In silico PCR Verification and Simplex Real-Time PCR Detection of Methicillin-Resistant Staphylococcus aureus (MRSA) from East Coast Malaysian Clinical Isolates

In silico PCR Verification and Simplex Real-Time PCR Detection of Methicillin-Resistant Staphylococcus aureus (MRSA) from East Coast Malaysian Clinical Isolates

The aims of this study were to validate the primers developed for molecular-based detection and identification of Malaysian clinical isolates of methicilin-resistance Staphylococcus aureus (MRSA) using in-silico Polymerase Chain Reaction (PCR) and real-time PCR SYBR with Green I. Rapid molecular dia...

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Bibliographic Details
Main Authors: Zarizal Bin SUHAILI, Saiful Azmi JOHARI, Mohammad Hailmi SAJILI, Azifah YAHYA, Zainul Amiruddin ZAKARIA, Mohd Nasir MOHD DESA, Abdul Manaf ALI
Format: Article
Language:English
Published: Walailak University 2013-04-01
Series:Walailak Journal of Science and Technology
Subjects:
In-silico PCR
real-time PCR SYBR Green I
methicillin-resistant Staphylococcus aureus (MRSA)
staphylocoagulase (coa)
nuclease (nuc) and methicillin-resistant (mecA) genes
Online Access:http://wjst.wu.ac.th/index.php/wjst/article/view/262

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