A Green Nanostructured Pesticide to Control Tomato Bacterial Speck Disease
Bacterial speck disease, caused by <i>Pseudomonas syringae</i> pv. <i>tomato</i> (Pst), is one of the most pervasive biological adversities in tomato cultivation, in both industrial and in table varieties. In this work synthesis, biochemical and antibacterial properties of a...
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MDPI AG
2021-07-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/7/1852 |
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| author | Daniele Schiavi Rosa Balbi Stefano Giovagnoli Emidio Camaioni Ermelinda Botticella Francesco Sestili Giorgio Mariano Balestra |
| author_facet | Daniele Schiavi Rosa Balbi Stefano Giovagnoli Emidio Camaioni Ermelinda Botticella Francesco Sestili Giorgio Mariano Balestra |
| author_sort | Daniele Schiavi |
| collection | DOAJ |
| description | Bacterial speck disease, caused by <i>Pseudomonas syringae</i> pv. <i>tomato</i> (Pst), is one of the most pervasive biological adversities in tomato cultivation, in both industrial and in table varieties. In this work synthesis, biochemical and antibacterial properties of a novel organic nanostructured pesticide composed of chitosan hydrochloride (CH) as active ingredient, cellulose nanocrystals (CNC) as nanocarriers and starch as excipient were evaluated. In order to study the possibility of delivering CH, the effects of two different types of starches, extracted from a high amylose bread wheat (high amylose starch—HA Starch) and from a control genotype (standard starch—St Starch), were investigated. Nanostructured microparticles (NMP) were obtained through the spray-drying technique, revealing a CH loading capacity proximal to 50%, with a CH release of 30% for CH-CNC-St Starch NMP and 50% for CH-CNC-HA Starch NMP after 24 h. Both NMP were able to inhibit bacterial growth in vitro when used at 1% w/v. Moreover, no negative effects on vegetative growth were recorded when NMP were foliar applied on tomato plants. Proposed nanostructured pesticides showed the capability of diminishing Pst epiphytical survival during time, decreasing disease incidence and severity (from 45% to 49%), with results comparable to one of the most used cupric salt (hydroxide), pointing out the potential use of CH-CNC-Starch NMP as a sustainable and innovative ally in Pst control strategies. |
| first_indexed | 2024-03-10T09:29:46Z |
| format | Article |
| id | doaj.art-b3dc8d689e4c48499deb49c31bdab6e7 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T09:29:46Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-b3dc8d689e4c48499deb49c31bdab6e72023-11-22T04:34:56ZengMDPI AGNanomaterials2079-49912021-07-01117185210.3390/nano11071852A Green Nanostructured Pesticide to Control Tomato Bacterial Speck DiseaseDaniele Schiavi0Rosa Balbi1Stefano Giovagnoli2Emidio Camaioni3Ermelinda Botticella4Francesco Sestili5Giorgio Mariano Balestra6Department of Agricultural and Forestry Sciences (DAFNE), University of Tuscia, Via San Camillo de Lellis snc, 01100 Viterbo, ItalyDepartment of Pharmaceutical Sciences (DSF), University of Perugia, Via del Liceo 1, 06123 Perugia, ItalyDepartment of Pharmaceutical Sciences (DSF), University of Perugia, Via del Liceo 1, 06123 Perugia, ItalyDepartment of Pharmaceutical Sciences (DSF), University of Perugia, Via del Liceo 1, 06123 Perugia, ItalyDepartment of Agricultural and Forestry Sciences (DAFNE), University of Tuscia, Via San Camillo de Lellis snc, 01100 Viterbo, ItalyDepartment of Agricultural and Forestry Sciences (DAFNE), University of Tuscia, Via San Camillo de Lellis snc, 01100 Viterbo, ItalyDepartment of Agricultural and Forestry Sciences (DAFNE), University of Tuscia, Via San Camillo de Lellis snc, 01100 Viterbo, ItalyBacterial speck disease, caused by <i>Pseudomonas syringae</i> pv. <i>tomato</i> (Pst), is one of the most pervasive biological adversities in tomato cultivation, in both industrial and in table varieties. In this work synthesis, biochemical and antibacterial properties of a novel organic nanostructured pesticide composed of chitosan hydrochloride (CH) as active ingredient, cellulose nanocrystals (CNC) as nanocarriers and starch as excipient were evaluated. In order to study the possibility of delivering CH, the effects of two different types of starches, extracted from a high amylose bread wheat (high amylose starch—HA Starch) and from a control genotype (standard starch—St Starch), were investigated. Nanostructured microparticles (NMP) were obtained through the spray-drying technique, revealing a CH loading capacity proximal to 50%, with a CH release of 30% for CH-CNC-St Starch NMP and 50% for CH-CNC-HA Starch NMP after 24 h. Both NMP were able to inhibit bacterial growth in vitro when used at 1% w/v. Moreover, no negative effects on vegetative growth were recorded when NMP were foliar applied on tomato plants. Proposed nanostructured pesticides showed the capability of diminishing Pst epiphytical survival during time, decreasing disease incidence and severity (from 45% to 49%), with results comparable to one of the most used cupric salt (hydroxide), pointing out the potential use of CH-CNC-Starch NMP as a sustainable and innovative ally in Pst control strategies.https://www.mdpi.com/2079-4991/11/7/1852cellulose nanocrystalschitosan hydrochloridestarch<i>pseudomonas syringae</i> pv. <i>tomato</i>tomatonanotechnology |
| spellingShingle | Daniele Schiavi Rosa Balbi Stefano Giovagnoli Emidio Camaioni Ermelinda Botticella Francesco Sestili Giorgio Mariano Balestra A Green Nanostructured Pesticide to Control Tomato Bacterial Speck Disease Nanomaterials cellulose nanocrystals chitosan hydrochloride starch <i>pseudomonas syringae</i> pv. <i>tomato</i> tomato nanotechnology |
| title | A Green Nanostructured Pesticide to Control Tomato Bacterial Speck Disease |
| title_full | A Green Nanostructured Pesticide to Control Tomato Bacterial Speck Disease |
| title_fullStr | A Green Nanostructured Pesticide to Control Tomato Bacterial Speck Disease |
| title_full_unstemmed | A Green Nanostructured Pesticide to Control Tomato Bacterial Speck Disease |
| title_short | A Green Nanostructured Pesticide to Control Tomato Bacterial Speck Disease |
| title_sort | green nanostructured pesticide to control tomato bacterial speck disease |
| topic | cellulose nanocrystals chitosan hydrochloride starch <i>pseudomonas syringae</i> pv. <i>tomato</i> tomato nanotechnology |
| url | https://www.mdpi.com/2079-4991/11/7/1852 |
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