Reassessment of the Phylogenetics of Two Pygmy Grasshopper Generic Groups <i>Tetrix</i> and <i>Systolederus</i> through Mitochondrial Phylogenomics Using Four New Mitochondrial Genome Assemblies
Mitochondrial genomes offer pragmatic genetic markers to reconstruct evolutionary relationships and inform taxonomic classifications. Here, we present complete mitochondrial sequences for four Chinese pygmy grasshoppers (Tetrigidae), aiming to reevaluate phylogenetic patterns and morphological taxon...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/15/3/174 |
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| author | De-Long Guan Chao-Mei Huang Wei-An Deng |
| author_facet | De-Long Guan Chao-Mei Huang Wei-An Deng |
| author_sort | De-Long Guan |
| collection | DOAJ |
| description | Mitochondrial genomes offer pragmatic genetic markers to reconstruct evolutionary relationships and inform taxonomic classifications. Here, we present complete mitochondrial sequences for four Chinese pygmy grasshoppers (Tetrigidae), aiming to reevaluate phylogenetic patterns and morphological taxonomy. Our 17,643 bp, 16,274 bp, 15,086 bp, and 15,398 bp mitogenomes of <i>Exothotettix guangxiensis</i>, <i>Formosatettix longwangshanensis</i>, <i>Euparatettix sinufemoralis</i> and <i>Systolederus zhengi</i>, respectively, exhibit archetypal Tetrigidae architecture. We constructed phylogenies using 13 protein-coding loci from 39 Tetrigidae mitogenomes, revealing several genus-level clusters with statistically solid support, conflicts regarding <i>Ex. guangxiensis</i>, <i>F. longwangshanensis</i> merging into <i>Tetrix</i>, and two subclades of <i>Systolederus</i>. The dated divergence analysis indicates over 150 Mya of Tetrigidae ancestry, tracing the <i>Systolederus</i> generic group splits up to ~75 million years ago. Moreover, the <i>Tetrix</i> generic group radiated over 14 Mya across vast distributions, consistent with rapid adaptive dispersals. Our mitochondrial reconstructions suggest that <i>Synstolederus</i> is taxonomically overextended for a single genus, while the distinctiveness of <i>Ex. guangxiensis</i> and <i>F. longwangshanensis</i> from <i>Tetrix</i> appears questionable, and the <i>Tetrix</i> generic group comprises a potential tRNA-Ile coding region. Our integrative mitogenomic approaches will help resolve issues stemming from morphological taxonomy that is reliant on traits that are prone to convergence. This investigation enhances comprehension of Tetrigidae phylogeny and accentuates molecular systematics. |
| first_indexed | 2024-04-24T18:10:33Z |
| format | Article |
| id | doaj.art-b3e5ecfd901c4684a046ba317a8c908e |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-04-24T18:10:33Z |
| publishDate | 2024-03-01 |
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| record_format | Article |
| series | Insects |
| spelling | doaj.art-b3e5ecfd901c4684a046ba317a8c908e2024-03-27T13:47:15ZengMDPI AGInsects2075-44502024-03-0115317410.3390/insects15030174Reassessment of the Phylogenetics of Two Pygmy Grasshopper Generic Groups <i>Tetrix</i> and <i>Systolederus</i> through Mitochondrial Phylogenomics Using Four New Mitochondrial Genome AssembliesDe-Long Guan0Chao-Mei Huang1Wei-An Deng2Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin 541006, ChinaKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin 541006, ChinaKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin 541006, ChinaMitochondrial genomes offer pragmatic genetic markers to reconstruct evolutionary relationships and inform taxonomic classifications. Here, we present complete mitochondrial sequences for four Chinese pygmy grasshoppers (Tetrigidae), aiming to reevaluate phylogenetic patterns and morphological taxonomy. Our 17,643 bp, 16,274 bp, 15,086 bp, and 15,398 bp mitogenomes of <i>Exothotettix guangxiensis</i>, <i>Formosatettix longwangshanensis</i>, <i>Euparatettix sinufemoralis</i> and <i>Systolederus zhengi</i>, respectively, exhibit archetypal Tetrigidae architecture. We constructed phylogenies using 13 protein-coding loci from 39 Tetrigidae mitogenomes, revealing several genus-level clusters with statistically solid support, conflicts regarding <i>Ex. guangxiensis</i>, <i>F. longwangshanensis</i> merging into <i>Tetrix</i>, and two subclades of <i>Systolederus</i>. The dated divergence analysis indicates over 150 Mya of Tetrigidae ancestry, tracing the <i>Systolederus</i> generic group splits up to ~75 million years ago. Moreover, the <i>Tetrix</i> generic group radiated over 14 Mya across vast distributions, consistent with rapid adaptive dispersals. Our mitochondrial reconstructions suggest that <i>Synstolederus</i> is taxonomically overextended for a single genus, while the distinctiveness of <i>Ex. guangxiensis</i> and <i>F. longwangshanensis</i> from <i>Tetrix</i> appears questionable, and the <i>Tetrix</i> generic group comprises a potential tRNA-Ile coding region. Our integrative mitogenomic approaches will help resolve issues stemming from morphological taxonomy that is reliant on traits that are prone to convergence. This investigation enhances comprehension of Tetrigidae phylogeny and accentuates molecular systematics.https://www.mdpi.com/2075-4450/15/3/174mitochondrial genomepygmy grasshoppersTetrigidaephylogenytaxonomic revisiondivergence dating |
| spellingShingle | De-Long Guan Chao-Mei Huang Wei-An Deng Reassessment of the Phylogenetics of Two Pygmy Grasshopper Generic Groups <i>Tetrix</i> and <i>Systolederus</i> through Mitochondrial Phylogenomics Using Four New Mitochondrial Genome Assemblies Insects mitochondrial genome pygmy grasshoppers Tetrigidae phylogeny taxonomic revision divergence dating |
| title | Reassessment of the Phylogenetics of Two Pygmy Grasshopper Generic Groups <i>Tetrix</i> and <i>Systolederus</i> through Mitochondrial Phylogenomics Using Four New Mitochondrial Genome Assemblies |
| title_full | Reassessment of the Phylogenetics of Two Pygmy Grasshopper Generic Groups <i>Tetrix</i> and <i>Systolederus</i> through Mitochondrial Phylogenomics Using Four New Mitochondrial Genome Assemblies |
| title_fullStr | Reassessment of the Phylogenetics of Two Pygmy Grasshopper Generic Groups <i>Tetrix</i> and <i>Systolederus</i> through Mitochondrial Phylogenomics Using Four New Mitochondrial Genome Assemblies |
| title_full_unstemmed | Reassessment of the Phylogenetics of Two Pygmy Grasshopper Generic Groups <i>Tetrix</i> and <i>Systolederus</i> through Mitochondrial Phylogenomics Using Four New Mitochondrial Genome Assemblies |
| title_short | Reassessment of the Phylogenetics of Two Pygmy Grasshopper Generic Groups <i>Tetrix</i> and <i>Systolederus</i> through Mitochondrial Phylogenomics Using Four New Mitochondrial Genome Assemblies |
| title_sort | reassessment of the phylogenetics of two pygmy grasshopper generic groups i tetrix i and i systolederus i through mitochondrial phylogenomics using four new mitochondrial genome assemblies |
| topic | mitochondrial genome pygmy grasshoppers Tetrigidae phylogeny taxonomic revision divergence dating |
| url | https://www.mdpi.com/2075-4450/15/3/174 |
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