AT-hook DNA-binding motif-containing protein one knockdown downregulates EWS-FLI1 transcriptional activity in Ewing’s sarcoma cells
Ewing’s sarcoma is the second most common bone malignancy in children or young adults and is caused by an oncogenic transcription factor by a chromosomal translocation between the EWSR1 gene and the ETS transcription factor family. However, the transcriptional mechanism of EWS-ETS fusion proteins is...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9531837/?tool=EBI |
| _version_ | 1797997144950439936 |
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| author | Takao Kitagawa Daiki Kobayashi Byron Baron Hajime Okita Tatsuo Miyamoto Rie Takai Durga Paudel Tohru Ohta Yoichi Asaoka Masayuki Tokunaga Koji Nakagawa Makoto Furutani-Seiki Norie Araki Yasuhiro Kuramitsu Masanobu Kobayashi |
| author_facet | Takao Kitagawa Daiki Kobayashi Byron Baron Hajime Okita Tatsuo Miyamoto Rie Takai Durga Paudel Tohru Ohta Yoichi Asaoka Masayuki Tokunaga Koji Nakagawa Makoto Furutani-Seiki Norie Araki Yasuhiro Kuramitsu Masanobu Kobayashi |
| author_sort | Takao Kitagawa |
| collection | DOAJ |
| description | Ewing’s sarcoma is the second most common bone malignancy in children or young adults and is caused by an oncogenic transcription factor by a chromosomal translocation between the EWSR1 gene and the ETS transcription factor family. However, the transcriptional mechanism of EWS-ETS fusion proteins is still unclear. To identify the transcriptional complexes of EWS-ETS fusion transcription factors, we applied a proximal labeling system called BioID in Ewing’s sarcoma cells. We identified AHDC1 as a proximal protein of EWS-ETS fusion proteins. AHDC1 knockdown showed a reduced cell growth and transcriptional activity of EWS-FLI1. AHDC1 knockdown also reduced BRD4 and BRG1 protein levels, both known as interacting proteins of EWS-FLI1. Our results suggest that AHDC1 supports cell growth through EWS-FLI1. |
| first_indexed | 2024-04-11T10:28:15Z |
| format | Article |
| id | doaj.art-b3e216d645cb41128dbf5f0f447ef6f2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T10:28:15Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b3e216d645cb41128dbf5f0f447ef6f22022-12-22T04:29:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-011710AT-hook DNA-binding motif-containing protein one knockdown downregulates EWS-FLI1 transcriptional activity in Ewing’s sarcoma cellsTakao KitagawaDaiki KobayashiByron BaronHajime OkitaTatsuo MiyamotoRie TakaiDurga PaudelTohru OhtaYoichi AsaokaMasayuki TokunagaKoji NakagawaMakoto Furutani-SeikiNorie ArakiYasuhiro KuramitsuMasanobu KobayashiEwing’s sarcoma is the second most common bone malignancy in children or young adults and is caused by an oncogenic transcription factor by a chromosomal translocation between the EWSR1 gene and the ETS transcription factor family. However, the transcriptional mechanism of EWS-ETS fusion proteins is still unclear. To identify the transcriptional complexes of EWS-ETS fusion transcription factors, we applied a proximal labeling system called BioID in Ewing’s sarcoma cells. We identified AHDC1 as a proximal protein of EWS-ETS fusion proteins. AHDC1 knockdown showed a reduced cell growth and transcriptional activity of EWS-FLI1. AHDC1 knockdown also reduced BRD4 and BRG1 protein levels, both known as interacting proteins of EWS-FLI1. Our results suggest that AHDC1 supports cell growth through EWS-FLI1.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9531837/?tool=EBI |
| spellingShingle | Takao Kitagawa Daiki Kobayashi Byron Baron Hajime Okita Tatsuo Miyamoto Rie Takai Durga Paudel Tohru Ohta Yoichi Asaoka Masayuki Tokunaga Koji Nakagawa Makoto Furutani-Seiki Norie Araki Yasuhiro Kuramitsu Masanobu Kobayashi AT-hook DNA-binding motif-containing protein one knockdown downregulates EWS-FLI1 transcriptional activity in Ewing’s sarcoma cells PLoS ONE |
| title | AT-hook DNA-binding motif-containing protein one knockdown downregulates EWS-FLI1 transcriptional activity in Ewing’s sarcoma cells |
| title_full | AT-hook DNA-binding motif-containing protein one knockdown downregulates EWS-FLI1 transcriptional activity in Ewing’s sarcoma cells |
| title_fullStr | AT-hook DNA-binding motif-containing protein one knockdown downregulates EWS-FLI1 transcriptional activity in Ewing’s sarcoma cells |
| title_full_unstemmed | AT-hook DNA-binding motif-containing protein one knockdown downregulates EWS-FLI1 transcriptional activity in Ewing’s sarcoma cells |
| title_short | AT-hook DNA-binding motif-containing protein one knockdown downregulates EWS-FLI1 transcriptional activity in Ewing’s sarcoma cells |
| title_sort | at hook dna binding motif containing protein one knockdown downregulates ews fli1 transcriptional activity in ewing s sarcoma cells |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9531837/?tool=EBI |
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