Leukocyte CH25H is a potential diagnostic and prognostic marker for lung adenocarcinoma
Abstract Metastasis, a major challenge during the treatment of lung cancer, causes deterioration in patient health outcomes. Thus, to address this problem, this study aimed to explore the role and contribution of Cholesterol 25-Hydroxylase (CH25H) as a potential diagnostic and prognostic marker in l...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-12-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-24183-9 |
| _version_ | 1797977494958112768 |
|---|---|
| author | Jun Zhang Lidong Xu Jun Gao Jieyi Li Xiaokai Zhao Pengmin Yang Yujun Ge Dawei Guo Zhonghua Liu Xiyong Wang Wenchao Gu Pengli Wang Xun Hu Ziying Gong Daoyun Zhang |
| author_facet | Jun Zhang Lidong Xu Jun Gao Jieyi Li Xiaokai Zhao Pengmin Yang Yujun Ge Dawei Guo Zhonghua Liu Xiyong Wang Wenchao Gu Pengli Wang Xun Hu Ziying Gong Daoyun Zhang |
| author_sort | Jun Zhang |
| collection | DOAJ |
| description | Abstract Metastasis, a major challenge during the treatment of lung cancer, causes deterioration in patient health outcomes. Thus, to address this problem, this study aimed to explore the role and contribution of Cholesterol 25-Hydroxylase (CH25H) as a potential diagnostic and prognostic marker in lung cancer. Online public databases were used to analyze the expression level, prognostic value, gene-pathway enrichment, and immune infiltration of CH25H in lung cancer patients. The Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) was used to analyze and detect the CH25H expression levels in leukocytes from lung cancer patients. The expression level of CH25H was significantly reduced in lung adenocarcinoma (LUAD), which is associated with a higher disease stage, but not in lung squamous cell carcinoma (LUSC). Kaplan–Meier survival analysis indicated that LUAD patients with low CH25H expression had a worse prognosis. Mechanistically, our results showed that in LUAD, CH25H may be a regulatory factor affecting the immune cell infiltration level, and the resultant tumor development. Experimental data showed that low expression of CH25H in leukocytes was significantly associated with LUAD metastasis (P < 0.01). Our study suggests that CH25H may function as a prognostic and risk stratification biomarker for LUAD. |
| first_indexed | 2024-04-11T05:08:52Z |
| format | Article |
| id | doaj.art-b4066044bc3b4d8eb6293e4c99c45e74 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T05:08:52Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b4066044bc3b4d8eb6293e4c99c45e742022-12-25T12:11:42ZengNature PortfolioScientific Reports2045-23222022-12-011211910.1038/s41598-022-24183-9Leukocyte CH25H is a potential diagnostic and prognostic marker for lung adenocarcinomaJun Zhang0Lidong Xu1Jun Gao2Jieyi Li3Xiaokai Zhao4Pengmin Yang5Yujun Ge6Dawei Guo7Zhonghua Liu8Xiyong Wang9Wenchao Gu10Pengli Wang11Xun Hu12Ziying Gong13Daoyun Zhang14Department of Cardiothoracic Surgery, The Second Affiliated Hospital of Jiaxing UniversityDepartment of Cardiothoracic Surgery, The Second Affiliated Hospital of Jiaxing UniversityDepartment of Cardiothoracic Surgery, The Second Affiliated Hospital of Jiaxing UniversityJiaxing Key Laboratory of Precision Medicine and Companion Diagnostics, Jiaxing Yunying Medical Inspection Co., Ltd.Jiaxing Key Laboratory of Precision Medicine and Companion Diagnostics, Jiaxing Yunying Medical Inspection Co., Ltd.Jiaxing Key Laboratory of Precision Medicine and Companion Diagnostics, Jiaxing Yunying Medical Inspection Co., Ltd.Department of Cardiothoracic Surgery, The Second Affiliated Hospital of Jiaxing UniversityDepartment of Cardiothoracic Surgery, The Second Affiliated Hospital of Jiaxing UniversityDepartment of Cardiothoracic Surgery, The Second Affiliated Hospital of Jiaxing UniversityDepartment of Cardiothoracic Surgery, The Second Affiliated Hospital of Jiaxing UniversityDepartment of Cardiothoracic Surgery, The Second Affiliated Hospital of Jiaxing UniversityDepartment of Cardiothoracic Surgery, The Second Affiliated Hospital