The effect of intrahippocampal injection of estradiol on spatial memory in male rats during multiple sclerosis disease
Background & Objective: Multiple sclerosis (MS) is a demyelinating disease of central nervous system, which is approximately two to three times more common in women than men. It was suggested that sexual hormones affect MS disease. Over half of MS patients have cognitive deficits, including lear...
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| Format: | Article |
| Language: | fas |
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Fasa University of Medical Sciences
2014-11-01
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| Series: | Journal of Fasa University of Medical Sciences |
| Subjects: | |
| Online Access: | http://journal.fums.ac.ir/browse.php?a_code=A-10-269-1&slc_lang=en&sid=1 |
| _version_ | 1811247261892476928 |
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| author | Vahideh Zarrin Homeyra Hatami Alireza Ali Hemmati |
| author_facet | Vahideh Zarrin Homeyra Hatami Alireza Ali Hemmati |
| author_sort | Vahideh Zarrin |
| collection | DOAJ |
| description | Background & Objective: Multiple sclerosis (MS) is a demyelinating disease of
central nervous system, which is approximately two to three times more common
in women than men. It was suggested that sexual hormones affect MS disease.
Over half of MS patients have cognitive deficits, including learning and memory
dysfunction. Estrogen also influences most of the cerebral function including
learning and memory. According to the involvement of estrogen on learning and
memory process, the objective of this study is to investigate the
neuroprotective effect of estrogen during MS disease. Materials & Methods: 28 male rats after cannnulating into the CA1 area of
hippocampus, randomly divided into four groups (n=7) including control group,
sham group (sesame oil, 1 μl/rat), MS group (0.01% ethidium bromide, 3 μl/rat),
MS + estradiol group (ethidium bromide + estradiol benzoate, 2 μg/1μl). MS
model was induced by intra hippocampal injection of ethidium bromide and
estradiol benzoate was injected as a pretreatment for five days. The Morris
Water Maze test was used for studying spatial memory. Data analysis was
performed using one-way ANOVA and Tukey test. Results: Ethidium bromide reduced the spatial memory (P<0.05).
Pretreatment of estradiol benzoate (2 μg/1μl) significantly improved spatial
memory during MS disease (P<0.05). Conclusion: It seems that estradiol benzoate
improves the reduced spatial memory during MS disease due to its
neuroprotective effects. |
| first_indexed | 2024-04-12T15:05:47Z |
| format | Article |
| id | doaj.art-b3feaa79d70a4675b51d6e0cb4c12d28 |
| institution | Directory Open Access Journal |
| issn | 2228-5105 2228-7329 |
| language | fas |
| last_indexed | 2024-04-12T15:05:47Z |
| publishDate | 2014-11-01 |
| publisher | Fasa University of Medical Sciences |
| record_format | Article |
| series | Journal of Fasa University of Medical Sciences |
| spelling | doaj.art-b3feaa79d70a4675b51d6e0cb4c12d282022-12-22T03:27:56ZfasFasa University of Medical SciencesJournal of Fasa University of Medical Sciences2228-51052228-73292014-11-0143353361The effect of intrahippocampal injection of estradiol on spatial memory in male rats during multiple sclerosis diseaseVahideh Zarrin0Homeyra Hatami1Alireza Ali Hemmati2 Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran Department of Anatomy and Histology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Background & Objective: Multiple sclerosis (MS) is a demyelinating disease of central nervous system, which is approximately two to three times more common in women than men. It was suggested that sexual hormones affect MS disease. Over half of MS patients have cognitive deficits, including learning and memory dysfunction. Estrogen also influences most of the cerebral function including learning and memory. According to the involvement of estrogen on learning and memory process, the objective of this study is to investigate the neuroprotective effect of estrogen during MS disease. Materials & Methods: 28 male rats after cannnulating into the CA1 area of hippocampus, randomly divided into four groups (n=7) including control group, sham group (sesame oil, 1 μl/rat), MS group (0.01% ethidium bromide, 3 μl/rat), MS + estradiol group (ethidium bromide + estradiol benzoate, 2 μg/1μl). MS model was induced by intra hippocampal injection of ethidium bromide and estradiol benzoate was injected as a pretreatment for five days. The Morris Water Maze test was used for studying spatial memory. Data analysis was performed using one-way ANOVA and Tukey test. Results: Ethidium bromide reduced the spatial memory (P<0.05). Pretreatment of estradiol benzoate (2 μg/1μl) significantly improved spatial memory during MS disease (P<0.05). Conclusion: It seems that estradiol benzoate improves the reduced spatial memory during MS disease due to its neuroprotective effects.http://journal.fums.ac.ir/browse.php?a_code=A-10-269-1&slc_lang=en&sid=1Estradiol Intra hippocampal injection Multiple sclerosis Spatial memory Rat |
| spellingShingle | Vahideh Zarrin Homeyra Hatami Alireza Ali Hemmati The effect of intrahippocampal injection of estradiol on spatial memory in male rats during multiple sclerosis disease Journal of Fasa University of Medical Sciences Estradiol Intra hippocampal injection Multiple sclerosis Spatial memory Rat |
| title | The effect of intrahippocampal injection of estradiol on spatial memory in male rats during multiple sclerosis disease |
| title_full | The effect of intrahippocampal injection of estradiol on spatial memory in male rats during multiple sclerosis disease |
| title_fullStr | The effect of intrahippocampal injection of estradiol on spatial memory in male rats during multiple sclerosis disease |
| title_full_unstemmed | The effect of intrahippocampal injection of estradiol on spatial memory in male rats during multiple sclerosis disease |
| title_short | The effect of intrahippocampal injection of estradiol on spatial memory in male rats during multiple sclerosis disease |
| title_sort | effect of intrahippocampal injection of estradiol on spatial memory in male rats during multiple sclerosis disease |
| topic | Estradiol Intra hippocampal injection Multiple sclerosis Spatial memory Rat |
| url | http://journal.fums.ac.ir/browse.php?a_code=A-10-269-1&slc_lang=en&sid=1 |
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