Zika virus isolation, propagation, and quantification using multiple methods.

Zika virus isolation, propagation, and quantification using multiple methods.

Zika virus (ZIKV) was isolated from the archival urine, serum, and autopsy specimens by intrathoracic inoculation of Toxorhynchitis splendens and followed by three blind sub-passaging in C6/36 mosquito cells. The virus isolates were identified using an immunofluorescence assay and real-time reverse...

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Main Authors: Worawat Dangsagul, Kriengsak Ruchusatsawat, Apiwat Tawatsin, Don Changsom, Pirom Noisumdaeng, Sukontip Putchakarn, Chayawat Phatihattakorn, Prasert Auewarakul, Pilaipan Puthavathana
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2021-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0255314

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