Preparation and Complex Characterisation of Stabilised Gold Nanoparticles: Biodistribution and Application for High Resolution In Vivo Imaging
The Turkevich method was optimized to prepare gold nanoparticles (AuNP) stabilized by polyethyleneglycol (PEG) for µCT. Using various independent modalities, we thoroughly characterized the optimized PEG-AuNPs. Here, we show that PEG-AuNPs are retained in the blood and provide a high contrast in the...
| Hlavní autoři: | , , , , , , , , , |
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| Médium: | Článek |
| Jazyk: | English |
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MDPI AG
2024-11-01
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| Edice: | Pharmaceuticals |
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| On-line přístup: | https://www.mdpi.com/1424-8247/17/11/1479 |
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| author | Jaroslav Turánek Pavlína Turánek Knötigová Pavel Kulich Radim Skoupý Kamila Hrubanová Naděžda Vaškovicová Ladislav Fekete Antonín Kaňa Robert Mikulík Milan Raška |
| author_facet | Jaroslav Turánek Pavlína Turánek Knötigová Pavel Kulich Radim Skoupý Kamila Hrubanová Naděžda Vaškovicová Ladislav Fekete Antonín Kaňa Robert Mikulík Milan Raška |
| author_sort | Jaroslav Turánek |
| collection | DOAJ |
| description | The Turkevich method was optimized to prepare gold nanoparticles (AuNP) stabilized by polyethyleneglycol (PEG) for µCT. Using various independent modalities, we thoroughly characterized the optimized PEG-AuNPs. Here, we show that PEG-AuNPs are retained in the blood and provide a high contrast in the high-resolution µCT imaging of blood vessels and inner organs. The biodistribution is characterized by prolonged circulation in the blood and accumulation in the liver, spleen and skin. The accumulation of AuNP in the skin resulted in the blue discoloration of eyes and the whole skin. In vitro experiments using a leukemic monocyte THP-1 cell line model expressing high levels of NLRP3 demonstrated that the NLRP3inflammasome was not activated by PEG AuNP. Over 9 months, the mice were scanned by µCT and were in good health. Scans in mice using PEG-stabilized AuNPs in this study were sharper, with a higher contrast, when compared to a commercial contrasting agent at the same dose. The PEG-AuNPs were morphologically and chemically stable for at least two years when stored in the refrigerator. |
| first_indexed | 2025-02-18T01:00:43Z |
| format | Article |
| id | doaj.art-b3fd4f23a90e43228dc3107c07e8cb15 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2025-02-18T01:00:43Z |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-b3fd4f23a90e43228dc3107c07e8cb152024-11-26T18:17:19ZengMDPI AGPharmaceuticals1424-82472024-11-011711147910.3390/ph17111479Preparation and Complex Characterisation of Stabilised Gold Nanoparticles: Biodistribution and Application for High Resolution In Vivo ImagingJaroslav Turánek0Pavlína Turánek Knötigová1Pavel Kulich2Radim Skoupý3Kamila Hrubanová4Naděžda Vaškovicová5Ladislav Fekete6Antonín Kaňa7Robert Mikulík8Milan Raška9Neurology Department, The International Clinical Research Center ICRC of St. Anne’s University Hospital in Brno, Pekařská 53, 656 91 Brno, Czech RepublicNeurology Department, The International Clinical Research Center ICRC of St. Anne’s University Hospital in Brno, Pekařská 53, 656 91 Brno, Czech RepublicVeterinary Research Institute, v.v.i., Hudcova 296/70, 621 00 Brno, Czech RepublicInstitute of Scientific Instruments, v.v.i., AS CR, Královopolská 147, 612 00 Brno, Czech RepublicInstitute of Scientific Instruments, v.v.i., AS CR, Královopolská 147, 612 00 Brno, Czech RepublicDepartment of Histology and Embryology, Faculty of Medicine, Masaryk University, Kamenice 753/5, 62500 Brno, Czech RepublicInstitute of Physics, Czech Academy of Sciences, Na Slovance 2, 18200 Prague 8, Czech RepublicDepartment of Analytical Chemistry, University of Chemistry and Technology, Prague, Technická 5, 166 28 Praha 6, Czech RepublicNeurology Department, The International Clinical Research Center ICRC of St. Anne’s University Hospital in Brno, Pekařská 53, 656 91 Brno, Czech RepublicDepartment of Immunology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Hněvotínská 3, 775 15 Olomouc, Czech RepublicThe Turkevich method was optimized to prepare gold nanoparticles (AuNP) stabilized by polyethyleneglycol (PEG) for µCT. Using various independent modalities, we thoroughly characterized the optimized PEG-AuNPs. Here, we show that PEG-AuNPs are retained in the blood and provide a high contrast in the high-resolution µCT imaging of blood vessels and inner organs. The biodistribution is characterized by prolonged circulation in the blood and accumulation in the liver, spleen and skin. The accumulation of AuNP in the skin resulted in the blue discoloration of eyes and the whole skin. In vitro experiments using a leukemic monocyte THP-1 cell line model expressing high levels of NLRP3 demonstrated that the NLRP3inflammasome was not activated by PEG AuNP. Over 9 months, the mice were scanned by µCT and were in good health. Scans in mice using PEG-stabilized AuNPs in this study were sharper, with a higher contrast, when compared to a commercial contrasting agent at the same dose. The PEG-AuNPs were morphologically and chemically stable for at least two years when stored in the refrigerator.https://www.mdpi.com/1424-8247/17/11/1479gold nanoparticlesin vivo imagingmicrocomputer tomographybiodistributionnanotoxicology |
| spellingShingle | Jaroslav Turánek Pavlína Turánek Knötigová Pavel Kulich Radim Skoupý Kamila Hrubanová Naděžda Vaškovicová Ladislav Fekete Antonín Kaňa Robert Mikulík Milan Raška Preparation and Complex Characterisation of Stabilised Gold Nanoparticles: Biodistribution and Application for High Resolution In Vivo Imaging Pharmaceuticals gold nanoparticles in vivo imaging microcomputer tomography biodistribution nanotoxicology |
| title | Preparation and Complex Characterisation of Stabilised Gold Nanoparticles: Biodistribution and Application for High Resolution In Vivo Imaging |
| title_full | Preparation and Complex Characterisation of Stabilised Gold Nanoparticles: Biodistribution and Application for High Resolution In Vivo Imaging |
| title_fullStr | Preparation and Complex Characterisation of Stabilised Gold Nanoparticles: Biodistribution and Application for High Resolution In Vivo Imaging |
| title_full_unstemmed | Preparation and Complex Characterisation of Stabilised Gold Nanoparticles: Biodistribution and Application for High Resolution In Vivo Imaging |
| title_short | Preparation and Complex Characterisation of Stabilised Gold Nanoparticles: Biodistribution and Application for High Resolution In Vivo Imaging |
| title_sort | preparation and complex characterisation of stabilised gold nanoparticles biodistribution and application for high resolution in vivo imaging |
| topic | gold nanoparticles in vivo imaging microcomputer tomography biodistribution nanotoxicology |
| url | https://www.mdpi.com/1424-8247/17/11/1479 |
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