Preparation of ZIF-8 and Its Application in Determination of Pyridoxine and Pyridoxal in Ginkgo Seeds by Ultra-Performance Liquid Chromatography
A new rapid and accurate method was developed for simultaneous determination of pyridoxine and pyridoxal in ginkgo seeds, using ultra-performance liquid chromatography (UPLC) equipped with a fluorescence detector. Diluted hydrochloric acid solution was used as the extracting solvent. For the pretrea...
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MDPI AG
2022-07-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/14/2014 |
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| author | Yuan Gao Mengjia Xu Zhe Zheng Yiqun Wan Shihang Wu Chang Li |
| author_facet | Yuan Gao Mengjia Xu Zhe Zheng Yiqun Wan Shihang Wu Chang Li |
| author_sort | Yuan Gao |
| collection | DOAJ |
| description | A new rapid and accurate method was developed for simultaneous determination of pyridoxine and pyridoxal in ginkgo seeds, using ultra-performance liquid chromatography (UPLC) equipped with a fluorescence detector. Diluted hydrochloric acid solution was used as the extracting solvent. For the pretreatment of extracts, a zeolitic imidazolate framework material (ZIF-8) was prepared and characterized. An ODS-BP column (4.6 mm × 250 mm × 5 μm) was used for separation. The conditions of sample extraction, cleaning and separation were optimized. The linear correlation coefficient (R<sup>2</sup>) of the analyte was better than 0.9999, indicating good linearity. The limits of detection (LODs) of pyridoxal and pyridoxine were 0.0065 mg/kg and 0.0057 mg/kg, respectively, and limits of quantitation (LOQs) were 0.022 mg/kg and 0.019 mg/kg, respectively. The recovery of the two substances ranged from 86.2% to 110.4%, and the relative standard deviation (n = 6) was less than 7.5%. The method was applied to determine the contents of pyridoxine and pyridoxal in actual ginkgo seed samples with satisfactory results. |
| first_indexed | 2024-03-09T10:19:32Z |
| format | Article |
| id | doaj.art-b3f5836d959f4356a7edb1cedaffd47e |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T10:19:32Z |
| publishDate | 2022-07-01 |
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| series | Foods |
| spelling | doaj.art-b3f5836d959f4356a7edb1cedaffd47e2023-12-01T22:08:45ZengMDPI AGFoods2304-81582022-07-011114201410.3390/foods11142014Preparation of ZIF-8 and Its Application in Determination of Pyridoxine and Pyridoxal in Ginkgo Seeds by Ultra-Performance Liquid ChromatographyYuan Gao0Mengjia Xu1Zhe Zheng2Yiqun Wan3Shihang Wu4Chang Li5College of Chemistry, Nanchang University, Nanchang 330047, ChinaCollege of Chemistry, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, China-Canada Joint Laboratory of Food Science and Technology (Nanchang), Key Laboratory of Bioactive Polysaccharides of Jiangxi Province, Nanchang University, Nanchang 330047, ChinaCollege of Chemistry, Nanchang University, Nanchang 330047, ChinaAffiliated School, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, China-Canada Joint Laboratory of Food Science and Technology (Nanchang), Key Laboratory of Bioactive Polysaccharides of Jiangxi Province, Nanchang University, Nanchang 330047, ChinaA new rapid and accurate method was developed for simultaneous determination of pyridoxine and pyridoxal in ginkgo seeds, using ultra-performance liquid chromatography (UPLC) equipped with a fluorescence detector. Diluted hydrochloric acid solution was used as the extracting solvent. For the pretreatment of extracts, a zeolitic imidazolate framework material (ZIF-8) was prepared and characterized. An ODS-BP column (4.6 mm × 250 mm × 5 μm) was used for separation. The conditions of sample extraction, cleaning and separation were optimized. The linear correlation coefficient (R<sup>2</sup>) of the analyte was better than 0.9999, indicating good linearity. The limits of detection (LODs) of pyridoxal and pyridoxine were 0.0065 mg/kg and 0.0057 mg/kg, respectively, and limits of quantitation (LOQs) were 0.022 mg/kg and 0.019 mg/kg, respectively. The recovery of the two substances ranged from 86.2% to 110.4%, and the relative standard deviation (n = 6) was less than 7.5%. The method was applied to determine the contents of pyridoxine and pyridoxal in actual ginkgo seed samples with satisfactory results.https://www.mdpi.com/2304-8158/11/14/2014ginkgo seedspyridoxinepyridoxalZIF-8UPLC |
| spellingShingle | Yuan Gao Mengjia Xu Zhe Zheng Yiqun Wan Shihang Wu Chang Li Preparation of ZIF-8 and Its Application in Determination of Pyridoxine and Pyridoxal in Ginkgo Seeds by Ultra-Performance Liquid Chromatography Foods ginkgo seeds pyridoxine pyridoxal ZIF-8 UPLC |
| title | Preparation of ZIF-8 and Its Application in Determination of Pyridoxine and Pyridoxal in Ginkgo Seeds by Ultra-Performance Liquid Chromatography |
| title_full | Preparation of ZIF-8 and Its Application in Determination of Pyridoxine and Pyridoxal in Ginkgo Seeds by Ultra-Performance Liquid Chromatography |
| title_fullStr | Preparation of ZIF-8 and Its Application in Determination of Pyridoxine and Pyridoxal in Ginkgo Seeds by Ultra-Performance Liquid Chromatography |
| title_full_unstemmed | Preparation of ZIF-8 and Its Application in Determination of Pyridoxine and Pyridoxal in Ginkgo Seeds by Ultra-Performance Liquid Chromatography |
| title_short | Preparation of ZIF-8 and Its Application in Determination of Pyridoxine and Pyridoxal in Ginkgo Seeds by Ultra-Performance Liquid Chromatography |
| title_sort | preparation of zif 8 and its application in determination of pyridoxine and pyridoxal in ginkgo seeds by ultra performance liquid chromatography |
| topic | ginkgo seeds pyridoxine pyridoxal ZIF-8 UPLC |
| url | https://www.mdpi.com/2304-8158/11/14/2014 |
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