Gut fungal community composition analysis of myostatin mutant cattle prepared by CRISPR/Cas9
Myostatin (MSTN) regulates muscle development and body metabolism through a variety of pathways and is a core target gene for gene editing in livestock. Gut fungi constitute a small part of the gut microbiome and are important to host health and metabolism. The influence of MSTN mutations on bovine...
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| Format: | Article |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.1084945/full |
| _version_ | 1797951464134410240 |
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| author | Li Gao Song Wang Song Wang Miaomiao Yang Lili Wang Zhen Li Lei Yang Guangpeng Li Tong Wen |
| author_facet | Li Gao Song Wang Song Wang Miaomiao Yang Lili Wang Zhen Li Lei Yang Guangpeng Li Tong Wen |
| author_sort | Li Gao |
| collection | DOAJ |
| description | Myostatin (MSTN) regulates muscle development and body metabolism through a variety of pathways and is a core target gene for gene editing in livestock. Gut fungi constitute a small part of the gut microbiome and are important to host health and metabolism. The influence of MSTN mutations on bovine gut fungi remains unknown. In this study, Internal Transcribed Spacer (ITS) high-throughput sequencing was conducted to explore the composition of gut fungi in the MSTN mutant (MT) and wild-type (WT) cattle, and 5,861 operational taxonomic units (OTUs) were detected and classified into 16 phyla and 802 genera. The results of the alpha diversity analysis indicated that no notable divergence was displayed between the WT and MT cattle; however, significant differences were noticed in the composition of fungal communities. Eight phyla and 18 genera were detected. According to the prediction of fungal function, saprotroph fungi were significantly more abundant in the MT group. The correlation analysis between gut fungal and bacterial communities revealed that MSTN mutations directly changed the gut fungal composition and, at the same time, influenced some fungi and bacteria by indirectly regulating the interaction between microorganisms, which affected the host metabolism further. This study analyzed the role of MSTN mutations in regulating the host metabolism of intestinal fungi and provided a theoretical basis for the relationship between MSTN and gut fungi. |
| first_indexed | 2024-04-10T22:30:58Z |
| format | Article |
| id | doaj.art-b3fc2bcbf152406fabca9b467e9b60c4 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-10T22:30:58Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-b3fc2bcbf152406fabca9b467e9b60c42023-01-17T04:49:40ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-01-01910.3389/fvets.2022.10849451084945Gut fungal community composition analysis of myostatin mutant cattle prepared by CRISPR/Cas9Li Gao0Song Wang1Song Wang2Miaomiao Yang3Lili Wang4Zhen Li5Lei Yang6Guangpeng Li7Tong Wen8Faculty of Biological Science and Technology, Baotou Teacher's College, Baotou, ChinaCollege of Life Science, Northeast Agricultural University, Harbin, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot, ChinaFaculty of Biological Science and Technology, Baotou Teacher's College, Baotou, ChinaFaculty of Biological Science and Technology, Baotou Teacher's College, Baotou, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot, ChinaFaculty of Biological Science and Technology, Baotou Teacher's College, Baotou, ChinaMyostatin (MSTN) regulates muscle development and body metabolism through a variety of pathways and is a core target gene for gene editing in livestock. Gut fungi constitute a small part of the gut microbiome and are important to host health and metabolism. The influence of MSTN mutations on bovine gut fungi remains unknown. In this study, Internal Transcribed Spacer (ITS) high-throughput sequencing was conducted to explore the composition of gut fungi in the MSTN mutant (MT) and wild-type (WT) cattle, and 5,861 operational taxonomic units (OTUs) were detected and classified into 16 phyla and 802 genera. The results of the alpha diversity analysis indicated that no notable divergence was displayed between the WT and MT cattle; however, significant differences were noticed in the composition of fungal communities. Eight phyla and 18 genera were detected. According to the prediction of fungal function, saprotroph fungi were significantly more abundant in the MT group. The correlation analysis between gut fungal and bacterial communities revealed that MSTN mutations directly changed the gut fungal composition and, at the same time, influenced some fungi and bacteria by indirectly regulating the interaction between microorganisms, which affected the host metabolism further. This study analyzed the role of MSTN mutations in regulating the host metabolism of intestinal fungi and provided a theoretical basis for the relationship between MSTN and gut fungi.https://www.frontiersin.org/articles/10.3389/fvets.2022.1084945/fullMSTN mutantgut fungicattleITShigh-throughput sequencingcorrelation |
| spellingShingle | Li Gao Song Wang Song Wang Miaomiao Yang Lili Wang Zhen Li Lei Yang Guangpeng Li Tong Wen Gut fungal community composition analysis of myostatin mutant cattle prepared by CRISPR/Cas9 Frontiers in Veterinary Science MSTN mutant gut fungi cattle ITS high-throughput sequencing correlation |
| title | Gut fungal community composition analysis of myostatin mutant cattle prepared by CRISPR/Cas9 |
| title_full | Gut fungal community composition analysis of myostatin mutant cattle prepared by CRISPR/Cas9 |
| title_fullStr | Gut fungal community composition analysis of myostatin mutant cattle prepared by CRISPR/Cas9 |
| title_full_unstemmed | Gut fungal community composition analysis of myostatin mutant cattle prepared by CRISPR/Cas9 |
| title_short | Gut fungal community composition analysis of myostatin mutant cattle prepared by CRISPR/Cas9 |
| title_sort | gut fungal community composition analysis of myostatin mutant cattle prepared by crispr cas9 |
| topic | MSTN mutant gut fungi cattle ITS high-throughput sequencing correlation |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.1084945/full |
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