Isolation, identification, molecular typing, and drug resistance of Escherichia coli from infected cattle and sheep in Xinjiang, China
Abstract Background Escherichia coli infections are common in Xinjiang, a major region of cattle and sheep breeding in China. Therefore, strategies are required to control E. coli. The aim of this study was to investigate the phylogenetic groups, virulence genes, and antibiotic resistance characteri...
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| Format: | Article |
| Language: | English |
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Wiley
2023-05-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.1101 |
| _version_ | 1797825833105096704 |
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| author | Xiaoxiao Gu Xue Ma Qin Wu Qiaoxiaoci Tao Yingjin Chai Xia Zhou Mengli Han Jie Li Xin Huang Tongzhong Wu Xingxing Zhang Fagang Zhong Yiheng Cao Liyuan Zhang |
| author_facet | Xiaoxiao Gu Xue Ma Qin Wu Qiaoxiaoci Tao Yingjin Chai Xia Zhou Mengli Han Jie Li Xin Huang Tongzhong Wu Xingxing Zhang Fagang Zhong Yiheng Cao Liyuan Zhang |
| author_sort | Xiaoxiao Gu |
| collection | DOAJ |
| description | Abstract Background Escherichia coli infections are common in Xinjiang, a major region of cattle and sheep breeding in China. Therefore, strategies are required to control E. coli. The aim of this study was to investigate the phylogenetic groups, virulence genes, and antibiotic resistance characteristics of E. coli isolates. Methods In this study, 116 tissue samples were collected from the organs of cattle and sheep that were suspected of having E. coli infections between 2015 and 2019. Bacteria in the samples were identified using a biochemical identification system and amplification of 16S rRNA, and the phylogenetic groupings of E. coli isolates were determined by multiplex polymerase chain reactions. In addition, PCR detection and analysis of virulence factors, antibiotic resistance genes, and drug‐resistant phenotypes of E. coli isolates were performed. Results A total of 116 pathogenic E. coli strains belonging to seven phylogenetic groups were isolated, with the majority of isolates in groups A and B1. Among the virulence genes, curli‐encoding crl had the highest detection rate of 97.4%, followed by hemolysin‐encoding hlyE with the detection rate of 94.82%. Antimicrobial susceptibility test results indicated that the isolates had the highest rates of resistance against streptomycin (81.9%). Conclusion These characteristics complicate the prevention and treatment of E. coli‐related diseases in Xinjiang. |
| first_indexed | 2024-03-13T10:59:43Z |
| format | Article |
| id | doaj.art-b410e9603a41419b827401662214055e |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-03-13T10:59:43Z |
| publishDate | 2023-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-b410e9603a41419b827401662214055e2023-05-16T19:51:19ZengWileyVeterinary Medicine and Science2053-10952023-05-01931359136810.1002/vms3.1101Isolation, identification, molecular typing, and drug resistance of Escherichia coli from infected cattle and sheep in Xinjiang, ChinaXiaoxiao Gu0Xue Ma1Qin Wu2Qiaoxiaoci Tao3Yingjin Chai4Xia Zhou5Mengli Han6Jie Li7Xin Huang8Tongzhong Wu9Xingxing Zhang10Fagang Zhong11Yiheng Cao12Liyuan Zhang13College of Animal Science and Technology Shihezi University Shihezi ChinaCollege of Animal Science and Technology Shihezi University Shihezi ChinaCollege of Animal Science and Technology Shihezi University Shihezi ChinaCollege of Animal Science and Technology Shihezi University Shihezi ChinaCollege of Animal Science and Technology Shihezi University Shihezi ChinaCollege of Animal Science and Technology Shihezi University Shihezi ChinaState Key Laboratory for Sheep Genetic Improvement and Healthy Production Xinjiang Academy of Agricultural and Reclamation Science Shihezi ChinaCollege of Animal Science and Technology Shihezi University Shihezi ChinaState Key Laboratory for Sheep Genetic Improvement and Healthy Production Xinjiang Academy of Agricultural and Reclamation Science Shihezi ChinaState Key Laboratory for Sheep Genetic Improvement and Healthy Production Xinjiang Academy of Agricultural and Reclamation Science Shihezi ChinaState Key Laboratory for Sheep Genetic Improvement and Healthy Production Xinjiang Academy of Agricultural and Reclamation Science Shihezi ChinaState Key Laboratory for Sheep Genetic Improvement and Healthy Production Xinjiang Academy of Agricultural and Reclamation Science Shihezi ChinaCollege of Animal Science and Technology Shihezi University Shihezi ChinaCollege of Animal Science and Technology Shihezi University Shihezi ChinaAbstract Background Escherichia coli infections are common in Xinjiang, a major region of cattle and sheep breeding in China. Therefore, strategies are required to control E. coli. The aim of this study was to investigate the phylogenetic groups, virulence genes, and antibiotic resistance characteristics of E. coli isolates. Methods In this study, 116 tissue samples were collected from the organs of cattle and sheep that were suspected of having E. coli infections between 2015 and 2019. Bacteria in the samples were identified using a biochemical identification system and amplification of 16S rRNA, and the phylogenetic groupings of E. coli isolates were determined by multiplex polymerase chain reactions. In addition, PCR detection and analysis of virulence factors, antibiotic resistance genes, and drug‐resistant phenotypes of E. coli isolates were performed. Results A total of 116 pathogenic E. coli strains belonging to seven phylogenetic groups were isolated, with the majority of isolates in groups A and B1. Among the virulence genes, curli‐encoding crl had the highest detection rate of 97.4%, followed by hemolysin‐encoding hlyE with the detection rate of 94.82%. Antimicrobial susceptibility test results indicated that the isolates had the highest rates of resistance against streptomycin (81.9%). Conclusion These characteristics complicate the prevention and treatment of E. coli‐related diseases in Xinjiang.https://doi.org/10.1002/vms3.1101antibiotic resistancecattleEscherichia coli infectionsmultiplex polymerase chain reactionphylogenyvirulence factors |
| spellingShingle | Xiaoxiao Gu Xue Ma Qin Wu Qiaoxiaoci Tao Yingjin Chai Xia Zhou Mengli Han Jie Li Xin Huang Tongzhong Wu Xingxing Zhang Fagang Zhong Yiheng Cao Liyuan Zhang Isolation, identification, molecular typing, and drug resistance of Escherichia coli from infected cattle and sheep in Xinjiang, China Veterinary Medicine and Science antibiotic resistance cattle Escherichia coli infections multiplex polymerase chain reaction phylogeny virulence factors |
| title | Isolation, identification, molecular typing, and drug resistance of Escherichia coli from infected cattle and sheep in Xinjiang, China |
| title_full | Isolation, identification, molecular typing, and drug resistance of Escherichia coli from infected cattle and sheep in Xinjiang, China |
| title_fullStr | Isolation, identification, molecular typing, and drug resistance of Escherichia coli from infected cattle and sheep in Xinjiang, China |
| title_full_unstemmed | Isolation, identification, molecular typing, and drug resistance of Escherichia coli from infected cattle and sheep in Xinjiang, China |
| title_short | Isolation, identification, molecular typing, and drug resistance of Escherichia coli from infected cattle and sheep in Xinjiang, China |
| title_sort | isolation identification molecular typing and drug resistance of escherichia coli from infected cattle and sheep in xinjiang china |
| topic | antibiotic resistance cattle Escherichia coli infections multiplex polymerase chain reaction phylogeny virulence factors |
| url | https://doi.org/10.1002/vms3.1101 |
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