The Effect of Visible Light on Cell Envelope Subproteome during <i>Vibrio harveyi</i> Survival at 20 °C in Seawater
A number of <i>Vibrio</i> spp. belong to the well-studied model organisms used to understand the strategies developed by marine bacteria to cope with adverse conditions (starvation, suboptimal temperature, solar radiation, etc.) in their natural environments. Temperature and nutrient ava...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-03-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/9/3/594 |
| _version_ | 1827697791188598784 |
|---|---|
| author | Maite Orruño Claudia Parada Vladimir R. Kaberdin Inés Arana |
| author_facet | Maite Orruño Claudia Parada Vladimir R. Kaberdin Inés Arana |
| author_sort | Maite Orruño |
| collection | DOAJ |
| description | A number of <i>Vibrio</i> spp. belong to the well-studied model organisms used to understand the strategies developed by marine bacteria to cope with adverse conditions (starvation, suboptimal temperature, solar radiation, etc.) in their natural environments. Temperature and nutrient availability are considered to be the key factors that influence <i>Vibrio harveyi</i> physiology, morphology, and persistence in aquatic systems. In contrast to the well-studied effects of temperature and starvation on <i>Vibrio</i> survival, little is known about the impact of visible light able to cause photooxidative stress. Here we employ <i>V. harveyi</i> ATCC 14126<sup>T</sup> as a model organism to analyze and compare the survival patterns and changes in the protein composition of its cell envelope during the long-term permanence of this bacterium in seawater microcosm at 20 °C in the presence and absence of illumination with visible light. We found that <i>V. harveyi</i> exposure to visible light reduces cell culturability likely inducing the entry into the Viable but Non Culturable state (VBNC), whereas populations maintained in darkness remained culturable for at least 21 days. Despite these differences, the starved cells in both populations underwent morphological changes by reducing their size. Moreover, further proteomic analysis revealed a number of changes in the composition of cell envelope potentially accountable for the different adaptation pattern manifested in the absence and presence of visible light. |
| first_indexed | 2024-03-10T13:16:44Z |
| format | Article |
| id | doaj.art-b3eb77d277a947ccb7c8390a303d232b |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T13:16:44Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-b3eb77d277a947ccb7c8390a303d232b2023-11-21T10:22:52ZengMDPI AGMicroorganisms2076-26072021-03-019359410.3390/microorganisms9030594The Effect of Visible Light on Cell Envelope Subproteome during <i>Vibrio harveyi</i> Survival at 20 °C in SeawaterMaite Orruño0Claudia Parada1Vladimir R. Kaberdin2Inés Arana3Department of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country UPV/EHU, 48340 Leioa, SpainDepartment of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country UPV/EHU, 48340 Leioa, SpainDepartment of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country UPV/EHU, 48340 Leioa, SpainDepartment of Immunology, Microbiology and Parasitology, Faculty of Science and Technology, University of the Basque Country UPV/EHU, 48340 Leioa, SpainA number of <i>Vibrio</i> spp. belong to the well-studied model organisms used to understand the strategies developed by marine bacteria to cope with adverse conditions (starvation, suboptimal temperature, solar radiation, etc.) in their natural environments. Temperature and nutrient availability are considered to be the key factors that influence <i>Vibrio harveyi</i> physiology, morphology, and persistence in aquatic systems. In contrast to the well-studied effects of temperature and starvation on <i>Vibrio</i> survival, little is known about the impact of visible light able to cause photooxidative stress. Here we employ <i>V. harveyi</i> ATCC 14126<sup>T</sup> as a model organism to analyze and compare the survival patterns and changes in the protein composition of its cell envelope during the long-term permanence of this bacterium in seawater microcosm at 20 °C in the presence and absence of illumination with visible light. We found that <i>V. harveyi</i> exposure to visible light reduces cell culturability likely inducing the entry into the Viable but Non Culturable state (VBNC), whereas populations maintained in darkness remained culturable for at least 21 days. Despite these differences, the starved cells in both populations underwent morphological changes by reducing their size. Moreover, further proteomic analysis revealed a number of changes in the composition of cell envelope potentially accountable for the different adaptation pattern manifested in the absence and presence of visible light.https://www.mdpi.com/2076-2607/9/3/594<i>Vibrio</i>seawaterstarvationvisible lightmembrane subproteome |
| spellingShingle | Maite Orruño Claudia Parada Vladimir R. Kaberdin Inés Arana The Effect of Visible Light on Cell Envelope Subproteome during <i>Vibrio harveyi</i> Survival at 20 °C in Seawater Microorganisms <i>Vibrio</i> seawater starvation visible light membrane subproteome |
| title | The Effect of Visible Light on Cell Envelope Subproteome during <i>Vibrio harveyi</i> Survival at 20 °C in Seawater |
| title_full | The Effect of Visible Light on Cell Envelope Subproteome during <i>Vibrio harveyi</i> Survival at 20 °C in Seawater |
| title_fullStr | The Effect of Visible Light on Cell Envelope Subproteome during <i>Vibrio harveyi</i> Survival at 20 °C in Seawater |
| title_full_unstemmed | The Effect of Visible Light on Cell Envelope Subproteome during <i>Vibrio harveyi</i> Survival at 20 °C in Seawater |
| title_short | The Effect of Visible Light on Cell Envelope Subproteome during <i>Vibrio harveyi</i> Survival at 20 °C in Seawater |
| title_sort | effect of visible light on cell envelope subproteome during i vibrio harveyi i survival at 20 °c in seawater |
| topic | <i>Vibrio</i> seawater starvation visible light membrane subproteome |
| url | https://www.mdpi.com/2076-2607/9/3/594 |
| work_keys_str_mv | AT maiteorruno theeffectofvisiblelightoncellenvelopesubproteomeduringivibrioharveyiisurvivalat20cinseawater AT claudiaparada theeffectofvisiblelightoncellenvelopesubproteomeduringivibrioharveyiisurvivalat20cinseawater AT vladimirrkaberdin theeffectofvisiblelightoncellenvelopesubproteomeduringivibrioharveyiisurvivalat20cinseawater AT inesarana theeffectofvisiblelightoncellenvelopesubproteomeduringivibrioharveyiisurvivalat20cinseawater AT maiteorruno effectofvisiblelightoncellenvelopesubproteomeduringivibrioharveyiisurvivalat20cinseawater AT claudiaparada effectofvisiblelightoncellenvelopesubproteomeduringivibrioharveyiisurvivalat20cinseawater AT vladimirrkaberdin effectofvisiblelightoncellenvelopesubproteomeduringivibrioharveyiisurvivalat20cinseawater AT inesarana effectofvisiblelightoncellenvelopesubproteomeduringivibrioharveyiisurvivalat20cinseawater |