The Absence of Adiponectin Alters Niacin’s Effects on Adipose Tissue Inflammation in Mice
Obesity is an immunometabolic disease associated with chronic inflammation and the dysregulation of pro- and anti-inflammatory cytokines. One hallmark of obesity is reduced concentrations of the anti-inflammatory adipokine, adiponectin. Pharmacologic doses of niacin produce multiple metabolic benefi...
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MDPI AG
2020-08-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/12/8/2427 |
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| author | Emily C. Graff Han Fang Desiree Wanders Robert L. Judd |
| author_facet | Emily C. Graff Han Fang Desiree Wanders Robert L. Judd |
| author_sort | Emily C. Graff |
| collection | DOAJ |
| description | Obesity is an immunometabolic disease associated with chronic inflammation and the dysregulation of pro- and anti-inflammatory cytokines. One hallmark of obesity is reduced concentrations of the anti-inflammatory adipokine, adiponectin. Pharmacologic doses of niacin produce multiple metabolic benefits, including attenuating high-fat diet (HFD)-induced adipose tissue inflammation and increasing adiponectin concentrations. To determine if adiponectin mediates the anti-inflammatory effects of niacin, male C57BL/6J (WT) and adiponectin null (<i>Adipoq</i><sup>-/-</sup>) mice were maintained on a low-fat diet (LFD) or HFD for 6 weeks, before being administered either vehicle or niacin (360 mg/kg/day) for 5 weeks. HFD-fed mice had increased expression of genes associated with macrophage recruitment (<i>Ccl2</i>) and number (<i>Cd68</i>), and increased crown-like structure (CLS) number in adipose tissue. While niacin attenuated <i>Ccl2</i> expression, there were no effects on <i>Cd68</i> or CLS number. The absence of adiponectin did not hinder the ability of niacin to reduce <i>Ccl2</i> expression. HFD feeding increased gene expression of inflammatory markers in the adipose tissue of WT and <i>Adipoq<sup>-/-</sup></i> mice. While niacin tended to decrease the expression of inflammatory markers in WT mice, niacin increased their expression in HFD-fed <i>Adipoq<sup>-/-</sup></i> mice. Therefore, our results indicate that the absence of adiponectin alters the effects of niacin on markers of adipose tissue inflammation in HFD-fed mice, suggesting that the effects of niacin on tissue cytokines may involve adiponectin. |
| first_indexed | 2024-03-10T17:31:55Z |
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| language | English |
| last_indexed | 2024-03-10T17:31:55Z |
| publishDate | 2020-08-01 |
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| series | Nutrients |
| spelling | doaj.art-b3fd27441ea84e3da8450fb6a452edbf2023-11-20T10:00:06ZengMDPI AGNutrients2072-66432020-08-01128242710.3390/nu12082427The Absence of Adiponectin Alters Niacin’s Effects on Adipose Tissue Inflammation in MiceEmily C. Graff0Han Fang1Desiree Wanders2Robert L. Judd3Department of Pathobiology, Auburn University, Auburn, AL 36849, USAPennington Biomedical Research Center, Louisiana State University, Baton Rouge, LA 70808, USADepartment of Nutrition, Georgia State University, Atlanta, GA 30302, USADepartment of Anatomy, Physiology and Pharmacology, Auburn University, Auburn, AL 36849, USAObesity is an immunometabolic disease associated with chronic inflammation and the dysregulation of pro- and anti-inflammatory cytokines. One hallmark of obesity is reduced concentrations of the anti-inflammatory adipokine, adiponectin. Pharmacologic doses of niacin produce multiple metabolic benefits, including attenuating high-fat diet (HFD)-induced adipose tissue inflammation and increasing adiponectin concentrations. To determine if adiponectin mediates the anti-inflammatory effects of niacin, male C57BL/6J (WT) and adiponectin null (<i>Adipoq</i><sup>-/-</sup>) mice were maintained on a low-fat diet (LFD) or HFD for 6 weeks, before being administered either vehicle or niacin (360 mg/kg/day) for 5 weeks. HFD-fed mice had increased expression of genes associated with macrophage recruitment (<i>Ccl2</i>) and number (<i>Cd68</i>), and increased crown-like structure (CLS) number in adipose tissue. While niacin attenuated <i>Ccl2</i> expression, there were no effects on <i>Cd68</i> or CLS number. The absence of adiponectin did not hinder the ability of niacin to reduce <i>Ccl2</i> expression. HFD feeding increased gene expression of inflammatory markers in the adipose tissue of WT and <i>Adipoq<sup>-/-</sup></i> mice. While niacin tended to decrease the expression of inflammatory markers in WT mice, niacin increased their expression in HFD-fed <i>Adipoq<sup>-/-</sup></i> mice. Therefore, our results indicate that the absence of adiponectin alters the effects of niacin on markers of adipose tissue inflammation in HFD-fed mice, suggesting that the effects of niacin on tissue cytokines may involve adiponectin.https://www.mdpi.com/2072-6643/12/8/2427obesityinflammationimmuneniacinadipokines |
| spellingShingle | Emily C. Graff Han Fang Desiree Wanders Robert L. Judd The Absence of Adiponectin Alters Niacin’s Effects on Adipose Tissue Inflammation in Mice Nutrients obesity inflammation immune niacin adipokines |
| title | The Absence of Adiponectin Alters Niacin’s Effects on Adipose Tissue Inflammation in Mice |
| title_full | The Absence of Adiponectin Alters Niacin’s Effects on Adipose Tissue Inflammation in Mice |
| title_fullStr | The Absence of Adiponectin Alters Niacin’s Effects on Adipose Tissue Inflammation in Mice |
| title_full_unstemmed | The Absence of Adiponectin Alters Niacin’s Effects on Adipose Tissue Inflammation in Mice |
| title_short | The Absence of Adiponectin Alters Niacin’s Effects on Adipose Tissue Inflammation in Mice |
| title_sort | absence of adiponectin alters niacin s effects on adipose tissue inflammation in mice |
| topic | obesity inflammation immune niacin adipokines |
| url | https://www.mdpi.com/2072-6643/12/8/2427 |
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