Is Acteoside Effects on Colon Cancer Stem Cells Via Inflamation or Apoptosis?
AIM: Colon cancer is one of the main health problems worldwide. Cancer stem cells (CSCs) are referred to as tumor-initiating cells involved in tumor heterogeneity and dormancy. CSCs can cause drug resistance, metastasis, and recurrence of primary and metastatic cancers. The interactions and survival...
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| Format: | Article |
| Language: | English |
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Selcuk University Press
2022-08-01
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| Series: | Genel Tıp Dergisi |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/2174923 |
| _version_ | 1826906099620511744 |
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| author | Hafize Seda Vatansever Feyzan Ozdal Kurt Canan Türkoğlu Fatma Fırat |
| author_facet | Hafize Seda Vatansever Feyzan Ozdal Kurt Canan Türkoğlu Fatma Fırat |
| author_sort | Hafize Seda Vatansever |
| collection | DOAJ |
| description | AIM: Colon cancer is one of the main health problems worldwide. Cancer stem cells (CSCs) are referred to as tumor-initiating cells involved in tumor heterogeneity and dormancy. CSCs can cause drug resistance, metastasis, and recurrence of primary and metastatic cancers. The interactions and survival trends of colon cancer stem cells with other cells may be an alternative route for effective treatment. In our study, we aimed to evaluate the effects of asteoside on stem cell properties, apoptotic and inflammatory processes in primary (HCT-116) and metastatic (Colo-741) colon CSCs.
METHERIALS AND METHODS: CSCs were obtained from both types of colon cancer cell lines with the MINIMACS system using the anti-CD133 reagent. Metastatic Colo-741 and non-metastatic HCT-116 CD133+ and CD133- cells were cultured with or without Acteoside for 48 hours. Expressions of Caspase-3, Bcl, Bax, and Fas-L for apoptosis, and IL-1β, TNF-α, IL-6, IL8 and IL-10 for inflammation were analyzed by indirect immunocytochemistry technique by performing H-Score. Changes in cell morphology were examined under an inverted microscope.
RESULTS AND CONCLUSION: It was observed that Oct-4 expression decreased after acteocyte administration in both metastatic and non-metastatic colon cancer cells. In addition, Colo-741 increased the intensity of the Bax/Bcl ratio in both CD133+ and CD133- cells and increased caspase 3 expression. While acteoside did not immunohistochemically affect inflammation in metastatic COLO-741 cells, it contributed to the apoptotic process by changing the Bax/Bcl ratio. When the morphology of the cells was evaluated, it was observed that the number of apoptotic cells increased in COLO-741 CD133+ and CD133- cells. |
| first_indexed | 2024-03-12T01:07:11Z |
| format | Article |
| id | doaj.art-b3dfb6c865c04bb1b0fbafc0ab30a9b4 |
| institution | Directory Open Access Journal |
| issn | 2602-3741 |
| language | English |
| last_indexed | 2025-02-17T08:41:18Z |
| publishDate | 2022-08-01 |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Genel Tıp Dergisi |
| spelling | doaj.art-b3dfb6c865c04bb1b0fbafc0ab30a9b42025-01-02T21:15:06ZengSelcuk University PressGenel Tıp Dergisi2602-37412022-08-0132437237910.54005/geneltip.1053439154Is Acteoside Effects on Colon Cancer Stem Cells Via Inflamation or Apoptosis?Hafize Seda Vatansever0Feyzan Ozdal Kurt1Canan Türkoğlu2Fatma Fırat3MANİSA CELÂL BAYAR ÜNİVERSİTESİ, SAĞLIK BİLİMLERİ ENSTİTÜSÜ, HİSTOLOJİ EMBRİYOLOJİ (DR)MANİSA CELÂL BAYAR ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ, BİYOLOJİ BÖLÜMÜ, BİYOLOJİ PR.CELÂL BAYAR ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ, BİYOLOJİ BÖLÜMÜ, BİYOLOJİ PR.Afyonkarahisar Sağlık Bilimleri ÜniversitesiAIM: Colon cancer is one of the main health problems worldwide. Cancer stem cells (CSCs) are referred to as tumor-initiating cells involved in tumor heterogeneity and dormancy. CSCs can cause drug resistance, metastasis, and recurrence of primary and metastatic cancers. The interactions and survival trends of colon cancer stem cells with other cells may be an alternative route for effective treatment. In our study, we aimed to evaluate the effects of asteoside on stem cell properties, apoptotic and inflammatory processes in primary (HCT-116) and metastatic (Colo-741) colon CSCs. METHERIALS AND METHODS: CSCs were obtained from both types of colon cancer cell lines with the MINIMACS system using the anti-CD133 reagent. Metastatic Colo-741 and non-metastatic HCT-116 CD133+ and CD133- cells were cultured with or without Acteoside for 48 hours. Expressions of Caspase-3, Bcl, Bax, and Fas-L for apoptosis, and IL-1β, TNF-α, IL-6, IL8 and IL-10 for inflammation were analyzed by indirect immunocytochemistry technique by performing H-Score. Changes in cell morphology were examined under an inverted microscope. RESULTS AND CONCLUSION: It was observed that Oct-4 expression decreased after acteocyte administration in both metastatic and non-metastatic colon cancer cells. In addition, Colo-741 increased the intensity of the Bax/Bcl ratio in both CD133+ and CD133- cells and increased caspase 3 expression. While acteoside did not immunohistochemically affect inflammation in metastatic COLO-741 cells, it contributed to the apoptotic process by changing the Bax/Bcl ratio. When the morphology of the cells was evaluated, it was observed that the number of apoptotic cells increased in COLO-741 CD133+ and CD133- cells.https://dergipark.org.tr/tr/download/article-file/2174923kolon kanseri kök hücrelericolo-741hct-116apoptozinflamasyonacteosit.colon cancer stem cellscolo-741hct-116apoptosisinflammationacteoside. |
| spellingShingle | Hafize Seda Vatansever Feyzan Ozdal Kurt Canan Türkoğlu Fatma Fırat Is Acteoside Effects on Colon Cancer Stem Cells Via Inflamation or Apoptosis? Genel Tıp Dergisi kolon kanseri kök hücreleri colo-741 hct-116 apoptoz inflamasyon acteosit. colon cancer stem cells colo-741 hct-116 apoptosis inflammation acteoside. |
| title | Is Acteoside Effects on Colon Cancer Stem Cells Via Inflamation or Apoptosis? |
| title_full | Is Acteoside Effects on Colon Cancer Stem Cells Via Inflamation or Apoptosis? |
| title_fullStr | Is Acteoside Effects on Colon Cancer Stem Cells Via Inflamation or Apoptosis? |
| title_full_unstemmed | Is Acteoside Effects on Colon Cancer Stem Cells Via Inflamation or Apoptosis? |
| title_short | Is Acteoside Effects on Colon Cancer Stem Cells Via Inflamation or Apoptosis? |
| title_sort | is acteoside effects on colon cancer stem cells via inflamation or apoptosis |
| topic | kolon kanseri kök hücreleri colo-741 hct-116 apoptoz inflamasyon acteosit. colon cancer stem cells colo-741 hct-116 apoptosis inflammation acteoside. |
| url | https://dergipark.org.tr/tr/download/article-file/2174923 |
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