DNA damage repair proteins across the Tree of Life
Summary: Genome maintenance is orchestrated by a highly regulated DNA damage response with specific DNA repair pathways. Here, we investigate the phylogenetic diversity in the recognition and repair of three well-established DNA lesions, primarily repaired by base excision repair (BER) and ribonucle...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223008556 |
| _version_ | 1797822073539657728 |
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| author | Emily Nischwitz Vivien A.C. Schoonenberg Albert Fradera-Sola Mario Dejung Olga Vydzhak Michal Levin Brian Luke Falk Butter Marion Scheibe |
| author_facet | Emily Nischwitz Vivien A.C. Schoonenberg Albert Fradera-Sola Mario Dejung Olga Vydzhak Michal Levin Brian Luke Falk Butter Marion Scheibe |
| author_sort | Emily Nischwitz |
| collection | DOAJ |
| description | Summary: Genome maintenance is orchestrated by a highly regulated DNA damage response with specific DNA repair pathways. Here, we investigate the phylogenetic diversity in the recognition and repair of three well-established DNA lesions, primarily repaired by base excision repair (BER) and ribonucleotide excision repair (RER): (1) 8-oxoguanine, (2) abasic site, and (3) incorporated ribonucleotide in DNA in 11 species: Escherichia coli, Bacillus subtilis, Halobacterium salinarum, Trypanosoma brucei, Tetrahymena thermophila, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Caenorhabditis elegans, Homo sapiens, Arabidopsis thaliana, and Zea mays. Using quantitative mass spectrometry, we identified 337 binding proteins across these species. Of these proteins, 99 were previously characterized to be involved in DNA repair. Through orthology, network, and domain analysis, we linked 44 previously unconnected proteins to DNA repair. Our study presents a resource for future study of the crosstalk and evolutionary conservation of DNA damage repair across all domains of life. |
| first_indexed | 2024-03-13T10:03:29Z |
| format | Article |
| id | doaj.art-b3e45d4033424ad4a42ae087cd3a30f6 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-13T10:03:29Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-b3e45d4033424ad4a42ae087cd3a30f62023-05-23T04:21:58ZengElsevieriScience2589-00422023-06-01266106778DNA damage repair proteins across the Tree of LifeEmily Nischwitz0Vivien A.C. Schoonenberg1Albert Fradera-Sola2Mario Dejung3Olga Vydzhak4Michal Levin5Brian Luke6Falk Butter7Marion Scheibe8Institute of Molecular Biology (IMB), 55128 Mainz, GermanyInstitute of Molecular Biology (IMB), 55128 Mainz, GermanyInstitute of Molecular Biology (IMB), 55128 Mainz, GermanyInstitute of Molecular Biology (IMB), 55128 Mainz, GermanyInstitute of Developmental Biology and Neurobiology (IDN), Johannes-Gutenberg-University, 55128 Mainz, GermanyInstitute of Molecular Biology (IMB), 55128 Mainz, GermanyInstitute of Molecular Biology (IMB), 55128 Mainz, Germany; Institute of Developmental Biology and Neurobiology (IDN), Johannes-Gutenberg-University, 55128 Mainz, GermanyInstitute of Molecular Biology (IMB), 55128 Mainz, Germany; Corresponding authorInstitute of Molecular Biology (IMB), 55128 Mainz, Germany; Corresponding authorSummary: Genome maintenance is orchestrated by a highly regulated DNA damage response with specific DNA repair pathways. Here, we investigate the phylogenetic diversity in the recognition and repair of three well-established DNA lesions, primarily repaired by base excision repair (BER) and ribonucleotide excision repair (RER): (1) 8-oxoguanine, (2) abasic site, and (3) incorporated ribonucleotide in DNA in 11 species: Escherichia coli, Bacillus subtilis, Halobacterium salinarum, Trypanosoma brucei, Tetrahymena thermophila, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Caenorhabditis elegans, Homo sapiens, Arabidopsis thaliana, and Zea mays. Using quantitative mass spectrometry, we identified 337 binding proteins across these species. Of these proteins, 99 were previously characterized to be involved in DNA repair. Through orthology, network, and domain analysis, we linked 44 previously unconnected proteins to DNA repair. Our study presents a resource for future study of the crosstalk and evolutionary conservation of DNA damage repair across all domains of life.http://www.sciencedirect.com/science/article/pii/S2589004223008556PhylogeneticsMolecular biologyEvolutionary biology |
| spellingShingle | Emily Nischwitz Vivien A.C. Schoonenberg Albert Fradera-Sola Mario Dejung Olga Vydzhak Michal Levin Brian Luke Falk Butter Marion Scheibe DNA damage repair proteins across the Tree of Life iScience Phylogenetics Molecular biology Evolutionary biology |
| title | DNA damage repair proteins across the Tree of Life |
| title_full | DNA damage repair proteins across the Tree of Life |
| title_fullStr | DNA damage repair proteins across the Tree of Life |
| title_full_unstemmed | DNA damage repair proteins across the Tree of Life |
| title_short | DNA damage repair proteins across the Tree of Life |
| title_sort | dna damage repair proteins across the tree of life |
| topic | Phylogenetics Molecular biology Evolutionary biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223008556 |
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