Functional characterization of ZjPYL8 from sour jujube: enhancing the sensitivity of stomata and roots to ABA in Arabidopsis thaliana
Abscisic acid (ABA) is a plant hormone that plays a crucial role in regulating plant growth, development, and adaptation to stress. The growth of Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H. Chou, commonly known as Suanzao in Chinese, is significantly influenced by environmental factors, par...
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| Format: | Article |
| Language: | English |
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University of Life Sciences in Lublin - Publishing House
2023-12-01
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| Series: | Acta Scientiarum Polonorum: Hortorum Cultus |
| Subjects: | |
| Online Access: | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/5154 |
| _version_ | 1827387223129980928 |
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| author | Peiyan Wang Lanting Qi Junna Song Ruojia Zhu Xiaowei Han Yu Liu Xianyun Wang Yuguang Zheng Zhao Liu |
| author_facet | Peiyan Wang Lanting Qi Junna Song Ruojia Zhu Xiaowei Han Yu Liu Xianyun Wang Yuguang Zheng Zhao Liu |
| author_sort | Peiyan Wang |
| collection | DOAJ |
| description |
Abscisic acid (ABA) is a plant hormone that plays a crucial role in regulating plant growth, development, and adaptation to stress. The growth of Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H. Chou, commonly known as Suanzao in Chinese, is significantly influenced by environmental factors, particularly drought and salt stresses. In this study, we isolated and characterized a putative ABA receptor, ZjPYL8, from Sour jujube. To investigate the effects of ZjPYL8 overexpression on ABA-responsive pathways, we introduced it into Arabidopsis thaliana (A. thaliana) and examined the resulting phenotypes. Our results demonstrated that overexpression of ZjPYL8 in A. thaliana led to a significant reduction in stomatal aperture and root length under ABA treatment, while the wild type (WT) was relatively insensitive to ABA. Moreover, ZjPYL8 transgenic plants exhibited shorter roots under salt treatment than the WT did. These findings suggest that the overexpression of ZjPYL8 in A. thaliana enhances the plant's resistance to stress and support the hypothesis that ZjPYL8 serves as a putative ABA receptor in Sour jujube, which may improve the plant's adaptability to drought and salt stresses. ZjPYL8 appears to mediate plant responses to ABA, similar to most ABA receptors in A. thaliana, such as stomatal closure and root length.
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| first_indexed | 2024-03-08T15:58:48Z |
| format | Article |
| id | doaj.art-b3f6ff6d2ec44c819f87552649d1961d |
| institution | Directory Open Access Journal |
| issn | 1644-0692 2545-1405 |
| language | English |
| last_indexed | 2024-03-08T15:58:48Z |
| publishDate | 2023-12-01 |
| publisher | University of Life Sciences in Lublin - Publishing House |
| record_format | Article |
| series | Acta Scientiarum Polonorum: Hortorum Cultus |
| spelling | doaj.art-b3f6ff6d2ec44c819f87552649d1961d2024-01-08T13:00:11ZengUniversity of Life Sciences in Lublin - Publishing HouseActa Scientiarum Polonorum: Hortorum Cultus1644-06922545-14052023-12-0122610.24326/asp.hc.2023.5154Functional characterization of ZjPYL8 from sour jujube: enhancing the sensitivity of stomata and roots to ABA in Arabidopsis thalianaPeiyan Wang0Lanting Qi1Junna Song2Ruojia Zhu3Xiaowei Han4Yu Liu5Xianyun Wang6Yuguang Zheng7Zhao Liu81 Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, College of Pharmacy, Shijiazhuang, Hebei 050200, China; 2 College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, China College of Life and Environment Science, Minzu University of China, Beijing 100081, China1 Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, College of Pharmacy, Shijiazhuang, Hebei 050200, China; 2 College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, China 1 Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, College of Pharmacy, Shijiazhuang, Hebei 050200, China; 2 College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, China 1 Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, College of Pharmacy, Shijiazhuang, Hebei 050200, China; 2 College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, ChinaCollege of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, ChinaCell Therapy Laboratory, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei 050031, China1 Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, College of Pharmacy, Shijiazhuang, Hebei 050200, China; 2 College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, China1 Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, College of Pharmacy, Shijiazhuang, Hebei 050200, China; 2 College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, China Abscisic acid (ABA) is a plant hormone that plays a crucial role in regulating plant growth, development, and adaptation to stress. The growth of Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H. Chou, commonly known as Suanzao in Chinese, is significantly influenced by environmental factors, particularly drought and salt stresses. In this study, we isolated and characterized a putative ABA receptor, ZjPYL8, from Sour jujube. To investigate the effects of ZjPYL8 overexpression on ABA-responsive pathways, we introduced it into Arabidopsis thaliana (A. thaliana) and examined the resulting phenotypes. Our results demonstrated that overexpression of ZjPYL8 in A. thaliana led to a significant reduction in stomatal aperture and root length under ABA treatment, while the wild type (WT) was relatively insensitive to ABA. Moreover, ZjPYL8 transgenic plants exhibited shorter roots under salt treatment than the WT did. These findings suggest that the overexpression of ZjPYL8 in A. thaliana enhances the plant's resistance to stress and support the hypothesis that ZjPYL8 serves as a putative ABA receptor in Sour jujube, which may improve the plant's adaptability to drought and salt stresses. ZjPYL8 appears to mediate plant responses to ABA, similar to most ABA receptors in A. thaliana, such as stomatal closure and root length. https://czasopisma.up.lublin.pl/index.php/asphc/article/view/5154ZjPYL8osmotic stresssalt stressabscisic acidsour jujube |
| spellingShingle | Peiyan Wang Lanting Qi Junna Song Ruojia Zhu Xiaowei Han Yu Liu Xianyun Wang Yuguang Zheng Zhao Liu Functional characterization of ZjPYL8 from sour jujube: enhancing the sensitivity of stomata and roots to ABA in Arabidopsis thaliana Acta Scientiarum Polonorum: Hortorum Cultus ZjPYL8 osmotic stress salt stress abscisic acid sour jujube |
| title | Functional characterization of ZjPYL8 from sour jujube: enhancing the sensitivity of stomata and roots to ABA in Arabidopsis thaliana |
| title_full | Functional characterization of ZjPYL8 from sour jujube: enhancing the sensitivity of stomata and roots to ABA in Arabidopsis thaliana |
| title_fullStr | Functional characterization of ZjPYL8 from sour jujube: enhancing the sensitivity of stomata and roots to ABA in Arabidopsis thaliana |
| title_full_unstemmed | Functional characterization of ZjPYL8 from sour jujube: enhancing the sensitivity of stomata and roots to ABA in Arabidopsis thaliana |
| title_short | Functional characterization of ZjPYL8 from sour jujube: enhancing the sensitivity of stomata and roots to ABA in Arabidopsis thaliana |
| title_sort | functional characterization of zjpyl8 from sour jujube enhancing the sensitivity of stomata and roots to aba in arabidopsis thaliana |
| topic | ZjPYL8 osmotic stress salt stress abscisic acid sour jujube |
| url | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/5154 |
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