Effects of cadmium exposure on intestinal microflora of Cipangopaludina cathayensis
As one of the most environmentally toxic heavy metals, cadmium (Cd) has attracted the attention of researchers globally. In particular, Guangxi, a province in southwestern China, has been subjected to severe Cd pollution due to geogenic processes and anthropogenic activities. Cd can be accumulated i...
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| Format: | Article |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.984757/full |
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| author | Jiao-yun Jiang Jiao-yun Jiang Jiao-yun Jiang Wen-hong Li Wen-hong Li Yang-yang Wu Chun-xing Cheng Quan-qing Ye Jia-xun Feng Zhi-xun Xie Zhi-xun Xie |
| author_facet | Jiao-yun Jiang Jiao-yun Jiang Jiao-yun Jiang Wen-hong Li Wen-hong Li Yang-yang Wu Chun-xing Cheng Quan-qing Ye Jia-xun Feng Zhi-xun Xie Zhi-xun Xie |
| author_sort | Jiao-yun Jiang |
| collection | DOAJ |
| description | As one of the most environmentally toxic heavy metals, cadmium (Cd) has attracted the attention of researchers globally. In particular, Guangxi, a province in southwestern China, has been subjected to severe Cd pollution due to geogenic processes and anthropogenic activities. Cd can be accumulated in aquatic animals and transferred to the human body through the food chain, with potential health risks. The aim of the present study was to explore the effects of waterborne Cd exposure (0.5 mg/L and 1.5 mg/L) on the intestinal microbiota of mudsnail, Cipangopaludina cathayensis, which is favored by farmers and consumers in Guangxi. Gut bacterial community composition was investigated using high-throughput sequencing of the V3–V4 segment of the bacterial 16S rRNA gene. Our results indicated that C. cathayensis could tolerate low Cd (0.5 mg/L) stress, while Cd exposure at high doses (1.5 mg/L) exerted considerable effects on microbiota composition. At the phylum level, Proteobacteria, Bacteroidetes, and Firmicutes were the dominant phyla in the mudsnail gut microbiota. The relative abundances of Bacteroidetes increased significantly under high Cd exposure (H14) (p < 0.01), with no significant change in the low Cd exposure (L14) treatment. The dominant genera with significant differences in relative abundance were Pseudomonas, Cloacibacterium, Acinetobacter, Dechloromonas, and Rhodobacter. In addition, Cd exposure could significantly alter the pathways associated with metabolism, cellular processes, environmental information processing, genetic information processing, human diseases, and organismal systems. Notably, compared to the L14 treatment, some disease-related pathways were enriched, while some xenobiotic and organic compound biodegradation and metabolism pathways were significantly inhibited in the H14 group. Overall, Cd exposure profoundly influenced community structure and function of gut microbiota, which may in turn influence C. cathayensis gut homeostasis and health. |
| first_indexed | 2024-12-10T17:12:44Z |
| format | Article |
| id | doaj.art-b3fd01a471a24e06995e86ca0d08114c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T17:12:44Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-b3fd01a471a24e06995e86ca0d08114c2022-12-22T01:40:16ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-08-011310.3389/fmicb.2022.984757984757Effects of cadmium exposure on intestinal microflora of Cipangopaludina cathayensisJiao-yun Jiang0Jiao-yun Jiang1Jiao-yun Jiang2Wen-hong Li3Wen-hong Li4Yang-yang Wu5Chun-xing Cheng6Quan-qing Ye7Jia-xun Feng8Zhi-xun Xie9Zhi-xun Xie10College of Life Science and Technology, Guangxi University, Nanning, ChinaGuangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, ChinaKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Normal University, Guilin, ChinaCollege of Life Science and Technology, Guangxi University, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Normal University, Guilin, ChinaKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Normal University, Guilin, ChinaKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Normal University, Guilin, ChinaCollege of Life Science and Technology, Guangxi University, Nanning, ChinaCollege of Life Science and Technology, Guangxi University, Nanning, ChinaGuangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, ChinaAs one of the most environmentally toxic heavy metals, cadmium (Cd) has attracted the attention of researchers globally. In particular, Guangxi, a province in southwestern China, has been subjected to severe Cd pollution due to geogenic processes and anthropogenic activities. Cd can be accumulated in aquatic animals and transferred to the human body through the food chain, with potential health risks. The aim of the present study was to explore the effects of waterborne Cd exposure (0.5 mg/L and 1.5 mg/L) on the intestinal microbiota of mudsnail, Cipangopaludina cathayensis, which is favored by farmers and consumers in Guangxi. Gut bacterial community composition was investigated using high-throughput sequencing of the V3–V4 segment of the bacterial 16S rRNA gene. Our results indicated that C. cathayensis could tolerate low Cd (0.5 mg/L) stress, while Cd exposure at high doses (1.5 mg/L) exerted considerable effects on microbiota composition. At the phylum level, Proteobacteria, Bacteroidetes, and Firmicutes were the dominant phyla in the mudsnail gut microbiota. The relative abundances of Bacteroidetes increased significantly under high Cd exposure (H14) (p < 0.01), with no significant change in the low Cd exposure (L14) treatment. The dominant genera with significant differences in relative abundance were Pseudomonas, Cloacibacterium, Acinetobacter, Dechloromonas, and Rhodobacter. In addition, Cd exposure could significantly alter the pathways associated with metabolism, cellular processes, environmental information processing, genetic information processing, human diseases, and organismal systems. Notably, compared to the L14 treatment, some disease-related pathways were enriched, while some xenobiotic and organic compound biodegradation and metabolism pathways were significantly inhibited in the H14 group. Overall, Cd exposure profoundly influenced community structure and function of gut microbiota, which may in turn influence C. cathayensis gut homeostasis and health.https://www.frontiersin.org/articles/10.3389/fmicb.2022.984757/fullcadmiumCipangopaludina cathayensisintestinal microbiotahigh-throughput sequencingmicrobial diversity |
| spellingShingle | Jiao-yun Jiang Jiao-yun Jiang Jiao-yun Jiang Wen-hong Li Wen-hong Li Yang-yang Wu Chun-xing Cheng Quan-qing Ye Jia-xun Feng Zhi-xun Xie Zhi-xun Xie Effects of cadmium exposure on intestinal microflora of Cipangopaludina cathayensis Frontiers in Microbiology cadmium Cipangopaludina cathayensis intestinal microbiota high-throughput sequencing microbial diversity |
| title | Effects of cadmium exposure on intestinal microflora of Cipangopaludina cathayensis |
| title_full | Effects of cadmium exposure on intestinal microflora of Cipangopaludina cathayensis |
| title_fullStr | Effects of cadmium exposure on intestinal microflora of Cipangopaludina cathayensis |
| title_full_unstemmed | Effects of cadmium exposure on intestinal microflora of Cipangopaludina cathayensis |
| title_short | Effects of cadmium exposure on intestinal microflora of Cipangopaludina cathayensis |
| title_sort | effects of cadmium exposure on intestinal microflora of cipangopaludina cathayensis |
| topic | cadmium Cipangopaludina cathayensis intestinal microbiota high-throughput sequencing microbial diversity |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.984757/full |
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