Polymorphisms of toll-like receptor 2 and 4 genes in Chagas disease
The aim of this study was to test the possible implication of toll-like receptor 2 (TLR2) and TLR4 gene polymorphisms in determining the susceptibility to Chagas' disease. Genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism in 475 individuals from Col...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
2008-02-01
|
| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000100004 |
| _version_ | 1827831901958701056 |
|---|---|
| author | German Zafra Oscar Flórez Carlos A. Morillo Luis Eduardo Echeverría Javier Martín Clara Isabel González |
| author_facet | German Zafra Oscar Flórez Carlos A. Morillo Luis Eduardo Echeverría Javier Martín Clara Isabel González |
| author_sort | German Zafra |
| collection | DOAJ |
| description | The aim of this study was to test the possible implication of toll-like receptor 2 (TLR2) and TLR4 gene polymorphisms in determining the susceptibility to Chagas' disease. Genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism in 475 individuals from Colombia, 143 seropositive with chagasic cardiomyopathy, 132 seropositive asymptomatic and 200 seronegative. The TLR2 arginine to glutamine substitution at residue 753(Arg753Gln) polymorphism was absent in the groups analyzed. The TLR4 Asp299Gly and Thr399Ile polymorphisms are in linkage disequilibrium and we observed a very low frequency of these polymorphisms in our study population (2.6% and 1.8% respectively). The overall TLR2 and TLR4 alleles and genotype distribution in seronegative and seropositive were not significantly different. We compared the frequencies between asymptomatic patients and those with chagasic cardiomyopathy and we did not observe any significant differences in the distribution of alleles or genotypes. In summary, this study corroborates the low frequency of TLR2 and TLR4 polymorphisms observed in other populations and suggest that these do not play an important role in Chagas' disease. The validation of these findings in independent cohorts is needed to firmly establish a role for TLR2 and TLR4 variants in Chagas' disease. |
| first_indexed | 2024-03-12T05:02:03Z |
| format | Article |
| id | doaj.art-b3fa113d0a5b49c281fa6e6460137c04 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T05:02:03Z |
| publishDate | 2008-02-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-b3fa113d0a5b49c281fa6e6460137c042023-09-03T09:11:22ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602008-02-011031273010.1590/S0074-02762008000100004Polymorphisms of toll-like receptor 2 and 4 genes in Chagas diseaseGerman ZafraOscar FlórezCarlos A. MorilloLuis Eduardo EcheverríaJavier MartínClara Isabel GonzálezThe aim of this study was to test the possible implication of toll-like receptor 2 (TLR2) and TLR4 gene polymorphisms in determining the susceptibility to Chagas' disease. Genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism in 475 individuals from Colombia, 143 seropositive with chagasic cardiomyopathy, 132 seropositive asymptomatic and 200 seronegative. The TLR2 arginine to glutamine substitution at residue 753(Arg753Gln) polymorphism was absent in the groups analyzed. The TLR4 Asp299Gly and Thr399Ile polymorphisms are in linkage disequilibrium and we observed a very low frequency of these polymorphisms in our study population (2.6% and 1.8% respectively). The overall TLR2 and TLR4 alleles and genotype distribution in seronegative and seropositive were not significantly different. We compared the frequencies between asymptomatic patients and those with chagasic cardiomyopathy and we did not observe any significant differences in the distribution of alleles or genotypes. In summary, this study corroborates the low frequency of TLR2 and TLR4 polymorphisms observed in other populations and suggest that these do not play an important role in Chagas' disease. The validation of these findings in independent cohorts is needed to firmly establish a role for TLR2 and TLR4 variants in Chagas' disease.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000100004Chagas' diseasepolymorphismtoll-like receptor 2 genetoll-like receptor 4 gene |
| spellingShingle | German Zafra Oscar Flórez Carlos A. Morillo Luis Eduardo Echeverría Javier Martín Clara Isabel González Polymorphisms of toll-like receptor 2 and 4 genes in Chagas disease Memorias do Instituto Oswaldo Cruz Chagas' disease polymorphism toll-like receptor 2 gene toll-like receptor 4 gene |
| title | Polymorphisms of toll-like receptor 2 and 4 genes in Chagas disease |
| title_full | Polymorphisms of toll-like receptor 2 and 4 genes in Chagas disease |
| title_fullStr | Polymorphisms of toll-like receptor 2 and 4 genes in Chagas disease |
| title_full_unstemmed | Polymorphisms of toll-like receptor 2 and 4 genes in Chagas disease |
| title_short | Polymorphisms of toll-like receptor 2 and 4 genes in Chagas disease |
| title_sort | polymorphisms of toll like receptor 2 and 4 genes in chagas disease |
| topic | Chagas' disease polymorphism toll-like receptor 2 gene toll-like receptor 4 gene |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000100004 |
| work_keys_str_mv | AT germanzafra polymorphismsoftolllikereceptor2and4genesinchagasdisease AT oscarflorez polymorphismsoftolllikereceptor2and4genesinchagasdisease AT carlosamorillo polymorphismsoftolllikereceptor2and4genesinchagasdisease AT luiseduardoecheverria polymorphismsoftolllikereceptor2and4genesinchagasdisease AT javiermartin polymorphismsoftolllikereceptor2and4genesinchagasdisease AT claraisabelgonzalez polymorphismsoftolllikereceptor2and4genesinchagasdisease |