<i>Brevundimonas vesicularis</i> (S1T13) Mitigates Drought-Stress-Associated Damage in <i>Arabidopsis thaliana</i>
Drought stress is a significant threat to agricultural productivity and poses challenges to plant survival and growth. Research into microbial plant biostimulants faces difficulties in understanding complicated ecological dynamics, molecular mechanisms, and specificity; to address these knowledge ga...
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MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/23/16590 |
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| author | Can Thi My Tran Tiba Nazar Ibrahim Al Azzawi Murtaza Khan Sajid Ali Yong-Sun Moon Byung-Wook Yun |
| author_facet | Can Thi My Tran Tiba Nazar Ibrahim Al Azzawi Murtaza Khan Sajid Ali Yong-Sun Moon Byung-Wook Yun |
| author_sort | Can Thi My Tran |
| collection | DOAJ |
| description | Drought stress is a significant threat to agricultural productivity and poses challenges to plant survival and growth. Research into microbial plant biostimulants faces difficulties in understanding complicated ecological dynamics, molecular mechanisms, and specificity; to address these knowledge gaps, collaborative efforts and innovative strategies are needed. In the present study, we investigated the potential role of <i>Brevundimonas vesicularis</i> (S1T13) as a microbial plant biostimulant to enhance drought tolerance in <i>Arabidopsis thaliana</i>. We assessed the impact of S1T13 on Col-0 wild-type (WT) and <i>atnced3</i> mutant plants under drought conditions. Our results revealed that the inoculation of S1T13 significantly contributed to plant vigor, with notable improvements observed in both genotypes. To elucidate the underlying mechanisms, we studied the role of ROS and their regulation by antioxidant genes and enzymes in plants inoculated with S1T13. Interestingly, the inoculation of S1T13 enhanced the activities of GSH, SOD, POD, and PPO by 33, 35, 41, and 44% in WT and 24, 22, 26, and 33% in <i>atnced3</i>, respectively. In addition, S1T13 upregulated the expression of antioxidant genes. This enhanced antioxidant machinery played a crucial role in neutralizing ROS and protecting plant cells from oxidative damage during drought stress. Furthermore, we investigated the impact of S1T13 on ABA and drought-stress-responsive genes. Similarly, S1T13 modulated the production of ABA and expression of <i>AO3</i>, <i>ABA3</i>, <i>DREB1A</i>, and <i>DREB2A</i> by 31, 42, 37, 41, and 42% in WT and 20, 29, 27, 38, and 29% in <i>atnced3</i>. The improvement in plant vigor, coupled with the induction of the antioxidant system and modulation of ABA, indicates the pivotal role of S1T13 in enhancing the drought stress tolerance of the plants. Conclusively, the current study provides valuable insights for the application of multitrait S1T13 as a novel strain to improve drought stress tolerance in plants and could be added to the consortium of biofertilizers. |
| first_indexed | 2024-03-09T01:51:07Z |
| format | Article |
| id | doaj.art-b3f5d3bb58ec40dfbec1b4aef66c50f8 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T01:51:07Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b3f5d3bb58ec40dfbec1b4aef66c50f82023-12-08T15:16:27ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124231659010.3390/ijms242316590<i>Brevundimonas vesicularis</i> (S1T13) Mitigates Drought-Stress-Associated Damage in <i>Arabidopsis thaliana</i>Can Thi My Tran0Tiba Nazar Ibrahim Al Azzawi1Murtaza Khan2Sajid Ali3Yong-Sun Moon4Byung-Wook Yun5Department of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Horticulture and Life Science, Yeungnam University, Gyeongsan 38541, Republic of KoreaDepartment of Horticulture and Life Science, Yeungnam University, Gyeongsan 38541, Republic of KoreaDepartment of Horticulture and Life Science, Yeungnam University, Gyeongsan 38541, Republic of KoreaDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaDrought stress is a significant threat to agricultural productivity and poses challenges to plant survival and growth. Research into microbial plant biostimulants faces difficulties in understanding complicated ecological dynamics, molecular mechanisms, and specificity; to address these knowledge gaps, collaborative efforts and innovative strategies are needed. In the present study, we investigated the potential role of <i>Brevundimonas vesicularis</i> (S1T13) as a microbial plant biostimulant to enhance drought tolerance in <i>Arabidopsis thaliana</i>. We assessed the impact of S1T13 on Col-0 wild-type (WT) and <i>atnced3</i> mutant plants under drought conditions. Our results revealed that the inoculation of S1T13 significantly contributed to plant vigor, with notable improvements observed in both genotypes. To elucidate the underlying mechanisms, we studied the role of ROS and their regulation by antioxidant genes and enzymes in plants inoculated with S1T13. Interestingly, the inoculation of S1T13 enhanced the activities of GSH, SOD, POD, and PPO by 33, 35, 41, and 44% in WT and 24, 22, 26, and 33% in <i>atnced3</i>, respectively. In addition, S1T13 upregulated the expression of antioxidant genes. This enhanced antioxidant machinery played a crucial role in neutralizing ROS and protecting plant cells from oxidative damage during drought stress. Furthermore, we investigated the impact of S1T13 on ABA and drought-stress-responsive genes. Similarly, S1T13 modulated the production of ABA and expression of <i>AO3</i>, <i>ABA3</i>, <i>DREB1A</i>, and <i>DREB2A</i> by 31, 42, 37, 41, and 42% in WT and 20, 29, 27, 38, and 29% in <i>atnced3</i>. The improvement in plant vigor, coupled with the induction of the antioxidant system and modulation of ABA, indicates the pivotal role of S1T13 in enhancing the drought stress tolerance of the plants. Conclusively, the current study provides valuable insights for the application of multitrait S1T13 as a novel strain to improve drought stress tolerance in plants and could be added to the consortium of biofertilizers.https://www.mdpi.com/1422-0067/24/23/16590drought stressPGPRBantioxidantsgene expressionABA<i>Arabidopsis</i> |
| spellingShingle | Can Thi My Tran Tiba Nazar Ibrahim Al Azzawi Murtaza Khan Sajid Ali Yong-Sun Moon Byung-Wook Yun <i>Brevundimonas vesicularis</i> (S1T13) Mitigates Drought-Stress-Associated Damage in <i>Arabidopsis thaliana</i> International Journal of Molecular Sciences drought stress PGPRB antioxidants gene expression ABA <i>Arabidopsis</i> |
| title | <i>Brevundimonas vesicularis</i> (S1T13) Mitigates Drought-Stress-Associated Damage in <i>Arabidopsis thaliana</i> |
| title_full | <i>Brevundimonas vesicularis</i> (S1T13) Mitigates Drought-Stress-Associated Damage in <i>Arabidopsis thaliana</i> |
| title_fullStr | <i>Brevundimonas vesicularis</i> (S1T13) Mitigates Drought-Stress-Associated Damage in <i>Arabidopsis thaliana</i> |
| title_full_unstemmed | <i>Brevundimonas vesicularis</i> (S1T13) Mitigates Drought-Stress-Associated Damage in <i>Arabidopsis thaliana</i> |
| title_short | <i>Brevundimonas vesicularis</i> (S1T13) Mitigates Drought-Stress-Associated Damage in <i>Arabidopsis thaliana</i> |
| title_sort | i brevundimonas vesicularis i s1t13 mitigates drought stress associated damage in i arabidopsis thaliana i |
| topic | drought stress PGPRB antioxidants gene expression ABA <i>Arabidopsis</i> |
| url | https://www.mdpi.com/1422-0067/24/23/16590 |
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