Serotonin is required for pharyngeal arch morphogenesis in zebrafish
Serotonin (5-HT) is not only a neurotransmitter but also a mediator of developmental processes in vertebrates. In this study, we analyzed the importance of 5-HT during zebrafish development. The expression patterns of three zebrafish tryptophan hydroxylase isoforms (Tph1A, Tph1B, Tph2), the rate-lim...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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ScienceOpen
2014-11-01
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| Series: | ScienceOpen Research |
| Online Access: | https://www.scienceopen.com/document?vid=501d70cc-221a-444f-a69e-b74f39809599 |
| _version_ | 1797896903974715392 |
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| author | Katarina Kotnik Halavaty martin.wurtele@unifesp.br Michael Bader Salim Seyfried |
| author_facet | Katarina Kotnik Halavaty martin.wurtele@unifesp.br Michael Bader Salim Seyfried |
| author_sort | Katarina Kotnik Halavaty |
| collection | DOAJ |
| description | Serotonin (5-HT) is not only a neurotransmitter but also a mediator of developmental processes in vertebrates. In this study, we analyzed the importance of 5-HT during zebrafish development. The expression patterns of three zebrafish tryptophan hydroxylase isoforms (Tph1A, Tph1B, Tph2), the rate-limiting enzymes in 5-HT synthesis, were analyzed and compared to the appearance and distribution of 5-HT. 5-HT was found in the raphe nuclei correlating with tph2 expression and in the pineal gland correlating with tph1a and tph2 expression. tph2 deficient fish generated with antisense morpholino oligonucleotides exhibited morphogenesis defects during pharyngeal arch development. The correct specification of neural crest cells was not affected in tph2 morphants as shown by the expression of early markers, but the survival and differentiation of pharyngeal arch progenitor cells were impaired. An organizing role of 5-HT in pharyngeal arch morphogenesis was suggested by a highly regular pattern of 5-HT positive cells in this tissue. Moreover, the 5-HT2B receptor was expressed in the pharyngeal arches and its pharmacological inhibition also induced defects in pharyngeal arch morphogenesis. These results support an important role of Tph2-derived serotonin as a morphogenetic factor in the development of neural crest derived tissues. |
| first_indexed | 2024-04-10T07:50:11Z |
| format | Article |
| id | doaj.art-b41199f6a1f5433da1e64f986546b2ab |
| institution | Directory Open Access Journal |
| issn | 2199-1006 |
| language | English |
| last_indexed | 2024-04-10T07:50:11Z |
| publishDate | 2014-11-01 |
| publisher | ScienceOpen |
| record_format | Article |
| series | ScienceOpen Research |
| spelling | doaj.art-b41199f6a1f5433da1e64f986546b2ab2023-02-23T10:21:05ZengScienceOpenScienceOpen Research2199-10062014-11-0110.14293/S2199-1006.1.SOR-LIFE.AWPDLZ.v1Serotonin is required for pharyngeal arch morphogenesis in zebrafishKatarina Kotnik Halavaty0martin.wurtele@unifesp.br1Michael BaderSalim Seyfried2Max Delbrück CenterMax Delbrück CenterMax Delbrück CenterSerotonin (5-HT) is not only a neurotransmitter but also a mediator of developmental processes in vertebrates. In this study, we analyzed the importance of 5-HT during zebrafish development. The expression patterns of three zebrafish tryptophan hydroxylase isoforms (Tph1A, Tph1B, Tph2), the rate-limiting enzymes in 5-HT synthesis, were analyzed and compared to the appearance and distribution of 5-HT. 5-HT was found in the raphe nuclei correlating with tph2 expression and in the pineal gland correlating with tph1a and tph2 expression. tph2 deficient fish generated with antisense morpholino oligonucleotides exhibited morphogenesis defects during pharyngeal arch development. The correct specification of neural crest cells was not affected in tph2 morphants as shown by the expression of early markers, but the survival and differentiation of pharyngeal arch progenitor cells were impaired. An organizing role of 5-HT in pharyngeal arch morphogenesis was suggested by a highly regular pattern of 5-HT positive cells in this tissue. Moreover, the 5-HT2B receptor was expressed in the pharyngeal arches and its pharmacological inhibition also induced defects in pharyngeal arch morphogenesis. These results support an important role of Tph2-derived serotonin as a morphogenetic factor in the development of neural crest derived tissues.https://www.scienceopen.com/document?vid=501d70cc-221a-444f-a69e-b74f39809599 |
| spellingShingle | Katarina Kotnik Halavaty martin.wurtele@unifesp.br Michael Bader Salim Seyfried Serotonin is required for pharyngeal arch morphogenesis in zebrafish ScienceOpen Research |
| title | Serotonin is required for pharyngeal arch morphogenesis in zebrafish |
| title_full | Serotonin is required for pharyngeal arch morphogenesis in zebrafish |
| title_fullStr | Serotonin is required for pharyngeal arch morphogenesis in zebrafish |
| title_full_unstemmed | Serotonin is required for pharyngeal arch morphogenesis in zebrafish |
| title_short | Serotonin is required for pharyngeal arch morphogenesis in zebrafish |
| title_sort | serotonin is required for pharyngeal arch morphogenesis in zebrafish |
| url | https://www.scienceopen.com/document?vid=501d70cc-221a-444f-a69e-b74f39809599 |
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