of Jiaxing UniversityDepartment of Cardiothoracic Surgery, The Second Affiliated Hospital of Jiaxing UniversityJiaxing Key Laboratory of Precision Medicine and Companion Diagnostics, Jiaxing Yunying Medical Inspection Co., Ltd.Jiaxing Key Laboratory of Precision Medicine and Companion Diagnostics, Jiaxing Yunying Medical Inspection Co., Ltd.Abstract Metastasis, a major challenge during the treatment of lung cancer, causes deterioration in patient health outcomes. Thus, to address this problem, this study aimed to explore the role and contribution of Cholesterol 25-Hydroxylase (CH25H) as a potential diagnostic and prognostic marker in lung cancer. Online public databases were used to analyze the expression level, prognostic value, gene-pathway enrichment, and immune infiltration of CH25H in lung cancer patients. The Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) was used to analyze and detect the CH25H expression levels in leukocytes from lung cancer patients. The expression level of CH25H was significantly reduced in lung adenocarcinoma (LUAD), which is associated with a higher disease stage, but not in lung squamous cell carcinoma (LUSC). Kaplan–Meier survival analysis indicated that LUAD patients with low CH25H expression had a worse prognosis. Mechanistically, our results showed that in LUAD, CH25H may be a regulatory factor affecting the immune cell infiltration level, and the resultant tumor development. Experimental data showed that low expression of CH25H in leukocytes was significantly associated with LUAD metastasis (P < 0.01). Our study suggests that CH25H may function as a prognostic and risk stratification biomarker for LUAD.https://doi.org/10.1038/s41598-022-24183-9 |
| spellingShingle | Jun Zhang Lidong Xu Jun Gao Jieyi Li Xiaokai Zhao Pengmin Yang Yujun Ge Dawei Guo Zhonghua Liu Xiyong Wang Wenchao Gu Pengli Wang Xun Hu Ziying Gong Daoyun Zhang Leukocyte CH25H is a potential diagnostic and prognostic marker for lung adenocarcinoma Scientific Reports |
| title | Leukocyte CH25H is a potential diagnostic and prognostic marker for lung adenocarcinoma |
| title_full | Leukocyte CH25H is a potential diagnostic and prognostic marker for lung adenocarcinoma |
| title_fullStr | Leukocyte CH25H is a potential diagnostic and prognostic marker for lung adenocarcinoma |
| title_full_unstemmed | Leukocyte CH25H is a potential diagnostic and prognostic marker for lung adenocarcinoma |
| title_short | Leukocyte CH25H is a potential diagnostic and prognostic marker for lung adenocarcinoma |
| title_sort | leukocyte ch25h is a potential diagnostic and prognostic marker for lung adenocarcinoma |
| url | https://doi.org/10.1038/s41598-022-24183-9 |
| work_keys_str_mv | AT junzhang leukocytech25hisapotentialdiagnosticandprognosticmarkerforlungadenocarcinoma AT lidongxu leukocytech25hisapotentialdiagnosticandprognosticmarkerforlungadenocarcinoma AT jungao leukocytech25hisapotentialdiagnosticandprognosticmarkerforlungadenocarcinoma AT jieyili leukocytech25hisapotentialdiagnosticandprognosticmarkerforlungadenocarcinoma AT xiaokaizhao leukocytech25hisapotentialdiagnosticandprognosticmarkerforlungadenocarcinoma AT pengminyang leukocytech25hisapotentialdiagnosticandprognosticmarkerforlungadenocarcinoma AT yujunge leukocytech25hisapotentialdiagnosticandprognosticmarkerforlungadenocarcinoma AT daweiguo leukocytech25hisapotentialdiagnosticandprognosticmarkerforlungadenocarcinoma AT zhonghualiu leukocytech25hisapotentialdiagnosticandprognosticmarkerforlungadenocarcinoma AT xiyongwang leukocytech25hisapotentialdiagnosticandprognosticmarkerforlungadenocarcinoma AT wenchaogu leukocytech25hisapotentialdiagnosticandprognosticmarkerforlungadenocarcinoma AT pengliwang leukocytech25hisapotentialdiagnosticandprognosticmarkerforlungadenocarcinoma AT xunhu leukocytech25hisapotentialdiagnosticandprognosticmarkerforlungadenocarcinoma AT ziyinggong leukocytech25hisapotentialdiagnosticandprognosticmarkerforlungadenocarcinoma AT daoyunzhang leukocytech25hisapotentialdiagnosticandprognosticmarkerforlungadenocarcinoma |