Analysis of miRNA‐mRNA regulatory network revealed key genes induced by aflatoxin B1 exposure in primary human hepatocytes
Abstract Background Aflatoxin B1 (AFB1) exposure is a crucial factor to initiate hepatocellular carcinoma (HCC). However, comprehensive microRNA (miRNA)‐message RNA (mRNA) regulatory network regarding AFB1‐associated HCC is still lacking. This work was aimed to identify miRNA‐mRNA network in primary...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-11-01
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| Series: | Molecular Genetics & Genomic Medicine |
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| Online Access: | https://doi.org/10.1002/mgg3.971 |
| _version_ | 1797296159609323520 |
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| author | Ziyang Zhang Dongyang Tang Bin Wang Zhiwei Wang Mingjiu Liu |
| author_facet | Ziyang Zhang Dongyang Tang Bin Wang Zhiwei Wang Mingjiu Liu |
| author_sort | Ziyang Zhang |
| collection | DOAJ |
| description | Abstract Background Aflatoxin B1 (AFB1) exposure is a crucial factor to initiate hepatocellular carcinoma (HCC). However, comprehensive microRNA (miRNA)‐message RNA (mRNA) regulatory network regarding AFB1‐associated HCC is still lacking. This work was aimed to identify miRNA‐mRNA network in primary human hepatocytes after AFB1 exposure. Methods A miRNA expression dataset GSE71540 obtained from the gene expression omnibus (GEO) was used to identify differentially expressed miRNAs (DEMs) after AFB1 exposure using GEO2R. Target genes of these DEMs were identified using TargetScan V_7.2, miRDB, PITA, miRanda, and miRTarBase. Gene ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed at Database for Annotation, Visualization and Integrated Discovery (DAVID). miRNA‐mRNA regulatory network was established by analyzing three enriched KEGG pathways significantly correlated with HCC onset and then visualized at CytoScape. Results In this work, nine upregulated and nine downregulated DEMs were identified. Functional enrichment analyses showed that these predicted target genes were significantly associated with cancer development. Analysis of three enriched pathways related to the onset of HCC identified 13 and nine target genes for upregulated DEMs and downregulated DEMs, respectively. Subsequently, the miRNA‐mRNA regulatory networks were constructed. Conclusions In conclusion, miRNA‐mRNA regulatory network was established, which will help to understand the mechanism underlying the AFB1‐induced onset of HCC. |
| first_indexed | 2024-03-07T21:59:21Z |
| format | Article |
| id | doaj.art-b3ecb70c86dc415684a47d547e63018e |
| institution | Directory Open Access Journal |
| issn | 2324-9269 |
| language | English |
| last_indexed | 2024-03-07T21:59:21Z |
| publishDate | 2019-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Molecular Genetics & Genomic Medicine |
| spelling | doaj.art-b3ecb70c86dc415684a47d547e63018e2024-02-24T07:01:11ZengWileyMolecular Genetics & Genomic Medicine2324-92692019-11-01711n/an/a10.1002/mgg3.971Analysis of miRNA‐mRNA regulatory network revealed key genes induced by aflatoxin B1 exposure in primary human hepatocytesZiyang Zhang0Dongyang Tang1Bin Wang2Zhiwei Wang3Mingjiu Liu4School of Life Science and Technology Henan Institute of Science and Technology Xinxiang P.R. ChinaDepartment of Experimental Management Center Henan Institute of Science and Technology Xinxiang P.R. ChinaSchool of Life Science and Technology Henan Institute of Science and Technology Xinxiang P.R. ChinaDepartment of Experimental Management Center Henan Institute of Science and Technology Xinxiang P.R. ChinaSchool of Life Science and Technology Henan Institute of Science and Technology Xinxiang P.R. ChinaAbstract Background Aflatoxin B1 (AFB1) exposure is a crucial factor to initiate hepatocellular carcinoma (HCC). However, comprehensive microRNA (miRNA)‐message RNA (mRNA) regulatory network regarding AFB1‐associated HCC is still lacking. This work was aimed to identify miRNA‐mRNA network in primary human hepatocytes after AFB1 exposure. Methods A miRNA expression dataset GSE71540 obtained from the gene expression omnibus (GEO) was used to identify differentially expressed miRNAs (DEMs) after AFB1 exposure using GEO2R. Target genes of these DEMs were identified using TargetScan V_7.2, miRDB, PITA, miRanda, and miRTarBase. Gene ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed at Database for Annotation, Visualization and Integrated Discovery (DAVID). miRNA‐mRNA regulatory network was established by analyzing three enriched KEGG pathways significantly correlated with HCC onset and then visualized at CytoScape. Results In this work, nine upregulated and nine downregulated DEMs were identified. Functional enrichment analyses showed that these predicted target genes were significantly associated with cancer development. Analysis of three enriched pathways related to the onset of HCC identified 13 and nine target genes for upregulated DEMs and downregulated DEMs, respectively. Subsequently, the miRNA‐mRNA regulatory networks were constructed. Conclusions In conclusion, miRNA‐mRNA regulatory network was established, which will help to understand the mechanism underlying the AFB1‐induced onset of HCC.https://doi.org/10.1002/mgg3.971aflatoxin B1hepatocellular carcinomamiRNA/mRNA regulatory network |
| spellingShingle | Ziyang Zhang Dongyang Tang Bin Wang Zhiwei Wang Mingjiu Liu Analysis of miRNA‐mRNA regulatory network revealed key genes induced by aflatoxin B1 exposure in primary human hepatocytes Molecular Genetics & Genomic Medicine aflatoxin B1 hepatocellular carcinoma miRNA/mRNA regulatory network |
| title | Analysis of miRNA‐mRNA regulatory network revealed key genes induced by aflatoxin B1 exposure in primary human hepatocytes |
| title_full | Analysis of miRNA‐mRNA regulatory network revealed key genes induced by aflatoxin B1 exposure in primary human hepatocytes |
| title_fullStr | Analysis of miRNA‐mRNA regulatory network revealed key genes induced by aflatoxin B1 exposure in primary human hepatocytes |
| title_full_unstemmed | Analysis of miRNA‐mRNA regulatory network revealed key genes induced by aflatoxin B1 exposure in primary human hepatocytes |
| title_short | Analysis of miRNA‐mRNA regulatory network revealed key genes induced by aflatoxin B1 exposure in primary human hepatocytes |
| title_sort | analysis of mirna mrna regulatory network revealed key genes induced by aflatoxin b1 exposure in primary human hepatocytes |
| topic | aflatoxin B1 hepatocellular carcinoma miRNA/mRNA regulatory network |
| url | https://doi.org/10.1002/mgg3.971 |
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