Oat fiber attenuates circulating oxysterols levels and hepatic inflammation via targeting TLR4 signal pathway in LDL receptor knockout mice
Oxidized forms of cholesterol, oxysterols which involved in lipid homeostasis and inflammatory reaction, played a vital role in the formation of atherosclerotic lesion. The present study aimed to explore how oat fiber affected circulating oxysterols and targeted hepatic inflammation in LDLR−/− mice....
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-01-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464620305466 |
| _version_ | 1818563227101954048 |
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| author | Hui Gao Chunping Rao Ruijuan Song Shufen Han |
| author_facet | Hui Gao Chunping Rao Ruijuan Song Shufen Han |
| author_sort | Hui Gao |
| collection | DOAJ |
| description | Oxidized forms of cholesterol, oxysterols which involved in lipid homeostasis and inflammatory reaction, played a vital role in the formation of atherosclerotic lesion. The present study aimed to explore how oat fiber affected circulating oxysterols and targeted hepatic inflammation in LDLR−/− mice. Animals were given a high-fat/cholesterol (HFC) diet without or with 0.8% oat fiber for 14 weeks. Oat fiber decreased serum LDL-c level, accompanying by a reduction of hepatic lipid accumulation and atherosclerotic lesion. The increment of circulating oxysterols, including 7-HC, 25-HC, 27-HC and 6a-Hydroxy-5a-cholestanol caused by a HFC diet in LDLR−/− mice, were inhibited by oat fiber feeding. Notably, oat fiber blocked TLR4 signal pathway via downregulating the downstream adaptor MyD88 and activating NF-kB p65 signal. In summary, the present study provided experimental evidence that oat fiber feeding retarded the progression of atherosclerosis via acting on circulating oxysterols and ameliorating hepatic inflammatory reaction by targeting TLR4/MyD88/NF-κB signal pathway. |
| first_indexed | 2024-12-14T01:13:59Z |
| format | Article |
| id | doaj.art-b3f795b18e044095a4833b942db3a9f9 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-14T01:13:59Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-b3f795b18e044095a4833b942db3a9f92022-12-21T23:22:39ZengElsevierJournal of Functional Foods1756-46462021-01-0176104322Oat fiber attenuates circulating oxysterols levels and hepatic inflammation via targeting TLR4 signal pathway in LDL receptor knockout miceHui Gao0Chunping Rao1Ruijuan Song2Shufen Han3Department of Nutrition and Food Hygiene, Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Disease, School of Public Health, Soochow University, Suzhou, ChinaSuzhou Vocational Health College, Suzhou, ChinaDepartment of Nutrition and Food Hygiene, Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Disease, School of Public Health, Soochow University, Suzhou, ChinaDepartment of Nutrition and Food Hygiene, Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Disease, School of Public Health, Soochow University, Suzhou, China; Corresponding author at: Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, 199 Renai Road, Suzhou 215123, China.Oxidized forms of cholesterol, oxysterols which involved in lipid homeostasis and inflammatory reaction, played a vital role in the formation of atherosclerotic lesion. The present study aimed to explore how oat fiber affected circulating oxysterols and targeted hepatic inflammation in LDLR−/− mice. Animals were given a high-fat/cholesterol (HFC) diet without or with 0.8% oat fiber for 14 weeks. Oat fiber decreased serum LDL-c level, accompanying by a reduction of hepatic lipid accumulation and atherosclerotic lesion. The increment of circulating oxysterols, including 7-HC, 25-HC, 27-HC and 6a-Hydroxy-5a-cholestanol caused by a HFC diet in LDLR−/− mice, were inhibited by oat fiber feeding. Notably, oat fiber blocked TLR4 signal pathway via downregulating the downstream adaptor MyD88 and activating NF-kB p65 signal. In summary, the present study provided experimental evidence that oat fiber feeding retarded the progression of atherosclerosis via acting on circulating oxysterols and ameliorating hepatic inflammatory reaction by targeting TLR4/MyD88/NF-κB signal pathway.http://www.sciencedirect.com/science/article/pii/S1756464620305466Oat fiberOxysterolsTLR4/MyD88/NF-κB pathwayLipid metabolismAtherosclerosis |
| spellingShingle | Hui Gao Chunping Rao Ruijuan Song Shufen Han Oat fiber attenuates circulating oxysterols levels and hepatic inflammation via targeting TLR4 signal pathway in LDL receptor knockout mice Journal of Functional Foods Oat fiber Oxysterols TLR4/MyD88/NF-κB pathway Lipid metabolism Atherosclerosis |
| title | Oat fiber attenuates circulating oxysterols levels and hepatic inflammation via targeting TLR4 signal pathway in LDL receptor knockout mice |
| title_full | Oat fiber attenuates circulating oxysterols levels and hepatic inflammation via targeting TLR4 signal pathway in LDL receptor knockout mice |
| title_fullStr | Oat fiber attenuates circulating oxysterols levels and hepatic inflammation via targeting TLR4 signal pathway in LDL receptor knockout mice |
| title_full_unstemmed | Oat fiber attenuates circulating oxysterols levels and hepatic inflammation via targeting TLR4 signal pathway in LDL receptor knockout mice |
| title_short | Oat fiber attenuates circulating oxysterols levels and hepatic inflammation via targeting TLR4 signal pathway in LDL receptor knockout mice |
| title_sort | oat fiber attenuates circulating oxysterols levels and hepatic inflammation via targeting tlr4 signal pathway in ldl receptor knockout mice |
| topic | Oat fiber Oxysterols TLR4/MyD88/NF-κB pathway Lipid metabolism Atherosclerosis |
| url | http://www.sciencedirect.com/science/article/pii/S1756464620305466 |
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