Myogenesis Effects of RGX365 to Improve Skeletal Muscle Atrophy
Age-related skeletal muscle atrophy and weakness not only reduce the quality of life of those afflicted, but also worsen the prognosis of underlying diseases. We evaluated the effect of RGX365, a protopanaxatriol-type rare ginsenoside mixture, on improving skeletal muscle atrophy. We investigated th...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/15/19/4307 |
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| author | Hye-Jin Lee Hui-Ji Choi Sang-Ah Lee Dong Hyuk Baek Jong Beom Heo Gyu Yong Song Wonhwa Lee |
| author_facet | Hye-Jin Lee Hui-Ji Choi Sang-Ah Lee Dong Hyuk Baek Jong Beom Heo Gyu Yong Song Wonhwa Lee |
| author_sort | Hye-Jin Lee |
| collection | DOAJ |
| description | Age-related skeletal muscle atrophy and weakness not only reduce the quality of life of those afflicted, but also worsen the prognosis of underlying diseases. We evaluated the effect of RGX365, a protopanaxatriol-type rare ginsenoside mixture, on improving skeletal muscle atrophy. We investigated the myogenic effect of RGX365 on mouse myoblast cells (C2C12) and dexamethasone (10 µM)-induced atrophy of differentiated C2C12. RGX365-treated myotube diameters and myosin heavy chain (MyHC) expression levels were analyzed using immunofluorescence. We evaluated the myogenic effects of RGX365 in aging sarcopenic mice. RGX365 increased myoblast differentiation and MyHC expression, and attenuated the muscle atrophy-inducing F-box (Atrogin-1) and muscle RING finger 1 (MuRF1) expression. Notably, one month of oral administration of RGX365 to 23-month-old sarcopenic mice improved muscle fiber size and the expression of skeletal muscle regeneration-associated molecules. In conclusion, rare ginsenosides, agonists of steroid receptors, can ameliorate skeletal muscle atrophy during long-term administration. |
| first_indexed | 2024-03-10T21:38:01Z |
| format | Article |
| id | doaj.art-b3f9abf658c341d9a91be944b90fcacd |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T21:38:01Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-b3f9abf658c341d9a91be944b90fcacd2023-11-19T14:53:04ZengMDPI AGNutrients2072-66432023-10-011519430710.3390/nu15194307Myogenesis Effects of RGX365 to Improve Skeletal Muscle AtrophyHye-Jin Lee0Hui-Ji Choi1Sang-Ah Lee2Dong Hyuk Baek3Jong Beom Heo4Gyu Yong Song5Wonhwa Lee6Department of Chemistry, Sungkyunkwan University, Suwon 16419, Republic of KoreaCollege of Pharmacy, Chungnam National University, Daejeon 34134, Republic of KoreaFaculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju 63243, Republic of KoreaCollege of Pharmacy, Chungnam National University, Daejeon 34134, Republic of KoreaCollege of Pharmacy, Chungnam National University, Daejeon 34134, Republic of KoreaCollege of Pharmacy, Chungnam National University, Daejeon 34134, Republic of KoreaDepartment of Chemistry, Sungkyunkwan University, Suwon 16419, Republic of KoreaAge-related skeletal muscle atrophy and weakness not only reduce the quality of life of those afflicted, but also worsen the prognosis of underlying diseases. We evaluated the effect of RGX365, a protopanaxatriol-type rare ginsenoside mixture, on improving skeletal muscle atrophy. We investigated the myogenic effect of RGX365 on mouse myoblast cells (C2C12) and dexamethasone (10 µM)-induced atrophy of differentiated C2C12. RGX365-treated myotube diameters and myosin heavy chain (MyHC) expression levels were analyzed using immunofluorescence. We evaluated the myogenic effects of RGX365 in aging sarcopenic mice. RGX365 increased myoblast differentiation and MyHC expression, and attenuated the muscle atrophy-inducing F-box (Atrogin-1) and muscle RING finger 1 (MuRF1) expression. Notably, one month of oral administration of RGX365 to 23-month-old sarcopenic mice improved muscle fiber size and the expression of skeletal muscle regeneration-associated molecules. In conclusion, rare ginsenosides, agonists of steroid receptors, can ameliorate skeletal muscle atrophy during long-term administration.https://www.mdpi.com/2072-6643/15/19/4307RGX365rare ginsenosidessarcopeniamyosin heavy chainmyogenesis |
| spellingShingle | Hye-Jin Lee Hui-Ji Choi Sang-Ah Lee Dong Hyuk Baek Jong Beom Heo Gyu Yong Song Wonhwa Lee Myogenesis Effects of RGX365 to Improve Skeletal Muscle Atrophy Nutrients RGX365 rare ginsenosides sarcopenia myosin heavy chain myogenesis |
| title | Myogenesis Effects of RGX365 to Improve Skeletal Muscle Atrophy |
| title_full | Myogenesis Effects of RGX365 to Improve Skeletal Muscle Atrophy |
| title_fullStr | Myogenesis Effects of RGX365 to Improve Skeletal Muscle Atrophy |
| title_full_unstemmed | Myogenesis Effects of RGX365 to Improve Skeletal Muscle Atrophy |
| title_short | Myogenesis Effects of RGX365 to Improve Skeletal Muscle Atrophy |
| title_sort | myogenesis effects of rgx365 to improve skeletal muscle atrophy |
| topic | RGX365 rare ginsenosides sarcopenia myosin heavy chain myogenesis |
| url | https://www.mdpi.com/2072-6643/15/19/4307 |
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