Effect of astaxanthin nanoparticles in protecting the post-thawing quality of rooster sperm challenged by cadmium administration
The protective role of astaxanthin nanoparticles (Ast NPs, 25 mg/kg p.o) against cadmium (Cd, 1 mg/100 g b.w. SC), a known inductor of lipid peroxidation and changes in the antioxidant defense system in the Ross 308 breeder roosters sperm, was examined. Sperm motility (computer-assisted sperm motili...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-03-01
|
| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579119579172 |
| _version_ | 1830477204092354560 |
|---|---|
| author | Davood Najafi Ramezan Ali Taheri Abouzar Najafi Mohammad Shamsollahi Manuel Alvarez-Rodriguez |
| author_facet | Davood Najafi Ramezan Ali Taheri Abouzar Najafi Mohammad Shamsollahi Manuel Alvarez-Rodriguez |
| author_sort | Davood Najafi |
| collection | DOAJ |
| description | The protective role of astaxanthin nanoparticles (Ast NPs, 25 mg/kg p.o) against cadmium (Cd, 1 mg/100 g b.w. SC), a known inductor of lipid peroxidation and changes in the antioxidant defense system in the Ross 308 breeder roosters sperm, was examined. Sperm motility (computer-assisted sperm motility analysis), membrane integrity (hypoosmotic swelling test), viability, total abnormality, and enzymatic parameters were assessed after thawing. The testis/body weight (mg/kg) ratio and HE staining results of testis were also performed. The obtained results showed that Cd induced detrimental effects on testis and sperm, while Cd treated by Ast NPs (Cd Ast) diminished this change compared to the Cd group. Cd-treated group resulted in significantly (P < 0.05) lowest total (37.29 ± 2.46) and progressive (5.84 ± 0.47) motility and decreased antioxidant enzyme activity (CAT, TAC, and GPx), as well as producing a significant (P < 0.05) decrease in testis weight (mg) compared to the control group. Treatment with Ast NPs (Ast NPs + Cd) had reversed Cd-induced changes in the antioxidant defense system and significantly prevented Cd-induced testis damage. In conclusion, the results of our work suggest that Ast NPs at 25 mg/kg act as a potent antioxidant in protecting rooster testes against oxidative stress induced by Cd. |
| first_indexed | 2024-12-21T16:09:12Z |
| format | Article |
| id | doaj.art-b3f23a758ac141e29924062b9551d4bd |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-12-21T16:09:12Z |
| publishDate | 2020-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-b3f23a758ac141e29924062b9551d4bd2022-12-21T18:57:50ZengElsevierPoultry Science0032-57912020-03-0199316781686Effect of astaxanthin nanoparticles in protecting the post-thawing quality of rooster sperm challenged by cadmium administrationDavood Najafi0Ramezan Ali Taheri1Abouzar Najafi2Mohammad Shamsollahi3Manuel Alvarez-Rodriguez4Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, 17166 Tehran, IranNanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, 17166 Tehran, Iran; Corresponding author:Department of Animal Science, College of Agriculture, University of Tabriz, 51666 Tabriz, IranDepartment of Animal Science, College of Agriculture, University of Tabriz, 51666 Tabriz, IranDepartment of Clinical and Experimental Medicine (IKE), Division of Clinical Sciences, Obstetrics and Gynecology, Linköping University, 58113 Linköping, Sweden; Department of Animal Health and Anatomy, Veterinary Faculty, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, SpainThe protective role of astaxanthin nanoparticles (Ast NPs, 25 mg/kg p.o) against cadmium (Cd, 1 mg/100 g b.w. SC), a known inductor of lipid peroxidation and changes in the antioxidant defense system in the Ross 308 breeder roosters sperm, was examined. Sperm motility (computer-assisted sperm motility analysis), membrane integrity (hypoosmotic swelling test), viability, total abnormality, and enzymatic parameters were assessed after thawing. The testis/body weight (mg/kg) ratio and HE staining results of testis were also performed. The obtained results showed that Cd induced detrimental effects on testis and sperm, while Cd treated by Ast NPs (Cd Ast) diminished this change compared to the Cd group. Cd-treated group resulted in significantly (P < 0.05) lowest total (37.29 ± 2.46) and progressive (5.84 ± 0.47) motility and decreased antioxidant enzyme activity (CAT, TAC, and GPx), as well as producing a significant (P < 0.05) decrease in testis weight (mg) compared to the control group. Treatment with Ast NPs (Ast NPs + Cd) had reversed Cd-induced changes in the antioxidant defense system and significantly prevented Cd-induced testis damage. In conclusion, the results of our work suggest that Ast NPs at 25 mg/kg act as a potent antioxidant in protecting rooster testes against oxidative stress induced by Cd.http://www.sciencedirect.com/science/article/pii/S0032579119579172astaxanthinnanoparticlecadmiumroostertoxicity |
| spellingShingle | Davood Najafi Ramezan Ali Taheri Abouzar Najafi Mohammad Shamsollahi Manuel Alvarez-Rodriguez Effect of astaxanthin nanoparticles in protecting the post-thawing quality of rooster sperm challenged by cadmium administration Poultry Science astaxanthin nanoparticle cadmium rooster toxicity |
| title | Effect of astaxanthin nanoparticles in protecting the post-thawing quality of rooster sperm challenged by cadmium administration |
| title_full | Effect of astaxanthin nanoparticles in protecting the post-thawing quality of rooster sperm challenged by cadmium administration |
| title_fullStr | Effect of astaxanthin nanoparticles in protecting the post-thawing quality of rooster sperm challenged by cadmium administration |
| title_full_unstemmed | Effect of astaxanthin nanoparticles in protecting the post-thawing quality of rooster sperm challenged by cadmium administration |
| title_short | Effect of astaxanthin nanoparticles in protecting the post-thawing quality of rooster sperm challenged by cadmium administration |
| title_sort | effect of astaxanthin nanoparticles in protecting the post thawing quality of rooster sperm challenged by cadmium administration |
| topic | astaxanthin nanoparticle cadmium rooster toxicity |
| url | http://www.sciencedirect.com/science/article/pii/S0032579119579172 |
| work_keys_str_mv | AT davoodnajafi effectofastaxanthinnanoparticlesinprotectingthepostthawingqualityofroosterspermchallengedbycadmiumadministration AT ramezanalitaheri effectofastaxanthinnanoparticlesinprotectingthepostthawingqualityofroosterspermchallengedbycadmiumadministration AT abouzarnajafi effectofastaxanthinnanoparticlesinprotectingthepostthawingqualityofroosterspermchallengedbycadmiumadministration AT mohammadshamsollahi effectofastaxanthinnanoparticlesinprotectingthepostthawingqualityofroosterspermchallengedbycadmiumadministration AT manuelalvarezrodriguez effectofastaxanthinnanoparticlesinprotectingthepostthawingqualityofroosterspermchallengedbycadmiumadministration |