Trichostrongyloid nematodes in ruminants of northern Iran: prevalence and molecular analysis
Abstract Background This study was carried out to investigate the prevalence and analyze the molecular characteristics based on the internal transcribed spacer (ITS) 2 region of the ribosomal RNA (RNA) gene of trichostrongylid nematodes in different ruminants from Guilan province, northern of Iran....
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| Format: | Article |
| Language: | English |
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BMC
2021-12-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-021-03086-3 |
| _version_ | 1818574687731449856 |
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| author | Hedayat Hosseinnezhad Meysam Sharifdini Keyhan Ashrafi Zahra Atrkar Roushan Hamed Mirjalali Behnaz Rahmati |
| author_facet | Hedayat Hosseinnezhad Meysam Sharifdini Keyhan Ashrafi Zahra Atrkar Roushan Hamed Mirjalali Behnaz Rahmati |
| author_sort | Hedayat Hosseinnezhad |
| collection | DOAJ |
| description | Abstract Background This study was carried out to investigate the prevalence and analyze the molecular characteristics based on the internal transcribed spacer (ITS) 2 region of the ribosomal RNA (RNA) gene of trichostrongylid nematodes in different ruminants from Guilan province, northern of Iran. Methods The gastrointestinal tracts of 144 ruminants including 72 cattle, 59 sheep, and 13 goats were collected from an abattoir in Guilan province during July to September 2018. After isolation the helminths, male specimens were identified based on morphological parameters. PCR and partial sequencing of the ITS2 fragment were conducted. After phylogenetic analysis, the intraspecific and interspecific differences were calculated. Results The prevalence of total infections with the nematodes was 38.9, 74.6 and 84.6% among cattle, sheep and goats, respectively. Eleven species of trichostrongylid nematodes including Haemonchus contortus, Marshallagia marshalli, Trichostrongylus axei, T. colubriformis, T. vitrinus, Ostertagia trifurcata, Teladorsagia circumcincta, Marshallagia occidentalis, O. lyrata, O. ostertagi, and Cooperia punctate were recovered from the ruminants. The most prevalent trichostrongyloid nematodes in cattle, sheep and goats were O. ostertagi (26.4%), M. marshalli (64.4%) and T. circumcincta (69.2%), respectively. Phylogenetic tree was discriminative for Trichostrongylidae family, while phylogenetic analysis of the ITS2 gene represented low variations and no species identification of Haemonchidae and Cooperiidae families. Conclusions This study suggests the high prevalence and species diversity of trichostrongyloid nematodes in different ruminants, indicating the importance of implement antiparasitic strategies in north regions of Iran. As well, this study showed that the ITS2 fragment is not a discriminative marker for Haemonchidae and Cooperiidae families, and investigation of other genetic markers such as mitochondrial genes would be more valuable for better understanding of their phylogenetic relationships. |
| first_indexed | 2024-12-15T00:29:51Z |
| format | Article |
| id | doaj.art-b3de44c7b14146f388f5cea3f53ad633 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-15T00:29:51Z |
| publishDate | 2021-12-01 |
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| series | BMC Veterinary Research |
| spelling | doaj.art-b3de44c7b14146f388f5cea3f53ad6332022-12-21T22:42:03ZengBMCBMC Veterinary Research1746-61482021-12-0117111210.1186/s12917-021-03086-3Trichostrongyloid nematodes in ruminants of northern Iran: prevalence and molecular analysisHedayat Hosseinnezhad0Meysam Sharifdini1Keyhan Ashrafi2Zahra Atrkar Roushan3Hamed Mirjalali4Behnaz Rahmati5Department of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical SciencesDepartment of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical SciencesDepartment of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical SciencesDepartment of Biostatistics, School of Medicine, Guilan University of Medical SciencesFoodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical SciencesDepartment of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical SciencesAbstract Background This study was carried out to investigate the prevalence and analyze the molecular characteristics based on the internal transcribed spacer (ITS) 2 region of the ribosomal RNA (RNA) gene of trichostrongylid nematodes in different ruminants from Guilan province, northern of Iran. Methods The gastrointestinal tracts of 144 ruminants including 72 cattle, 59 sheep, and 13 goats were collected from an abattoir in Guilan province during July to September 2018. After isolation the helminths, male specimens were identified based on morphological parameters. PCR and partial sequencing of the ITS2 fragment were conducted. After phylogenetic analysis, the intraspecific and interspecific differences were calculated. Results The prevalence of total infections with the nematodes was 38.9, 74.6 and 84.6% among cattle, sheep and goats, respectively. Eleven species of trichostrongylid nematodes including Haemonchus contortus, Marshallagia marshalli, Trichostrongylus axei, T. colubriformis, T. vitrinus, Ostertagia trifurcata, Teladorsagia circumcincta, Marshallagia occidentalis, O. lyrata, O. ostertagi, and Cooperia punctate were recovered from the ruminants. The most prevalent trichostrongyloid nematodes in cattle, sheep and goats were O. ostertagi (26.4%), M. marshalli (64.4%) and T. circumcincta (69.2%), respectively. Phylogenetic tree was discriminative for Trichostrongylidae family, while phylogenetic analysis of the ITS2 gene represented low variations and no species identification of Haemonchidae and Cooperiidae families. Conclusions This study suggests the high prevalence and species diversity of trichostrongyloid nematodes in different ruminants, indicating the importance of implement antiparasitic strategies in north regions of Iran. As well, this study showed that the ITS2 fragment is not a discriminative marker for Haemonchidae and Cooperiidae families, and investigation of other genetic markers such as mitochondrial genes would be more valuable for better understanding of their phylogenetic relationships.https://doi.org/10.1186/s12917-021-03086-3Trichostrongyloid nematodesRuminantsITS2Molecular analysisIran |
| spellingShingle | Hedayat Hosseinnezhad Meysam Sharifdini Keyhan Ashrafi Zahra Atrkar Roushan Hamed Mirjalali Behnaz Rahmati Trichostrongyloid nematodes in ruminants of northern Iran: prevalence and molecular analysis BMC Veterinary Research Trichostrongyloid nematodes Ruminants ITS2 Molecular analysis Iran |
| title | Trichostrongyloid nematodes in ruminants of northern Iran: prevalence and molecular analysis |
| title_full | Trichostrongyloid nematodes in ruminants of northern Iran: prevalence and molecular analysis |
| title_fullStr | Trichostrongyloid nematodes in ruminants of northern Iran: prevalence and molecular analysis |
| title_full_unstemmed | Trichostrongyloid nematodes in ruminants of northern Iran: prevalence and molecular analysis |
| title_short | Trichostrongyloid nematodes in ruminants of northern Iran: prevalence and molecular analysis |
| title_sort | trichostrongyloid nematodes in ruminants of northern iran prevalence and molecular analysis |
| topic | Trichostrongyloid nematodes Ruminants ITS2 Molecular analysis Iran |
| url | https://doi.org/10.1186/s12917-021-03086-3 |
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