Synergistic Photoantimicrobial Chemotherapy of Methylene Blue-Encapsulated Chitosan on Biofilm-Contaminated Titanium
Intensive efforts have been made to eliminate or substantial reduce bacterial adhesion and biofilm formation on titanium implants. However, in the management of peri-implantitis, the methylene blue (MB) photosensitizer commonly used in photoantimicrobial chemotherapy (PACT) is limited to a low reten...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-04-01
|
| Series: | Pharmaceuticals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8247/14/4/346 |
| _version_ | 1827695580093087744 |
|---|---|
| author | Chiu-Nan Lin Shinn-Jyh Ding Chun-Cheng Chen |
| author_facet | Chiu-Nan Lin Shinn-Jyh Ding Chun-Cheng Chen |
| author_sort | Chiu-Nan Lin |
| collection | DOAJ |
| description | Intensive efforts have been made to eliminate or substantial reduce bacterial adhesion and biofilm formation on titanium implants. However, in the management of peri-implantitis, the methylene blue (MB) photosensitizer commonly used in photoantimicrobial chemotherapy (PACT) is limited to a low retention on the implant surface. The purpose of this study was to assess enhancive effect of water-soluble quaternary ammonium chitosan (QTS) on MB retention on biofilm-infected SLA (sandblasted, large grid, and acid-etched) Ti alloy surfaces in vitro. The effectiveness of QTS + MB with different concentrations in eliminating Gram-negative <i>A. actinomycetemcomitans</i> or Gram-positive <i>S. mutans</i> bacteria was compared before and after PACT. Bacterial counting and lipopolysaccharide (LPS) detection were examined, and then the growth of human osteoblast-like MG63 cells was evaluated. The results indicated that the synergistic QTS + MB with retention ability significantly decreased the biofilm accumulation on the Ti alloy surface, which was better than the same concentration of 1 wt% methyl cellulose (MC). More importantly, the osteogenic activity of MG63 cells on the disinfected sample treated by QTS + MB-PACT modality was comparable to that of sterile Ti control, significantly higher than that by MC + MB-PACT modality. It is concluded that, in terms of improved retention efficacy, effective bacteria eradication, and enhanced cell growth, synergistically, PACT using the 100 μg/mL MB-encapsulated 1% QTS was a promising modality for the treatment of peri-implantitis. |
| first_indexed | 2024-03-10T12:29:15Z |
| format | Article |
| id | doaj.art-b3e82013583c4a4aa102eeb47697a694 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T12:29:15Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-b3e82013583c4a4aa102eeb47697a6942023-11-21T14:50:02ZengMDPI AGPharmaceuticals1424-82472021-04-0114434610.3390/ph14040346Synergistic Photoantimicrobial Chemotherapy of Methylene Blue-Encapsulated Chitosan on Biofilm-Contaminated TitaniumChiu-Nan Lin0Shinn-Jyh Ding1Chun-Cheng Chen2Institute of Oral Science, Chung Shan Medical University, Taichung City 402, TaiwanInstitute of Oral Science, Chung Shan Medical University, Taichung City 402, TaiwanDepartment of Stomatology, Chung Shan Medical University Hospital, Taichung City 402, TaiwanIntensive efforts have been made to eliminate or substantial reduce bacterial adhesion and biofilm formation on titanium implants. However, in the management of peri-implantitis, the methylene blue (MB) photosensitizer commonly used in photoantimicrobial chemotherapy (PACT) is limited to a low retention on the implant surface. The purpose of this study was to assess enhancive effect of water-soluble quaternary ammonium chitosan (QTS) on MB retention on biofilm-infected SLA (sandblasted, large grid, and acid-etched) Ti alloy surfaces in vitro. The effectiveness of QTS + MB with different concentrations in eliminating Gram-negative <i>A. actinomycetemcomitans</i> or Gram-positive <i>S. mutans</i> bacteria was compared before and after PACT. Bacterial counting and lipopolysaccharide (LPS) detection were examined, and then the growth of human osteoblast-like MG63 cells was evaluated. The results indicated that the synergistic QTS + MB with retention ability significantly decreased the biofilm accumulation on the Ti alloy surface, which was better than the same concentration of 1 wt% methyl cellulose (MC). More importantly, the osteogenic activity of MG63 cells on the disinfected sample treated by QTS + MB-PACT modality was comparable to that of sterile Ti control, significantly higher than that by MC + MB-PACT modality. It is concluded that, in terms of improved retention efficacy, effective bacteria eradication, and enhanced cell growth, synergistically, PACT using the 100 μg/mL MB-encapsulated 1% QTS was a promising modality for the treatment of peri-implantitis.https://www.mdpi.com/1424-8247/14/4/346dental implantperi-implantitisphotoantimicrobial chemotherapymethylene bluechitosan |
| spellingShingle | Chiu-Nan Lin Shinn-Jyh Ding Chun-Cheng Chen Synergistic Photoantimicrobial Chemotherapy of Methylene Blue-Encapsulated Chitosan on Biofilm-Contaminated Titanium Pharmaceuticals dental implant peri-implantitis photoantimicrobial chemotherapy methylene blue chitosan |
| title | Synergistic Photoantimicrobial Chemotherapy of Methylene Blue-Encapsulated Chitosan on Biofilm-Contaminated Titanium |
| title_full | Synergistic Photoantimicrobial Chemotherapy of Methylene Blue-Encapsulated Chitosan on Biofilm-Contaminated Titanium |
| title_fullStr | Synergistic Photoantimicrobial Chemotherapy of Methylene Blue-Encapsulated Chitosan on Biofilm-Contaminated Titanium |
| title_full_unstemmed | Synergistic Photoantimicrobial Chemotherapy of Methylene Blue-Encapsulated Chitosan on Biofilm-Contaminated Titanium |
| title_short | Synergistic Photoantimicrobial Chemotherapy of Methylene Blue-Encapsulated Chitosan on Biofilm-Contaminated Titanium |
| title_sort | synergistic photoantimicrobial chemotherapy of methylene blue encapsulated chitosan on biofilm contaminated titanium |
| topic | dental implant peri-implantitis photoantimicrobial chemotherapy methylene blue chitosan |
| url | https://www.mdpi.com/1424-8247/14/4/346 |
| work_keys_str_mv | AT chiunanlin synergisticphotoantimicrobialchemotherapyofmethyleneblueencapsulatedchitosanonbiofilmcontaminatedtitanium AT shinnjyhding synergisticphotoantimicrobialchemotherapyofmethyleneblueencapsulatedchitosanonbiofilmcontaminatedtitanium AT chunchengchen synergisticphotoantimicrobialchemotherapyofmethyleneblueencapsulatedchitosanonbiofilmcontaminatedtitanium |