Structural Stabilities and Transformation Mechanism of Rhynchophylline and Isorhynchophylline by Ultra Performance Liquid Chromatography/Time-of-Flight Mass Spectrometry (UPLC/Q-TOF-MS)
To reveal the structural stabilities and transformation mechanism of rhynchophylline (RIN) and isorhynchophylline (IRN), HPLC and UPLC-Q-TOF-MS method were developed for the qualitative and quantitative analysis of the conversion rate. The method was validated for linearity, inter- and intra-day pre...
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| Format: | Article |
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MDPI AG
2015-08-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/20/8/14849 |
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| author | Zhen-Feng Wu Ya-Qi Wang Na Wan Gang Ke Peng-Fei Yue Hao Chen Juan-Juan Zhan Ming Yang |
| author_facet | Zhen-Feng Wu Ya-Qi Wang Na Wan Gang Ke Peng-Fei Yue Hao Chen Juan-Juan Zhan Ming Yang |
| author_sort | Zhen-Feng Wu |
| collection | DOAJ |
| description | To reveal the structural stabilities and transformation mechanism of rhynchophylline (RIN) and isorhynchophylline (IRN), HPLC and UPLC-Q-TOF-MS method were developed for the qualitative and quantitative analysis of the conversion rate. The method was validated for linearity, inter- and intra-day precisions, repeatability and stability. All the quantitative determination method validation results were satisfactory. Under the optimized chromatographic conditions, the effect of various heat temperatures, retention time, and solvent polarities on conversion rate and equilibrium were systematically investigated for the first time. Besides, a model relating the retention yield value and time-temperature was built to predict the t0.5 and Ea of the conversion rate by the Arrhenius equation. The experimental results proved to be in good accordance with the predicted values. Furthermore, UPLC-Q-TOF-MS analysis was performed to verify the transformation mechanism and provide valuable information for stability analysis of the conversion products. |
| first_indexed | 2024-12-10T20:31:04Z |
| format | Article |
| id | doaj.art-b3f218d9b5754ab2a2c2dd903788092c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T20:31:04Z |
| publishDate | 2015-08-01 |
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| series | Molecules |
| spelling | doaj.art-b3f218d9b5754ab2a2c2dd903788092c2022-12-22T01:34:42ZengMDPI AGMolecules1420-30492015-08-01208148491485910.3390/molecules200814849molecules200814849Structural Stabilities and Transformation Mechanism of Rhynchophylline and Isorhynchophylline by Ultra Performance Liquid Chromatography/Time-of-Flight Mass Spectrometry (UPLC/Q-TOF-MS)Zhen-Feng Wu0Ya-Qi Wang1Na Wan2Gang Ke3Peng-Fei Yue4Hao Chen5Juan-Juan Zhan6Ming Yang7Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, ChinaKey Laboratory of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, ChinaKey Laboratory of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, ChinaKey Laboratory of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, ChinaKey Laboratory of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, ChinaAffiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, ChinaKey Laboratory of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, ChinaKey Laboratory of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, ChinaTo reveal the structural stabilities and transformation mechanism of rhynchophylline (RIN) and isorhynchophylline (IRN), HPLC and UPLC-Q-TOF-MS method were developed for the qualitative and quantitative analysis of the conversion rate. The method was validated for linearity, inter- and intra-day precisions, repeatability and stability. All the quantitative determination method validation results were satisfactory. Under the optimized chromatographic conditions, the effect of various heat temperatures, retention time, and solvent polarities on conversion rate and equilibrium were systematically investigated for the first time. Besides, a model relating the retention yield value and time-temperature was built to predict the t0.5 and Ea of the conversion rate by the Arrhenius equation. The experimental results proved to be in good accordance with the predicted values. Furthermore, UPLC-Q-TOF-MS analysis was performed to verify the transformation mechanism and provide valuable information for stability analysis of the conversion products.http://www.mdpi.com/1420-3049/20/8/14849rhynchophyllineisorhynchophyllineconversion ratestabilityUPLC-Q-TOF-MS |
| spellingShingle | Zhen-Feng Wu Ya-Qi Wang Na Wan Gang Ke Peng-Fei Yue Hao Chen Juan-Juan Zhan Ming Yang Structural Stabilities and Transformation Mechanism of Rhynchophylline and Isorhynchophylline by Ultra Performance Liquid Chromatography/Time-of-Flight Mass Spectrometry (UPLC/Q-TOF-MS) Molecules rhynchophylline isorhynchophylline conversion rate stability UPLC-Q-TOF-MS |
| title | Structural Stabilities and Transformation Mechanism of Rhynchophylline and Isorhynchophylline by Ultra Performance Liquid Chromatography/Time-of-Flight Mass Spectrometry (UPLC/Q-TOF-MS) |
| title_full | Structural Stabilities and Transformation Mechanism of Rhynchophylline and Isorhynchophylline by Ultra Performance Liquid Chromatography/Time-of-Flight Mass Spectrometry (UPLC/Q-TOF-MS) |
| title_fullStr | Structural Stabilities and Transformation Mechanism of Rhynchophylline and Isorhynchophylline by Ultra Performance Liquid Chromatography/Time-of-Flight Mass Spectrometry (UPLC/Q-TOF-MS) |
| title_full_unstemmed | Structural Stabilities and Transformation Mechanism of Rhynchophylline and Isorhynchophylline by Ultra Performance Liquid Chromatography/Time-of-Flight Mass Spectrometry (UPLC/Q-TOF-MS) |
| title_short | Structural Stabilities and Transformation Mechanism of Rhynchophylline and Isorhynchophylline by Ultra Performance Liquid Chromatography/Time-of-Flight Mass Spectrometry (UPLC/Q-TOF-MS) |
| title_sort | structural stabilities and transformation mechanism of rhynchophylline and isorhynchophylline by ultra performance liquid chromatography time of flight mass spectrometry uplc q tof ms |
| topic | rhynchophylline isorhynchophylline conversion rate stability UPLC-Q-TOF-MS |
| url | http://www.mdpi.com/1420-3049/20/8/14849 |
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