The phytochemical and pharmacological screening of three crude extracts of Desmodium canum (strong back)
Abstract Introduction Desmodium canum (Strong Back) is deemed a versatile traditional medicine, where it is used to treat diabetes, hypertension, asthma and erectile dysfunction. Aim To identify the various phytochemicals present within extracts of D. canum, their antioxidant capabilities and their...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-11-01
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| Series: | Clinical Phytoscience |
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| Online Access: | http://link.springer.com/article/10.1186/s40816-020-00220-w |
| _version_ | 1818187875894689792 |
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| author | Ruby Lisa Alexander-Lindo Roy Barrington Reynolds Porter Chuckwuemeka Rapheal Nwokocha Kemmoy Godfrey Lattibeaudiere |
| author_facet | Ruby Lisa Alexander-Lindo Roy Barrington Reynolds Porter Chuckwuemeka Rapheal Nwokocha Kemmoy Godfrey Lattibeaudiere |
| author_sort | Ruby Lisa Alexander-Lindo |
| collection | DOAJ |
| description | Abstract Introduction Desmodium canum (Strong Back) is deemed a versatile traditional medicine, where it is used to treat diabetes, hypertension, asthma and erectile dysfunction. Aim To identify the various phytochemicals present within extracts of D. canum, their antioxidant capabilities and their effects on blood glucose levels, haemodynamic parameters and testosterone levels in healthy Sprague-Dawley (S-D) rats. Method Crude extracts were obtained using hexane, ethyl acetate and methanol. These were analysed for various phytochemicals and their antioxidant potential assessed using the 2,2-diphenyl-1picrylhydrazyl (DPPH) method. The extracts were investigated for hypoglycaemic potential using the Oral Glucose Tolerance Test (OGTT), where extracts were administered intravenously (50 mg/kg BW) to fasted rats and their blood glucose readings monitored at 30 min intervals. The hypotensive effect of the extracts were also investigated where rats were administered intravenously at 50 mg/kg BW. These haemodynamic parameters were monitored using the CODA 6 machine at 5 min intervals for a total of 20 min. Additionally, the effect on testosterone level was investigated in male rats where extracts were administered daily by oral gavage. Serum testosterone levels were then determined using an ELISA kit. Results The different extracts showed varying phytochemical and pharmacological properties. The methanol extract showed antioxidant capabilities, while the ethyl acetate extract showed significant hypoglycaemic and hypotensive effects when compared with the control. The hexane extract showed significant activity in increasing the testosterone when compared with the control. Conclusion D. canum extracts showed significant pharmacological activities in normal Sprague- Dawley rats. |
| first_indexed | 2024-12-11T23:17:59Z |
| format | Article |
| id | doaj.art-b403107410e74eff92f12ad0a6b7fb5a |
| institution | Directory Open Access Journal |
| issn | 2199-1197 |
| language | English |
| last_indexed | 2024-12-11T23:17:59Z |
| publishDate | 2020-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Clinical Phytoscience |
| spelling | doaj.art-b403107410e74eff92f12ad0a6b7fb5a2022-12-22T00:46:27ZengSpringerOpenClinical Phytoscience2199-11972020-11-01611910.1186/s40816-020-00220-wThe phytochemical and pharmacological screening of three crude extracts of Desmodium canum (strong back)Ruby Lisa Alexander-Lindo0Roy Barrington Reynolds Porter1Chuckwuemeka Rapheal Nwokocha2Kemmoy Godfrey Lattibeaudiere3Department of Basic Medical Sciences, Biochemistry Section, Faculty of Medical Sciences, The University of the West IndiesDepartment of Chemistry, The University of the West IndiesDepartment of Basic Medical Sciences, Physiology Section, The University of the West IndiesDepartment of Basic Medical Sciences, Biochemistry Section, Faculty of Medical Sciences, The University of the West IndiesAbstract Introduction Desmodium canum (Strong Back) is deemed a versatile traditional medicine, where it is used to treat diabetes, hypertension, asthma and erectile dysfunction. Aim To identify the various phytochemicals present within extracts of D. canum, their antioxidant capabilities and their effects on blood glucose levels, haemodynamic parameters and testosterone levels in healthy Sprague-Dawley (S-D) rats. Method Crude extracts were obtained using hexane, ethyl acetate and methanol. These were analysed for various phytochemicals and their antioxidant potential assessed using the 2,2-diphenyl-1picrylhydrazyl (DPPH) method. The extracts were investigated for hypoglycaemic potential using the Oral Glucose Tolerance Test (OGTT), where extracts were administered intravenously (50 mg/kg BW) to fasted rats and their blood glucose readings monitored at 30 min intervals. The hypotensive effect of the extracts were also investigated where rats were administered intravenously at 50 mg/kg BW. These haemodynamic parameters were monitored using the CODA 6 machine at 5 min intervals for a total of 20 min. Additionally, the effect on testosterone level was investigated in male rats where extracts were administered daily by oral gavage. Serum testosterone levels were then determined using an ELISA kit. Results The different extracts showed varying phytochemical and pharmacological properties. The methanol extract showed antioxidant capabilities, while the ethyl acetate extract showed significant hypoglycaemic and hypotensive effects when compared with the control. The hexane extract showed significant activity in increasing the testosterone when compared with the control. Conclusion D. canum extracts showed significant pharmacological activities in normal Sprague- Dawley rats.http://link.springer.com/article/10.1186/s40816-020-00220-wDesmonium canumStrong backHypoglycaemicHypotensiveOral glucose tolerance test (OGTT)CODA 6 |
| spellingShingle | Ruby Lisa Alexander-Lindo Roy Barrington Reynolds Porter Chuckwuemeka Rapheal Nwokocha Kemmoy Godfrey Lattibeaudiere The phytochemical and pharmacological screening of three crude extracts of Desmodium canum (strong back) Clinical Phytoscience Desmonium canum Strong back Hypoglycaemic Hypotensive Oral glucose tolerance test (OGTT) CODA 6 |
| title | The phytochemical and pharmacological screening of three crude extracts of Desmodium canum (strong back) |
| title_full | The phytochemical and pharmacological screening of three crude extracts of Desmodium canum (strong back) |
| title_fullStr | The phytochemical and pharmacological screening of three crude extracts of Desmodium canum (strong back) |
| title_full_unstemmed | The phytochemical and pharmacological screening of three crude extracts of Desmodium canum (strong back) |
| title_short | The phytochemical and pharmacological screening of three crude extracts of Desmodium canum (strong back) |
| title_sort | phytochemical and pharmacological screening of three crude extracts of desmodium canum strong back |
| topic | Desmonium canum Strong back Hypoglycaemic Hypotensive Oral glucose tolerance test (OGTT) CODA 6 |
| url | http://link.springer.com/article/10.1186/s40816-020-00220-w |
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