Regulation of different phases of AMPA receptor intracellular transport by 4.1N and SAP97
Changes in the number of synaptic AMPA receptors underlie many forms of synaptic plasticity. These variations are controlled by an interplay between their intracellular transport (IT), export to the plasma membrane (PM), stabilization at synapses, and recycling. The cytosolic C-terminal domain of th...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2023-04-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/85609 |
| _version_ | 1827963854310604800 |
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| author | Caroline Bonnet Justine Charpentier Natacha Retailleau Daniel Choquet Françoise Coussen |
| author_facet | Caroline Bonnet Justine Charpentier Natacha Retailleau Daniel Choquet Françoise Coussen |
| author_sort | Caroline Bonnet |
| collection | DOAJ |
| description | Changes in the number of synaptic AMPA receptors underlie many forms of synaptic plasticity. These variations are controlled by an interplay between their intracellular transport (IT), export to the plasma membrane (PM), stabilization at synapses, and recycling. The cytosolic C-terminal domain of the AMPAR GluA1 subunit is specifically associated with 4.1 N and SAP97. We analyze how interactions between GluA1 and 4.1N or SAP97 regulate IT and exocytosis in basal conditions and after cLTP induction. The down-regulation of 4.1N or SAP97 decreases GluA1 IT properties and export to the PM. The total deletion of its C-terminal fully suppresses its IT. Our results demonstrate that during basal transmission, the binding of 4.1N to GluA1 allows their exocytosis whereas the interaction with SAP97 is essential for GluA1 IT. During cLTP, the interaction of 4.1N with GluA1 allows its IT and exocytosis. Our results identify the differential roles of 4.1N and SAP97 in the control of various phases of GluA1 IT. |
| first_indexed | 2024-04-09T17:05:40Z |
| format | Article |
| id | doaj.art-b3eeeb3abc534570a6138355f0d237f9 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-09T17:05:40Z |
| publishDate | 2023-04-01 |
| publisher | eLife Sciences Publications Ltd |
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| series | eLife |
| spelling | doaj.art-b3eeeb3abc534570a6138355f0d237f92023-04-20T15:07:25ZengeLife Sciences Publications LtdeLife2050-084X2023-04-011210.7554/eLife.85609Regulation of different phases of AMPA receptor intracellular transport by 4.1N and SAP97Caroline Bonnet0Justine Charpentier1Natacha Retailleau2Daniel Choquet3https://orcid.org/0000-0003-4726-9763Françoise Coussen4https://orcid.org/0000-0002-3194-3058University of Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, Bordeaux, FranceUniversity of Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, Bordeaux, FranceUniversity of Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, Bordeaux, FranceUniversity of Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, Bordeaux, France; Bordeaux Imaging Center, Bordeaux, FranceUniversity of Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, Bordeaux, FranceChanges in the number of synaptic AMPA receptors underlie many forms of synaptic plasticity. These variations are controlled by an interplay between their intracellular transport (IT), export to the plasma membrane (PM), stabilization at synapses, and recycling. The cytosolic C-terminal domain of the AMPAR GluA1 subunit is specifically associated with 4.1 N and SAP97. We analyze how interactions between GluA1 and 4.1N or SAP97 regulate IT and exocytosis in basal conditions and after cLTP induction. The down-regulation of 4.1N or SAP97 decreases GluA1 IT properties and export to the PM. The total deletion of its C-terminal fully suppresses its IT. Our results demonstrate that during basal transmission, the binding of 4.1N to GluA1 allows their exocytosis whereas the interaction with SAP97 is essential for GluA1 IT. During cLTP, the interaction of 4.1N with GluA1 allows its IT and exocytosis. Our results identify the differential roles of 4.1N and SAP97 in the control of various phases of GluA1 IT.https://elifesciences.org/articles/85609AMPARIntracellular transportLTPSAP974.1N |
| spellingShingle | Caroline Bonnet Justine Charpentier Natacha Retailleau Daniel Choquet Françoise Coussen Regulation of different phases of AMPA receptor intracellular transport by 4.1N and SAP97 eLife AMPAR Intracellular transport LTP SAP97 4.1N |
| title | Regulation of different phases of AMPA receptor intracellular transport by 4.1N and SAP97 |
| title_full | Regulation of different phases of AMPA receptor intracellular transport by 4.1N and SAP97 |
| title_fullStr | Regulation of different phases of AMPA receptor intracellular transport by 4.1N and SAP97 |
| title_full_unstemmed | Regulation of different phases of AMPA receptor intracellular transport by 4.1N and SAP97 |
| title_short | Regulation of different phases of AMPA receptor intracellular transport by 4.1N and SAP97 |
| title_sort | regulation of different phases of ampa receptor intracellular transport by 4 1n and sap97 |
| topic | AMPAR Intracellular transport LTP SAP97 4.1N |
| url | https://elifesciences.org/articles/85609 |
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