Maternal sleep deprivation inhibits hippocampal neurogenesis associated with inflammatory response in young offspring rats
Although sleep complaints are very common among pregnant women, the potential adverse effects of sleep disturbance on the offspring are not well studied. Growing evidence suggests that maternal stress can induce an inflammatory environment on the fetal development. But people are not sure about the...
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| Format: | Article |
| Language: | English |
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Elsevier
2014-08-01
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| Series: | Neurobiology of Disease |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0969996114000977 |
| _version_ | 1818742097414455296 |
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| author | Qiuying Zhao Cheng Peng Xiaohui Wu Yubo Chen Cheng Wang Zili You |
| author_facet | Qiuying Zhao Cheng Peng Xiaohui Wu Yubo Chen Cheng Wang Zili You |
| author_sort | Qiuying Zhao |
| collection | DOAJ |
| description | Although sleep complaints are very common among pregnant women, the potential adverse effects of sleep disturbance on the offspring are not well studied. Growing evidence suggests that maternal stress can induce an inflammatory environment on the fetal development. But people are not sure about the consequences of prenatal stress such as the inflammatory responses induced by maternal sleep deprivation (MSD). In the present study, we investigated the effects of MSD on long-term behavioral and cognitive consequences in offspring and its underlying inflammatory response pathway. The pregnant Wistar rats received prolonged sleep deprivation (72 h) on gestational day (GD) 4, 9, and 18, respectively. The post-natal day (PND) 21 offspring showed impaired hippocampus-dependent spatial learning and memory in the Morris Water Maze task and anhedonia in sucrose preference experiment. Quantification of BrdU+ and DCX+ cells revealed a significant decrease in hippocampus neurogenesis in prepuberty offspring, especially for the late MSD (GD 18) group. Real-time RT-PCR showed that after MSD, the expression of pro-inflammatory cytokines (IL-1β, IL-6 and TNFα) increased in the hippocampus of offspring on PND 1, 7, 14 and 21, whereas anti-inflammatory cytokine IL-10 reduced at the same time. Immunofluorescence found that the cells of activated microglia were higher in the brains of MSD offspring. Taken together, these results suggested that the MSD-induced inflammatory response is an important factor for neurogenesis impairment and neurobehavioral outcomes in prepuberty offspring. |
| first_indexed | 2024-12-18T02:07:06Z |
| format | Article |
| id | doaj.art-b40cfdfa84de4728b1e92f0298ec9407 |
| institution | Directory Open Access Journal |
| issn | 1095-953X |
| language | English |
| last_indexed | 2024-12-18T02:07:06Z |
| publishDate | 2014-08-01 |
| publisher | Elsevier |
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| series | Neurobiology of Disease |
| spelling | doaj.art-b40cfdfa84de4728b1e92f0298ec94072022-12-21T21:24:34ZengElsevierNeurobiology of Disease1095-953X2014-08-01685765Maternal sleep deprivation inhibits hippocampal neurogenesis associated with inflammatory response in young offspring ratsQiuying Zhao0Cheng Peng1Xiaohui Wu2Yubo Chen3Cheng Wang4Zili You5School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, ChinaState Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, ChinaSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, ChinaSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, ChinaSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, ChinaSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China; Corresponding author. Fax: +86 28 83208838.Although sleep complaints are very common among pregnant women, the potential adverse effects of sleep disturbance on the offspring are not well studied. Growing evidence suggests that maternal stress can induce an inflammatory environment on the fetal development. But people are not sure about the consequences of prenatal stress such as the inflammatory responses induced by maternal sleep deprivation (MSD). In the present study, we investigated the effects of MSD on long-term behavioral and cognitive consequences in offspring and its underlying inflammatory response pathway. The pregnant Wistar rats received prolonged sleep deprivation (72 h) on gestational day (GD) 4, 9, and 18, respectively. The post-natal day (PND) 21 offspring showed impaired hippocampus-dependent spatial learning and memory in the Morris Water Maze task and anhedonia in sucrose preference experiment. Quantification of BrdU+ and DCX+ cells revealed a significant decrease in hippocampus neurogenesis in prepuberty offspring, especially for the late MSD (GD 18) group. Real-time RT-PCR showed that after MSD, the expression of pro-inflammatory cytokines (IL-1β, IL-6 and TNFα) increased in the hippocampus of offspring on PND 1, 7, 14 and 21, whereas anti-inflammatory cytokine IL-10 reduced at the same time. Immunofluorescence found that the cells of activated microglia were higher in the brains of MSD offspring. Taken together, these results suggested that the MSD-induced inflammatory response is an important factor for neurogenesis impairment and neurobehavioral outcomes in prepuberty offspring.http://www.sciencedirect.com/science/article/pii/S0969996114000977Sleep deprivationPregnancyOffspringNeurogenesisMicroglial activationInflammatory response |
| spellingShingle | Qiuying Zhao Cheng Peng Xiaohui Wu Yubo Chen Cheng Wang Zili You Maternal sleep deprivation inhibits hippocampal neurogenesis associated with inflammatory response in young offspring rats Neurobiology of Disease Sleep deprivation Pregnancy Offspring Neurogenesis Microglial activation Inflammatory response |
| title | Maternal sleep deprivation inhibits hippocampal neurogenesis associated with inflammatory response in young offspring rats |
| title_full | Maternal sleep deprivation inhibits hippocampal neurogenesis associated with inflammatory response in young offspring rats |
| title_fullStr | Maternal sleep deprivation inhibits hippocampal neurogenesis associated with inflammatory response in young offspring rats |
| title_full_unstemmed | Maternal sleep deprivation inhibits hippocampal neurogenesis associated with inflammatory response in young offspring rats |
| title_short | Maternal sleep deprivation inhibits hippocampal neurogenesis associated with inflammatory response in young offspring rats |
| title_sort | maternal sleep deprivation inhibits hippocampal neurogenesis associated with inflammatory response in young offspring rats |
| topic | Sleep deprivation Pregnancy Offspring Neurogenesis Microglial activation Inflammatory response |
| url | http://www.sciencedirect.com/science/article/pii/S0969996114000977 |
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