens</i> JY3 was evaluated on chrome azurol S (CAS) agar plates and their inhibitory effect was inspected against <i>Fusarium oxysporum</i> and <i>Rhizoctonia solani</i>. Production of siderophores as biocontrol agents from F2 and JY3 was accomplished in two optimized media. Afterward, cell-free supernatants of the bacterial cultures containing siderophores were used for the preparation of two bio-friendly formulations for the management of <i>F</i>. <i>oxysporum</i> and <i>R</i>. <i>solani</i> under greenhouse conditions. The investigated bacterial isolates, F2 and JY3, showed antagonistic activity in vitro against <i>F</i>. <i>oxysporum</i> and <i>R</i>. <i>solani</i> and produced siderophores in optimized media with high efficiency. Colonies of both bacterial isolates were grown exponentially with a constant specific growth rate of 0.07 h<sup>−1</sup> and 0.27 h<sup>−1</sup>, correspondingly. Siderophores estimated in 10 µL reached their highest value of 16.95% at 47 h and 19.5% at 48 h for isolate F2 and JY3, respectively. Formulations of siderophore-generating F2 and JY3 reduced damping-off caused by <i>F</i>. <i>oxysporum</i> by 40% and 80%, while the reduction percentage of damping-off caused by <i>R</i>. <i>solani</i> reached 87.5% and 62.5%, correspondingly. Moreover, both formulations encouraged the growing of wheat plants where the fresh and dry weight of shoots and roots were increased compared to the treatment with each fungus. In conclusion, bio-friendly formulations resulting from this investigation can play an active role in managing soil-borne diseases. |
| first_indexed | 2024-03-11T01:24:45Z |
| format | Article |
| id | doaj.art-b3d77492bed84a89902f4539e4c6a6d8 |
| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-11T01:24:45Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
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| series | Agriculture |
| spelling | doaj.art-b3d77492bed84a89902f4539e4c6a6d82023-11-18T17:53:35ZengMDPI AGAgriculture2077-04722023-07-01137141810.3390/agriculture13071418Evaluation of Bio-Friendly Formulations from Siderophore-Producing Fluorescent <i>Pseudomonas</i> as Biocontrol Agents for the Management of Soil-Borne Fungi, <i>Fusarium oxysporum</i> and <i>Rhizoctonia solani</i>Gaber Attia Abo-Zaid0Ahmed Salah Abdullah1Nadia Abdel-Mohsen Soliman2Ebaa Ebrahim El-Sharouny3Abdulaziz A. Al-Askar4Yiming Su5Ahmed Abdelkhalek6Soraya Abdel-Fattah Sabry7Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria, Alex 21934, EgyptBioprocess Development Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria, Alex 21934, EgyptBioprocess Development Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria, Alex 21934, EgyptBotany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Alex 21526, EgyptDepartment of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaUtah Water Research Laboratory, Department of Civil and Environmental Engineering, Utah State University, Logan, UT 84341, USAPlant Protection and Biomolecular Diagnosis Department, ALCRI, City of Scientific Research and Technological Applications, New Borg El Arab City, Alexandria 21934, EgyptBotany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Alex 21526, EgyptSecretion of siderophores by <i>Pseudomonas aeruginosa</i> F2 and <i>P</i>. <i>fluorescens</i> JY3 was evaluated on chrome azurol S (CAS) agar plates and their inhibitory effect was inspected against <i>Fusarium oxysporum</i> and <i>Rhizoctonia solani</i>. Production of siderophores as biocontrol agents from F2 and JY3 was accomplished in two optimized media. Afterward, cell-free supernatants of the bacterial cultures containing siderophores were used for the preparation of two bio-friendly formulations for the management of <i>F</i>. <i>oxysporum</i> and <i>R</i>. <i>solani</i> under greenhouse conditions. The investigated bacterial isolates, F2 and JY3, showed antagonistic activity in vitro against <i>F</i>. <i>oxysporum</i> and <i>R</i>. <i>solani</i> and produced siderophores in optimized media with high efficiency. Colonies of both bacterial isolates were grown exponentially with a constant specific growth rate of 0.07 h<sup>−1</sup> and 0.27 h<sup>−1</sup>, correspondingly. Siderophores estimated in 10 µL reached their highest value of 16.95% at 47 h and 19.5% at 48 h for isolate F2 and JY3, respectively. Formulations of siderophore-generating F2 and JY3 reduced damping-off caused by <i>F</i>. <i>oxysporum</i> by 40% and 80%, while the reduction percentage of damping-off caused by <i>R</i>. <i>solani</i> reached 87.5% and 62.5%, correspondingly. Moreover, both formulations encouraged the growing of wheat plants where the fresh and dry weight of shoots and roots were increased compared to the treatment with each fungus. In conclusion, bio-friendly formulations resulting from this investigation can play an active role in managing soil-borne diseases.https://www.mdpi.com/2077-0472/13/7/1418siderophoresbiocontrol<i>Pseudomonas aeruginosa</i><i>Pseudomonas fluorescens</i>bioformulation |
| spellingShingle | Gaber Attia Abo-Zaid Ahmed Salah Abdullah Nadia Abdel-Mohsen Soliman Ebaa Ebrahim El-Sharouny Abdulaziz A. Al-Askar Yiming Su Ahmed Abdelkhalek Soraya Abdel-Fattah Sabry Evaluation of Bio-Friendly Formulations from Siderophore-Producing Fluorescent <i>Pseudomonas</i> as Biocontrol Agents for the Management of Soil-Borne Fungi, <i>Fusarium oxysporum</i> and <i>Rhizoctonia solani</i> Agriculture siderophores biocontrol <i>Pseudomonas aeruginosa</i> <i>Pseudomonas fluorescens</i> bioformulation |
| title | Evaluation of Bio-Friendly Formulations from Siderophore-Producing Fluorescent <i>Pseudomonas</i> as Biocontrol Agents for the Management of Soil-Borne Fungi, <i>Fusarium oxysporum</i> and <i>Rhizoctonia solani</i> |
| title_full | Evaluation of Bio-Friendly Formulations from Siderophore-Producing Fluorescent <i>Pseudomonas</i> as Biocontrol Agents for the Management of Soil-Borne Fungi, <i>Fusarium oxysporum</i> and <i>Rhizoctonia solani</i> |
| title_fullStr | Evaluation of Bio-Friendly Formulations from Siderophore-Producing Fluorescent <i>Pseudomonas</i> as Biocontrol Agents for the Management of Soil-Borne Fungi, <i>Fusarium oxysporum</i> and <i>Rhizoctonia solani</i> |
| title_full_unstemmed | Evaluation of Bio-Friendly Formulations from Siderophore-Producing Fluorescent <i>Pseudomonas</i> as Biocontrol Agents for the Management of Soil-Borne Fungi, <i>Fusarium oxysporum</i> and <i>Rhizoctonia solani</i> |
| title_short | Evaluation of Bio-Friendly Formulations from Siderophore-Producing Fluorescent <i>Pseudomonas</i> as Biocontrol Agents for the Management of Soil-Borne Fungi, <i>Fusarium oxysporum</i> and <i>Rhizoctonia solani</i> |
| title_sort | evaluation of bio friendly formulations from siderophore producing fluorescent i pseudomonas i as biocontrol agents for the management of soil borne fungi i fusarium oxysporum i and i rhizoctonia solani i |
| topic | siderophores biocontrol <i>Pseudomonas aeruginosa</i> <i>Pseudomonas fluorescens</i> bioformulation |
| url | https://www.mdpi.com/2077-0472/13/7/1418 |
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