Nucleotide Base Variation of Blast Disease Resistance Gene Pi33 in Rice Selected Broad Genetic Background
Rice is one of the most important crops for human beings, thus increasing productivity are continually persecuted. Blast disease can reduce the rate of productivity of rice cultivation. Therefore, the program of blast disease-resistant varieties needs to do effectively. One of broad-spectrum blast d...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2011-09-01
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| Series: | Hayati Journal of Biosciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S197830191630198X |
| _version_ | 1818533477364006912 |
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| author | DWINITA WIKAN UTAMI KALIA BARNITA SITI YURIAH IDA HANARIDA |
| author_facet | DWINITA WIKAN UTAMI KALIA BARNITA SITI YURIAH IDA HANARIDA |
| author_sort | DWINITA WIKAN UTAMI |
| collection | DOAJ |
| description | Rice is one of the most important crops for human beings, thus increasing productivity are continually persecuted. Blast disease can reduce the rate of productivity of rice cultivation. Therefore, the program of blast disease-resistant varieties needs to do effectively. One of broad-spectrum blast disease-resistant gene is Pi33. This study was aimed to identify the variation in the sequence of nucleotide bases of Pi33 gene in five interspesific lines which derived from Bio46 (IR64/Oryza rufipogon) and CT13432 crossing. DNA of five rice lines were amplified using the spesific primer for Pi33, G1010. Amplification results purified through Exonuclease 1 and Shrimp Alkaline Phosphatase protocols. Labelling using fluorescent dyes done before sequencing nucleotide base using CEQ8000 instrument. The results showed that lines number 28 showed introgesion of the three control parent genome (subspecies of Indica, subspecies of Japonica, and O. rufipogon) while the Lines number 79, 136, and 143 were identical to Indica genome. Strain number 195 was identical to Japonica genome. These broad genetic background lines promise as durable performance to attack the expansion of the dynamic nature of the pathogen to blast. The result of ortholog sequence analysis found conserved nucleotide base sequence (CAGCAGCC) which involved in heterotrimeric G-protein group. This protein has role as plant receptor for recognizing pathogen elicitor in interaction of rice and blast pathogen. |
| first_indexed | 2024-12-11T17:59:13Z |
| format | Article |
| id | doaj.art-b3deaa21a2a44a77ba1f4297e964b791 |
| institution | Directory Open Access Journal |
| issn | 1978-3019 |
| language | English |
| last_indexed | 2024-12-11T17:59:13Z |
| publishDate | 2011-09-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-b3deaa21a2a44a77ba1f4297e964b7912022-12-22T00:55:58ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192011-09-0118312312810.4308/hjb.18.3.123Nucleotide Base Variation of Blast Disease Resistance Gene Pi33 in Rice Selected Broad Genetic BackgroundDWINITA WIKAN UTAMI0KALIA BARNITA1SITI YURIAH2IDA HANARIDA3Indonesian Centre of Biotechnology and Genetic Resources of Agriculture, Research and Development, Jalan Tentara Pelajar No. 3A, Bogor 16111, IndonesiaDepartment of Biochemistry, Faculty of Mathematics and Nature Science, Bogor Agriculture University, Darmaga Campus, Bogor 16680, IndonesiaIndonesian Centre of Biotechnology and Genetic Resources of Agriculture, Research and Development, Jalan Tentara Pelajar No. 3A, Bogor 16111, IndonesiaIndonesian Centre of Biotechnology and Genetic Resources of Agriculture, Research and Development, Jalan Tentara Pelajar No. 3A, Bogor 16111, IndonesiaRice is one of the most important crops for human beings, thus increasing productivity are continually persecuted. Blast disease can reduce the rate of productivity of rice cultivation. Therefore, the program of blast disease-resistant varieties needs to do effectively. One of broad-spectrum blast disease-resistant gene is Pi33. This study was aimed to identify the variation in the sequence of nucleotide bases of Pi33 gene in five interspesific lines which derived from Bio46 (IR64/Oryza rufipogon) and CT13432 crossing. DNA of five rice lines were amplified using the spesific primer for Pi33, G1010. Amplification results purified through Exonuclease 1 and Shrimp Alkaline Phosphatase protocols. Labelling using fluorescent dyes done before sequencing nucleotide base using CEQ8000 instrument. The results showed that lines number 28 showed introgesion of the three control parent genome (subspecies of Indica, subspecies of Japonica, and O. rufipogon) while the Lines number 79, 136, and 143 were identical to Indica genome. Strain number 195 was identical to Japonica genome. These broad genetic background lines promise as durable performance to attack the expansion of the dynamic nature of the pathogen to blast. The result of ortholog sequence analysis found conserved nucleotide base sequence (CAGCAGCC) which involved in heterotrimeric G-protein group. This protein has role as plant receptor for recognizing pathogen elicitor in interaction of rice and blast pathogen.http://www.sciencedirect.com/science/article/pii/S197830191630198Xnucleotide baseblast diseasePi33 generice |
| spellingShingle | DWINITA WIKAN UTAMI KALIA BARNITA SITI YURIAH IDA HANARIDA Nucleotide Base Variation of Blast Disease Resistance Gene Pi33 in Rice Selected Broad Genetic Background Hayati Journal of Biosciences nucleotide base blast disease Pi33 gene rice |
| title | Nucleotide Base Variation of Blast Disease Resistance Gene Pi33 in Rice Selected Broad Genetic Background |
| title_full | Nucleotide Base Variation of Blast Disease Resistance Gene Pi33 in Rice Selected Broad Genetic Background |
| title_fullStr | Nucleotide Base Variation of Blast Disease Resistance Gene Pi33 in Rice Selected Broad Genetic Background |
| title_full_unstemmed | Nucleotide Base Variation of Blast Disease Resistance Gene Pi33 in Rice Selected Broad Genetic Background |
| title_short | Nucleotide Base Variation of Blast Disease Resistance Gene Pi33 in Rice Selected Broad Genetic Background |
| title_sort | nucleotide base variation of blast disease resistance gene pi33 in rice selected broad genetic background |
| topic | nucleotide base blast disease Pi33 gene rice |
| url | http://www.sciencedirect.com/science/article/pii/S197830191630198X |
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