Additive effect of the probiotics Lactobacillus exopolysaccharides and the Satureja calamintha extracts on enteropathogenic Escherichia coli adhesion
Abstract This study assessed the inhibitory potential of the probiotics Lactobacillus (LB) exopolysaccharides (EPS) with or without extracts of Satureja calamintha on enteropathogenic Escherichia coli (EPEc) responsible for gastroenteritis. Methanolic and hydromethanolic extracts were prepared by co...
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2022-11-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100759&tlng=en |
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| author | Hamida Benfreha Emanuella Chiara Valença Pereira Larissa Araújo Rolim Nadia Chelli Jackson Roberto Guedes da Silva Almeida Aicha Tirtouil Boumediène Meddah |
| author_facet | Hamida Benfreha Emanuella Chiara Valença Pereira Larissa Araújo Rolim Nadia Chelli Jackson Roberto Guedes da Silva Almeida Aicha Tirtouil Boumediène Meddah |
| author_sort | Hamida Benfreha |
| collection | DOAJ |
| description | Abstract This study assessed the inhibitory potential of the probiotics Lactobacillus (LB) exopolysaccharides (EPS) with or without extracts of Satureja calamintha on enteropathogenic Escherichia coli (EPEc) responsible for gastroenteritis. Methanolic and hydromethanolic extracts were prepared by cold maceration and subjected to phytochemical screening. The compounds of the extracts were determined with the colorimetric assays and identified using high-performance liquid chromatography coupled with diode array detector (HPLC-DAD). Antioxidant activities of the extracts were also evaluated by using 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging. Antibacterial effect on EPEc was evaluated by using both agar disc diffusion and microdilution methods. The in vitro test of auto-aggregation was investigated. Microbiological analysis showed that 63% of the isolated LB were producing EPS, with the amount ranging from 8.21 to 43.13 mg/L. Chemical analysis of the extracts revealed the presence of polyphenols and flavonoids, more abundant in the hydromethanolic extract, which presented the highest content with 2.11 mg EGA/g of polyphenol and 1.64 mg EC/g of flavonoids and 1.71 mg EGA/g of polyphenol and 1.15 mg EC/g of flavonoids in the methanolic extract. Hydromethanolic extracts and EPS exhibited a more important activity than did the methanolic extract against EPEc. The combined action of EPS and extracts reduced the aggregation ability of EPEc and decreased the rate of their adhesion. |
| first_indexed | 2024-04-13T13:34:57Z |
| format | Article |
| id | doaj.art-b3ebaa51e79345119f0b9b0f1076e62d |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-04-13T13:34:57Z |
| publishDate | 2022-11-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-b3ebaa51e79345119f0b9b0f1076e62d2022-12-22T02:44:48ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902022-11-015810.1590/s2175-97902022e20015Additive effect of the probiotics Lactobacillus exopolysaccharides and the Satureja calamintha extracts on enteropathogenic Escherichia coli adhesionHamida BenfrehaEmanuella Chiara Valença PereiraLarissa Araújo RolimNadia ChelliJackson Roberto Guedes da Silva Almeidahttps://orcid.org/0000-0002-0867-1357Aicha TirtouilBoumediène MeddahAbstract This study assessed the inhibitory potential of the probiotics Lactobacillus (LB) exopolysaccharides (EPS) with or without extracts of Satureja calamintha on enteropathogenic Escherichia coli (EPEc) responsible for gastroenteritis. Methanolic and hydromethanolic extracts were prepared by cold maceration and subjected to phytochemical screening. The compounds of the extracts were determined with the colorimetric assays and identified using high-performance liquid chromatography coupled with diode array detector (HPLC-DAD). Antioxidant activities of the extracts were also evaluated by using 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging. Antibacterial effect on EPEc was evaluated by using both agar disc diffusion and microdilution methods. The in vitro test of auto-aggregation was investigated. Microbiological analysis showed that 63% of the isolated LB were producing EPS, with the amount ranging from 8.21 to 43.13 mg/L. Chemical analysis of the extracts revealed the presence of polyphenols and flavonoids, more abundant in the hydromethanolic extract, which presented the highest content with 2.11 mg EGA/g of polyphenol and 1.64 mg EC/g of flavonoids and 1.71 mg EGA/g of polyphenol and 1.15 mg EC/g of flavonoids in the methanolic extract. Hydromethanolic extracts and EPS exhibited a more important activity than did the methanolic extract against EPEc. The combined action of EPS and extracts reduced the aggregation ability of EPEc and decreased the rate of their adhesion.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100759&tlng=enSatureja calamintha subspNepetaExtractEPEcProbioticsExopolysaccharides |
| spellingShingle | Hamida Benfreha Emanuella Chiara Valença Pereira Larissa Araújo Rolim Nadia Chelli Jackson Roberto Guedes da Silva Almeida Aicha Tirtouil Boumediène Meddah Additive effect of the probiotics Lactobacillus exopolysaccharides and the Satureja calamintha extracts on enteropathogenic Escherichia coli adhesion Brazilian Journal of Pharmaceutical Sciences Satureja calamintha subsp Nepeta Extract EPEc Probiotics Exopolysaccharides |
| title | Additive effect of the probiotics Lactobacillus exopolysaccharides and the Satureja calamintha extracts on enteropathogenic Escherichia coli adhesion |
| title_full | Additive effect of the probiotics Lactobacillus exopolysaccharides and the Satureja calamintha extracts on enteropathogenic Escherichia coli adhesion |
| title_fullStr | Additive effect of the probiotics Lactobacillus exopolysaccharides and the Satureja calamintha extracts on enteropathogenic Escherichia coli adhesion |
| title_full_unstemmed | Additive effect of the probiotics Lactobacillus exopolysaccharides and the Satureja calamintha extracts on enteropathogenic Escherichia coli adhesion |
| title_short | Additive effect of the probiotics Lactobacillus exopolysaccharides and the Satureja calamintha extracts on enteropathogenic Escherichia coli adhesion |
| title_sort | additive effect of the probiotics lactobacillus exopolysaccharides and the satureja calamintha extracts on enteropathogenic escherichia coli adhesion |
| topic | Satureja calamintha subsp Nepeta Extract EPEc Probiotics Exopolysaccharides |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100759&tlng=en |
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