Antimicrobial activity of the Lacticaseibacillus rhamnosus CRL 2244 and its impact on the phenotypic and transcriptional responses in carbapenem resistant Acinetobacter baumannii
Abstract Carbapenem-resistant Acinetobacter baumannii (CRAB) is a recognized nosocomial pathogen with limited antibiotic treatment options. Lactic acid bacteria (LAB) constitute a promising therapeutic alternative. Here we studied the antibacterial properties of a collection of LAB strains using phe...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-41334-8 |
| _version_ | 1797453360799940608 |
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| author | Cecilia Rodriguez Dema Ramlaoui Nardin Georgeos Briea Gasca Camila Leal Tomás Subils Marisel R. Tuttobene Rodrigo Sieira Nicholas T. Salzameda Robert A. Bonomo Raúl Raya María Soledad Ramirez |
| author_facet | Cecilia Rodriguez Dema Ramlaoui Nardin Georgeos Briea Gasca Camila Leal Tomás Subils Marisel R. Tuttobene Rodrigo Sieira Nicholas T. Salzameda Robert A. Bonomo Raúl Raya María Soledad Ramirez |
| author_sort | Cecilia Rodriguez |
| collection | DOAJ |
| description | Abstract Carbapenem-resistant Acinetobacter baumannii (CRAB) is a recognized nosocomial pathogen with limited antibiotic treatment options. Lactic acid bacteria (LAB) constitute a promising therapeutic alternative. Here we studied the antibacterial properties of a collection of LAB strains using phenotypic and transcriptomic analysis against A. baumannii clinical strains. One strain, Lacticaseibacillus rhamnosus CRL 2244, demonstrated a potent inhibitory capacity on A. baumannii with a significant killing activity. Scanning electron microscopy images showed changes in the morphology of A. baumannii with an increased formation of outer membrane vesicles. Significant changes in the expression levels of a wide variety of genes were also observed. Interestingly, most of the modified genes were involved in a metabolic pathway known to be associated with the survival of A. baumannii. The paa operon, Hut system, and fatty acid degradation were some of the pathways that were induced. The analysis reveals the impact of Lcb. rhamnosus CRL 2244 on A. baumannii response, resulting in bacterial stress and subsequent cell death. These findings highlight the antibacterial properties of Lcb. rhamnosus CRL 2244 and its potential as an alternative or complementary strategy for treating infections. Further exploration and development of LAB as a treatment option could provide valuable alternatives for combating CRAB infections. |
| first_indexed | 2024-03-09T15:21:41Z |
| format | Article |
| id | doaj.art-b40dd5a3884e4382b2014e363efb0f62 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:21:41Z |
| publishDate | 2023-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b40dd5a3884e4382b2014e363efb0f622023-11-26T12:48:03ZengNature PortfolioScientific Reports2045-23222023-08-0113111310.1038/s41598-023-41334-8Antimicrobial activity of the Lacticaseibacillus rhamnosus CRL 2244 and its impact on the phenotypic and transcriptional responses in carbapenem resistant Acinetobacter baumanniiCecilia Rodriguez0Dema Ramlaoui1Nardin Georgeos2Briea Gasca3Camila Leal4Tomás Subils5Marisel R. Tuttobene6Rodrigo Sieira7Nicholas T. Salzameda8Robert A. Bonomo9Raúl Raya10María Soledad Ramirez11Centro de Referencia Para Lactobacilos (CERELA), CONICETCenter for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University Fullerton (CSUF)Center for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University Fullerton (CSUF)Center for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University Fullerton (CSUF)Centro de Referencia Para Lactobacilos (CERELA), CONICETInstituto de Procesos Biotecnológicos y Químicos de Rosario (IPROBYQ, CONICET-UNR)Instituto de Biología Molecular y Celular de Rosario (IBR, CONICET-UNR)Fundación Instituto Leloir-IIBBA CONICETDepartment of Chemistry and Biochemistry, College of Natural Science and Mathematics, CSUFResearch Service and GRECC, Department of Veterans Affairs Medical Center, Louis Stokes ClevelandCentro de Referencia Para Lactobacilos (CERELA), CONICETCenter for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University Fullerton (CSUF)Abstract Carbapenem-resistant Acinetobacter baumannii (CRAB) is a recognized nosocomial pathogen with limited antibiotic treatment options. Lactic acid bacteria (LAB) constitute a promising therapeutic alternative. Here we studied the antibacterial properties of a collection of LAB strains using phenotypic and transcriptomic analysis against A. baumannii clinical strains. One strain, Lacticaseibacillus rhamnosus CRL 2244, demonstrated a potent inhibitory capacity on A. baumannii with a significant killing activity. Scanning electron microscopy images showed changes in the morphology of A. baumannii with an increased formation of outer membrane vesicles. Significant changes in the expression levels of a wide variety of genes were also observed. Interestingly, most of the modified genes were involved in a metabolic pathway known to be associated with the survival of A. baumannii. The paa operon, Hut system, and fatty acid degradation were some of the pathways that were induced. The analysis reveals the impact of Lcb. rhamnosus CRL 2244 on A. baumannii response, resulting in bacterial stress and subsequent cell death. These findings highlight the antibacterial properties of Lcb. rhamnosus CRL 2244 and its potential as an alternative or complementary strategy for treating infections. Further exploration and development of LAB as a treatment option could provide valuable alternatives for combating CRAB infections.https://doi.org/10.1038/s41598-023-41334-8 |
| spellingShingle | Cecilia Rodriguez Dema Ramlaoui Nardin Georgeos Briea Gasca Camila Leal Tomás Subils Marisel R. Tuttobene Rodrigo Sieira Nicholas T. Salzameda Robert A. Bonomo Raúl Raya María Soledad Ramirez Antimicrobial activity of the Lacticaseibacillus rhamnosus CRL 2244 and its impact on the phenotypic and transcriptional responses in carbapenem resistant Acinetobacter baumannii Scientific Reports |
| title | Antimicrobial activity of the Lacticaseibacillus rhamnosus CRL 2244 and its impact on the phenotypic and transcriptional responses in carbapenem resistant Acinetobacter baumannii |
| title_full | Antimicrobial activity of the Lacticaseibacillus rhamnosus CRL 2244 and its impact on the phenotypic and transcriptional responses in carbapenem resistant Acinetobacter baumannii |
| title_fullStr | Antimicrobial activity of the Lacticaseibacillus rhamnosus CRL 2244 and its impact on the phenotypic and transcriptional responses in carbapenem resistant Acinetobacter baumannii |
| title_full_unstemmed | Antimicrobial activity of the Lacticaseibacillus rhamnosus CRL 2244 and its impact on the phenotypic and transcriptional responses in carbapenem resistant Acinetobacter baumannii |
| title_short | Antimicrobial activity of the Lacticaseibacillus rhamnosus CRL 2244 and its impact on the phenotypic and transcriptional responses in carbapenem resistant Acinetobacter baumannii |
| title_sort | antimicrobial activity of the lacticaseibacillus rhamnosus crl 2244 and its impact on the phenotypic and transcriptional responses in carbapenem resistant acinetobacter baumannii |
| url | https://doi.org/10.1038/s41598-023-41334-8 |
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