Effects of Hydrogen-Rich Water on Postharvest Physiology in Scales of Lanzhou Lily during Storage
Hydrogen gas (H<sub>2</sub>) is considered as a signaling molecule and plays multiple roles in plant growth. However, the effect of H<sub>2</sub> on postharvest physiology in lily scales during storage has not been reported. In this study, the regulatory roles of hydrogen-ric...
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MDPI AG
2023-01-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/2/156 |
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| author | Xingjuan Liu Hua Fang Panpan Huang Li Feng Fujin Ye Lijuan Wei Xuetong Wu Hongsheng Zhang Weibiao Liao |
| author_facet | Xingjuan Liu Hua Fang Panpan Huang Li Feng Fujin Ye Lijuan Wei Xuetong Wu Hongsheng Zhang Weibiao Liao |
| author_sort | Xingjuan Liu |
| collection | DOAJ |
| description | Hydrogen gas (H<sub>2</sub>) is considered as a signaling molecule and plays multiple roles in plant growth. However, the effect of H<sub>2</sub> on postharvest physiology in lily scales during storage has not been reported. In this study, the regulatory roles of hydrogen-rich water (HRW, a H<sub>2</sub> donor, a concentration of 0.45 mM for 100% HRW) in water status, ion balance, and nutrients in Lanzhou lily (<i>Lilium davidii</i> var. <i>unicolor</i>) scales were investigated. The scales were soaked in HRW for 12 d, and sampling was performed every 3 d for a total of 5 times. The results show that HRW (0, 10, 50, and 100%) increased the fresh weight, dry weight, relative water content, and water loss rate in lily scales, with maximum biological response at 50% HRW. Treatment with 50% HRW significantly increased the K<sup>+</sup> content and K<sup>+</sup>/Na<sup>+</sup> ratio in lily scales and decreased Na<sup>+</sup> content. The Na<sup>+</sup> K<sup>+</sup>-ATPase, and PM H<sup>+</sup>-ATPase activities were also increased by 50% HRW treatment. Meanwhile, 50% HRW up-regulated the expression of <i>AKT1</i> and <i>HA3</i> genes and down-regulated the expression of <i>NHX2</i> and <i>SOS1</i> genes. In addition, 50% HRW treatment significantly increased the expression level of <i>PIP1;5</i>, <i>PIP2A</i>, <i>TIP1;3</i>, and <i>TIP2;2</i> genes. Treatment with 50% HRW significantly increased the content of water-soluble carbohydrate, sucrose, glucose, and fructose in lily scales, and decreased the content of starch. In addition, 50% HRW treatment significantly increased the activity of α-amylase, β-amylase, total amylase, sucrose synthase, and sucrose phosphate synthase. Collectively, H<sub>2</sub> might enhance the water retention capacity and nutrient content in lily scales by maintaining ion balance, regulating aquaporin, and increasing sugar-metabolizing enzyme activity, thereby prolonging the storage period of postharvest scales of Lanzhou lily. |
| first_indexed | 2024-03-11T08:46:09Z |
| format | Article |
| id | doaj.art-b3e8a970a23548e7a467a78f2ecd57f3 |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-11T08:46:09Z |
| publishDate | 2023-01-01 |
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| spelling | doaj.art-b3e8a970a23548e7a467a78f2ecd57f32023-11-16T20:49:24ZengMDPI AGHorticulturae2311-75242023-01-019215610.3390/horticulturae9020156Effects of Hydrogen-Rich Water on Postharvest Physiology in Scales of Lanzhou Lily during StorageXingjuan Liu0Hua Fang1Panpan Huang2Li Feng3Fujin Ye4Lijuan Wei5Xuetong Wu6Hongsheng Zhang7Weibiao Liao8College of Horticulture, Gansu Agricultural University, 1 Yinmen Village, Anning District, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, 1 Yinmen Village, Anning District, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, 1 Yinmen Village, Anning District, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, 1 Yinmen Village, Anning District, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, 1 Yinmen Village, Anning District, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, 1 Yinmen Village, Anning District, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, 1 Yinmen Village, Anning District, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, 1 Yinmen Village, Anning District, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, 1 Yinmen Village, Anning District, Lanzhou 730070, ChinaHydrogen gas (H<sub>2</sub>) is considered as a signaling molecule and plays multiple roles in plant growth. However, the effect of H<sub>2</sub> on postharvest physiology in lily scales during storage has not been reported. In this study, the regulatory roles of hydrogen-rich water (HRW, a H<sub>2</sub> donor, a concentration of 0.45 mM for 100% HRW) in water status, ion balance, and nutrients in Lanzhou lily (<i>Lilium davidii</i> var. <i>unicolor</i>) scales were investigated. The scales were soaked in HRW for 12 d, and sampling was performed every 3 d for a total of 5 times. The results show that HRW (0, 10, 50, and 100%) increased the fresh weight, dry weight, relative water content, and water loss rate in lily scales, with maximum biological response at 50% HRW. Treatment with 50% HRW significantly increased the K<sup>+</sup> content and K<sup>+</sup>/Na<sup>+</sup> ratio in lily scales and decreased Na<sup>+</sup> content. The Na<sup>+</sup> K<sup>+</sup>-ATPase, and PM H<sup>+</sup>-ATPase activities were also increased by 50% HRW treatment. Meanwhile, 50% HRW up-regulated the expression of <i>AKT1</i> and <i>HA3</i> genes and down-regulated the expression of <i>NHX2</i> and <i>SOS1</i> genes. In addition, 50% HRW treatment significantly increased the expression level of <i>PIP1;5</i>, <i>PIP2A</i>, <i>TIP1;3</i>, and <i>TIP2;2</i> genes. Treatment with 50% HRW significantly increased the content of water-soluble carbohydrate, sucrose, glucose, and fructose in lily scales, and decreased the content of starch. In addition, 50% HRW treatment significantly increased the activity of α-amylase, β-amylase, total amylase, sucrose synthase, and sucrose phosphate synthase. Collectively, H<sub>2</sub> might enhance the water retention capacity and nutrient content in lily scales by maintaining ion balance, regulating aquaporin, and increasing sugar-metabolizing enzyme activity, thereby prolonging the storage period of postharvest scales of Lanzhou lily.https://www.mdpi.com/2311-7524/9/2/156hydrogen-rich water (HRW)lily scaleswater lossionic equilibriumaquaporinnutrients |
| spellingShingle | Xingjuan Liu Hua Fang Panpan Huang Li Feng Fujin Ye Lijuan Wei Xuetong Wu Hongsheng Zhang Weibiao Liao Effects of Hydrogen-Rich Water on Postharvest Physiology in Scales of Lanzhou Lily during Storage Horticulturae hydrogen-rich water (HRW) lily scales water loss ionic equilibrium aquaporin nutrients |
| title | Effects of Hydrogen-Rich Water on Postharvest Physiology in Scales of Lanzhou Lily during Storage |
| title_full | Effects of Hydrogen-Rich Water on Postharvest Physiology in Scales of Lanzhou Lily during Storage |
| title_fullStr | Effects of Hydrogen-Rich Water on Postharvest Physiology in Scales of Lanzhou Lily during Storage |
| title_full_unstemmed | Effects of Hydrogen-Rich Water on Postharvest Physiology in Scales of Lanzhou Lily during Storage |
| title_short | Effects of Hydrogen-Rich Water on Postharvest Physiology in Scales of Lanzhou Lily during Storage |
| title_sort | effects of hydrogen rich water on postharvest physiology in scales of lanzhou lily during storage |
| topic | hydrogen-rich water (HRW) lily scales water loss ionic equilibrium aquaporin nutrients |
| url | https://www.mdpi.com/2311-7524/9/2/156 |
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